Melike Bor

The effect of salt stress on lipid peroxidation and antioxidants in leaves of sugar beet Beta vulgaris L. and wild beet Beta maritima L.

Abstract The changes in lipid peroxidation and the possible involvement of the antioxidant system in relation to the tolerance to salt stress was investigated in the cultivated beet Beta vulgaris L. cv. ansa and its wild salt-tolerant relative Beta maritima TR 51196 . The 40 days old beet seedlings were subjected to 0, 150 and 500 mM NaCl for 12 days. In B. maritima constitutive level of lipid peroxidation was lower, but activities of superoxide dismutase (SOD), peroxidase (POX), ascorbate peroxidase (APOX), catalase (CAT) and glutathione reductase (GR) were inherently higher than in B. vulgaris cv. ansa . Compared to B. vulgaris , lipid peroxidation was also lower and the activities of SOD, POX, APOX, CAT and GR were higher in B. maritima at 150 and 500 mM NaCl. These results possibly suggest that the wild salt-tolerant beet, B. maritima exhibit a better protection mechanism against oxidative damage by maintaining a higher inherited and induced activity of antioxidant enzymes than the relatively sensitive plants of the sugar beet, B. vulgaris cv. ansa .

Effects of 24-epibrassinolide on seed germination, seedling growth, lipid peroxidation, proline content and antioxidative system of rice (Oryza sativa L.) under salinity stress

The effects of 24-epibrassinolide (24-epiBL) on seedling growth, antioxidative system, lipid peroxidation, proline and soluble protein content were investigated in seedlings of the salt-sensitive rice cultivar IR-28. Seedling growth of rice plants was improved by 24-epiBL treatment under salt stress conditions. When seedlings treated with 24-epiBL were subjected to 120 mM NaCl stress, the activities of superoxide dismutase (EC 1.15.1.1), catalase (EC 1.11.1.6) and glutathione reductase (EC 1.6.4.2) did not show significant difference, whereas the activity of ascorbate peroxidase (EC 1.11.1.11) significantly increased. Increased activity of peroxidase (EC 1.11.1.7) under NaCl stress showed remarkable decrease in the 24-epiBL+NaCl-applied group. Lipid peroxidation level significantly increased under salt stress but decreased with 24-epiBL application revealing that less oxidative damage occurred in this group (24-epiBL+NaCl). In addition, increased proline content in the NaCl-applied group was decreased by 24-epiBL application in the 24-epiBL+NaCl-applied group. Soluble protein content was increased by 24-epiBL application even under NaCl stress, being also higher than control conditions (no 24-epiBL or NaCl treatment). 24-epiBL treatment considerably alleviated oxidative damage that occurred under NaCl-stressed conditions and improved seedling growth in part under salt stress in sensitive IR-28 seedlings.

Contribution of Gamma amino butyric acid (GABA) to salt stress responses of Nicotiana sylvestris CMSII mutant and wild type plants.

Plants accumulate high levels of Gamma amino butyric acid (GABA) in response to different environmental stresses and GABA metabolism has different functions such as osmotic and pH regulation, bypass of tricarboxylic acid cycle, and C:N balance. The cytoplasmic male sterile (CMS) II mutant of Nicotiana sylvestris has a deletion in the mitochondrial gene nad7 which encodes the NAD7 subunit of complex I which causes increased leaf respiration, impaired photosynthesis, slower growth and increased amino acid levels. In this study we aimed to elucidate the role of GABA and GABA metabolism in different genotypes of the same plant system under salt stress (100mM NaCl) in short (24h) and long (7, 14 and 21 days) terms. We have investigated the differences in leaf fresh and dry weights, relative water content, photosynthetic efficiency (F(v)/F(m)), glutamate dehydrogenase (GDH, EC 1.4.1.4) and glutamate decarboxylase (GAD, EC 4.1.1.15) enzyme activities, GABA content and GAD gene expression profiles. GDH activity showed variations in CMSII and wild type (WT) plants in the first 24h. GAD gene expression profiles were in good agreement with the GAD enzyme activity levels in CMSII and WT plants after 24h. In long-term salinity, GAD activities increased in WT but, decreased in CMSII. GABA accumulation in WT and CMSII plants in short and long term was induced by salt stress. Variations in GDH and GAD activities in relation to GABA levels were discussed and GABA metabolism has been proposed to be involved in better performance of CMSII plants under long term salinity.

Glycine betaine protects tomato (Solanum lycopersicum) plants at low temperature by inducing fatty acid desaturase7 and lipoxygenase gene expression.

Cold stress is among the environmental stressors limiting productivity, yield and quality of agricultural plants. Tolerance to cold stress is associated with the increased unsaturated fatty acids ratio in the plant membranes which are also known to be substrates of octadecanoid pathway for jasmonate and other oxylipins biosynthesis. Accumulation of osmoprotectant, glycine betaine (GB) is well known to be effective in the protecting membranes and mitigating cold stress effects but, the mode of action is poorly understood. We studied the role of GB in cold stress responses of two tomato cultivated varieties; Gerry (cold stress sensitive) and T47657 (moderately cold stress tolerant) and compared the differences in lypoxygenase-13 (TomLOXF) and fatty acid desaturase 7 (FAD7) gene expression profiles and physiological parameters including relative growth rates, relative water content, osmotic potential, photosynthetic efficiency, membrane leakage, lipid peroxidation levels. Our results indicated that GB might have a role in inducing FAD7 and LOX expressions for providing protection against cold stress in tomato plants which could be related to the desaturation process of lipids leading to increased membrane stability and/or induction of other genes related to stress defense mechanisms via octadecanoid pathway or lipid peroxidation products.

