Melissa J. Lafrenière

Diversity, Abundance, and Potential Activity of Nitrifying and Nitrate-Reducing Microbial Assemblages in a Subglacial Ecosystem

ABSTRACT Subglacial sediments sampled from beneath Robertson Glacier (RG), Alberta, Canada, were shown to harbor diverse assemblages of potential nitrifiers, nitrate reducers, and diazotrophs, as assessed by amoA, narG, and nifH gene biomarker diversity. Although archaeal amoA genes were detected, they were less abundant and less diverse than bacterial amoA, suggesting that bacteria are the predominant nitrifiers in RG sediments. Maximum nitrification and nitrate reduction rates in microcosms incubated at 4°C were 280 and 18.5 nmol of N per g of dry weight sediment per day, respectively, indicating the potential for these processes to occur in situ. Geochemical analyses of subglacial sediment pore waters and bulk subglacial meltwaters revealed low concentrations of inorganic and organic nitrogen compounds. These data, when coupled with a C/N atomic ratio of dissolved organic matter in subglacial pore waters of ∼210, indicate that the sediment communities are N limited. This may reflect the combined biological activities of organic N mineralization, nitrification, and nitrate reduction. Despite evidence of N limitation and the detection of nifH, we were unable to detect biological nitrogen fixation activity in subglacial sediments. Collectively, the results presented here suggest a role for nitrification and nitrate reduction in sustaining microbial life in subglacial environments. Considering that ice currently covers 11% of the terrestrial landmass and has covered significantly greater portions of Earth at times in the past, the demonstration of nitrification and nitrate reduction in subglacial environments furthers our understanding of the potential for these environments to contribute to global biogeochemical cycles on glacial-interglacial timescales.

Fluvial Impact of Extensive Active Layer Detachments/ Cape Bounty, Melville Island, Canada

Abstract Exceptional and persistent warm temperatures recorded during July 2007 at Cape Bounty, Melville Island, Canada (74°54′N, 109°35′W), resulted in rapid and deep active layer formation. The thickened active layer, together with up to 10.8 mm of rainfall in late July, resulted in widespread active layer detachments across the West watershed during 23–31 July. Mapping indicates that approximately 1.9% of the watershed was directly impacted by disturbances. By contrast, only two small detachments occurred in the adjacent East watershed. The immediate fluvial impact of the detachments was primarily in the form of abrupt, short-lived rises in river turbidity, along with a more gradual increase in discharge and overall turbidity. Sediment transport pulses resulted from the hydrological connection of major detachment slides, most of which were upslope from the main channel. The largest detachment dammed the river over a length of 200 m, and resulted in an upstream pond and prolonged increased sediment transport. In total, the increased sediment transport during the last week of July amounted to an estimated 44.3 Mg, or 18% of the seasonal yield. While the detachments had an immediate and substantial impact on river conditions, erosion of unstable material is likely to have a sustained impact on watershed fluxes in future years.

Influence of bedrock mineral composition on microbial diversity in a subglacial environment

Microorganisms in subglacial environments drive the chemical weathering of bedrock; however, the influence of bedrock mineralogy on the composition and activity of microbial assemblages in such environments is poorly understood. Here, using a combination of in situ mineral incubation and DNA fingerprinting techniques, we demonstrate that pyrite is the dominant mineralogical control on subglacial bacterial community structure and composition. In addition, we show that the abundance of Fe in the incubated minerals influences the development of mineral-associated biomass. The ubiquitous nature of pyrite in many common bedrock types and high SO 4 2– concentrations in most glacial meltwaters suggest that pyrite may be a dominant lithogenic control on microbial communities in many subglacial systems. Mineral-based energy may therefore serve a fundamental role in sustaining subglacial microbial populations and enabling their persistence over glacial-interglacial time scales.

Seasonal fluxes and age of particulate organic carbon exported from Arctic catchments impacted by localized permafrost slope disturbances

Projected warming is expected to alter the Arctic permafrost regime with potential impacts on hydrological fluxes of particulate organic carbon (POC) and sediment. Previous work has focused on large Arctic basins and revealed the important contribution of old carbon in river POC, but little is known about POC fluxes from smaller coastal watersheds, particularly where widespread postglacial raised marine sediments represent a potential source of old soil carbon that could be mobilized by permafrost disturbance. To evaluate these processes, the characteristics of POC, particulate nitrogen (PN) and suspended sediment transport from paired small coastal Arctic watersheds subject to recent permafrost disturbance were investigated at the Cape Bounty Arctic Watershed Observatory (CBAWO) in the Canadian High Arctic. Approximately 2% of the total suspended sediment load from both watersheds was composed of POC and the majority of the sediment and POC fluxes occurred during the spring snowmelt period. Radiocarbon analysis of POC indicates recent permafrost disturbances deliver substantially older POC to the aquatic system. Localized permafrost slope disturbances have a measurable influence on downstream POC age and dominate (estimated up to 78% of POC) sediment fluxes during summer baseflow. The elevation of disturbances and Holocene emergence data show limited age sensitivity of POC to the location of disturbance and suggest slope failures are likely to deliver carbon with a relatively similar age range to the aquatic system, regardless of landscape location.

Stable isotopic evidence of enhanced export of microbially derived \({\text{NO}}_{3}^{ - }\) following active layer slope disturbance in the Canadian High Arctic

Permafrost disturbance is expected to alter nitrogen (N) export in High Arctic watersheds by enhancing loads of dissolved inorganic N (DIN), particularly nitrate (NO3-\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}

Evaluating the hydrological and hydrochemical responses of a High Arctic catchment during an exceptionally warm summer

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Climate and permafrost effects on the chemistry and ecosystems of High Arctic Lakes

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