Melinda A. McFarland
Florida State University
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Featured researches published by Melinda A. McFarland.
Analytical Chemistry | 2014
Melinda A. McFarland; Denis Andrzejewski; Steven M. Musser; John H. Callahan
Intact protein expression profiling has proven to be a powerful tool for bacterial subspecies differentiation. To facilitate typing, epidemiology, and trace-back of Salmonella contamination in the food supply, a minimum of serovar level differentiation is required. Subsequent identification and validation of marker proteins is integral to rapid screening development and to determining which proteins are subject to environmental pressure. Bacterial sequencing efforts have expanded the number of sequenced genomes available for single-nucleotide polymorphism (SNP) analyses, but annotation is often missing, start site errors are not uncommon, and the likelihood of expression is not known. In this work we show that the combination of intact protein expression profiles and top-down liquid chromatography-mass spectrometry (LC-MS/MS) facilitates the identification of proteins that result from expressed serovar specific nonsynonymous SNPs. Combinations of these marker proteins can be used in assays for rapid differentiation of bacteria. LC-MS generated intact protein expression profiles establish which bacterial protein masses differ across samples and can be determined without prior knowledge of the sample. Subsequent top-down LC-MS/MS is used to identify expressed proteins and their post-translational modifications (PTM), identify serovar specific markers, and validate genomic predicted orthologues as expressed biomarkers.
European Journal of Mass Spectrometry | 2004
Ulf Mazurek; Melinda A. McFarland; Alan G. Marshall; Chava Lifshitz
A method to isolate isomers in a Fourier transform ion cyclotron resonance mass spectrometer on the basis of their different hydrogen/deuterium exchange rates has been developed and applied to the protonated serine octamer cluster. Isolation allows interrogation of each isomer by infrared multi-photon dissociation to determine any differences in the building blocks that form these clusters. The experimental results strongly support previous assignments of an all-zwitterion structure to the slower-exchanging isomer and an all-neutral structure to its faster-exchanging counterpart.
Analytical Chemistry | 2003
Kristina Håkansson; Michael J. Chalmers; John P. Quinn; Melinda A. McFarland; Christopher L. Hendrickson; Alan G. Marshall
Journal of the American Society for Mass Spectrometry | 2005
Melinda A. McFarland; Alan G. Marshall; Christopher L. Hendrickson; Carol L. Nilsson; Pam Fredman; Jan-Eric Månsson
Journal of the American Society for Mass Spectrometry | 2005
Melinda A. McFarland; Michael J. Chalmers; John P. Quinn; Christopher L. Hendrickson; Alan G. Marshall
Journal of Physical Chemistry A | 2005
Ulf Mazurek; Orit Geller; Chava Lifshitz; Melinda A. McFarland; Alan G. Marshall; Bryan G. Reuben
Chemical Physics Letters | 2003
Bryan G. Reuben; Ya'acov Ritov; Orit Geller; Melinda A. McFarland; Alan G. Marshall; Chava Lifshitz
Journal of Physical Chemistry A | 2002
Brant Cage; Melinda A. McFarland; Christopher L. Hendrickson; N. S. Dalal; Alan G. Marshall
Chemical Physics Letters | 2004
Brant Cage; Jochen Friedrich; Reginald B. Little; Yi-Sheng Wang; Melinda A. McFarland; Christopher L. Hendrickson; N. S. Dalal; Alan G. Marshall
Analytical Chemistry | 2004
Melinda A. McFarland; Christopher L. Hendrickson; Alan G. Marshall