Arabidopsis NATA1 acetylates putrescine and decreases defense-related hydrogen peroxide accumulation

Putrescine acetylation in Arabidopsis thaliana reduces production of hydrogen peroxide by polyamine oxidases, thereby promoting plant susceptibility to Pseudomonas syringae. Biosynthesis of the polyamines putrescine, spermidine, and spermine is induced in response to pathogen infection of plants. Putrescine, which is produced from Arg, serves as a metabolic precursor for longer polyamines, including spermidine and spermine. Polyamine acetylation, which has important regulatory functions in mammalian cells, has been observed in several plant species. Here we show that Arabidopsis (Arabidopsis thaliana) N-ACETYLTRANSFERASE ACTIVITY1 (NATA1) catalyzes acetylation of putrescine to N-acetylputrescine and thereby competes with spermidine synthase for a common substrate. NATA1 expression is strongly induced by the plant defense signaling molecule jasmonic acid and coronatine, an effector molecule produced by DC3000, a Pseudomonas syringae strain that initiates a virulent infection in Arabidopsis ecotype Columbia-0. DC3000 growth is reduced in nata1 mutant Arabidopsis, suggesting a role for NATA1-mediated putrescine acetylation in suppressing antimicrobial defenses. During infection by P. syringae and other plant pathogens, polyamine oxidases use spermidine and spermine as substrates for the production of defense-related H2O2. Compared to wild-type Columbia-0 Arabidopsis, the response of nata1mutants to P. syringae infection includes reduced accumulation of acetylputrescine, greater abundance of nonacetylated polyamines, elevated H2O2 production by polyamine oxidases, and higher expression of genes related to pathogen defense. Together, these results are consistent with a model whereby P. syringae growth is improved in a targeted manner through coronatine-induced putrescine acetylation by NATA1.

The impact of GABA in harpin-elicited biotic stress responses in Nicotiana tabaccum

Harpin is a bacterial elicitor protein that was first isolated from Erwinia amylovora. Infiltration of this elicitor into the leaves of plants activates systemic acquired resistance against a variety of plant pathogens via the salicyclic acid defense pathway. The non-protein amino acid, neurotransmission inhibitor molecule of mammals-GABA- is found in all organisms and is known to be an important component of stress responses in plants. We hypothesized a possible interaction between harpin-induced defense responses and GABA shunt. Therefore, we conducted experiments on harpin-infiltrated tobacco and analyzed the components of GABA shunt in relation to growth, photosynthesis and H2O2 levels. RGR, RWC and photosynthetic efficiency were all affected in harpin-infiltrated tobacco leaves, but the rate of decline was more remarkable on RGR. H2O2 levels showed significant difference on 7 days after harpin infiltration when the necrotic lesions were also visible. GABA accumulation was increased and glutamate levels were decreased parallel to the differences in GDH and GAD enzyme activities, especially on days 5 and 7 of harpin infiltration. Transcript abundance of GDH and GAD encoding genes were differentially regulated in harpin-infiltrated leaves as compared to that of control and mock groups. In the present study, for the first time we showed a relationship between harpin-elicited responses and GABA in tobacco that was not mediated by H2O2 accumulation. Harpin infiltration significantly induced the first components of the GABA shunt such as GDH, GAD, glutamate and GABA in tobacco.

Ozone triggers different defence mechanisms against powdery mildew (Blumeria graminis DC. Speer f. sp. tritici) in susceptible and resistant wheat genotypes

Ozone has been proposed as a convenient elicitor against pathogens since it is known to generate different reactive oxygen species (ROS) and induce nonspecific defence by altering gene expression. The mode of action and its interaction with other defence pathways are yet to be elucidated. Besides its negative effects on plants, ozone can be used for triggering defence against environmental stresses, including pathogens, when used at appropriate concentrations. Powdery mildew, caused by the obligate biotrophic fungus Blumera graminis f.sp. tritici (Bgt), is an important plant disease that reduces crop yield and quality. We hypothesised that ozone treatment may elicit defence against Bgt by inducing ROS signalling or other routes such as the salicylic acid (SA) or jasmonic acid (JA) pathways. We conducted experiments with Bgt-susceptible (cv. Pamukova) and resistant (cv. Tahirova) wheat (Triticum aestivum L,) cultivars and treated them with different ozone concentrations before Bgt inoculation. Stress response and defence-related features such as antioxidative enzyme activity; lipid peroxidation; H2O2 and Ca+2 levels; PR1, LOX, PAL and RBOH gene expression; and disease severity were assayed. Clear discrepancies between the responses of susceptible and resistant cultivars were found, suggesting that different defence routes were activated. Here, we showed that ozone treatment was effective for diminishing Bgt invasion in the susceptible cultivar in the short term, which was probably related to defence induced via the SA pathway. Moreover, the resistant cultivar Tahirova exhibited a different mode of action against the pathogen that was triggered by ozone treatment, plausibly related to the JA pathway.

Manipulating Metabolic Pathways for Development of Salt-Tolerant Crops

Engineering plants for salt stress tolerance is a complex process due to the multiple-sided characteristics of stress coping mechanisms. The common approach was first identification of the components of signalling and regulatory pathways for salinity tolerance, then transformation of plants with one of those genes and phenotyping the transgenic plant subjected to salt stress at controlled conditions. Plant biology literature is full of research papers on the success of such plants to acclimate and survive under salinity; however, to date none of them was able to become a commercial variety having improved performance at field conditions. Disturbing or interfering with complex networks and pathways can result in unexpected effects on plant growth and development. Furthermore, tolerance against one stress would not be efficient to cope with different environmental stress factors which field-grown plants encounter during a single growth season.