Mervi Sepp

ADP Compartmentation Analysis Reveals Coupling between Pyruvate Kinase and ATPases in Heart Muscle

Abstract Cardiomyocytes have intracellular diffusion restrictions, which spatially compartmentalize ADP and ATP. However, the models that predict diffusion restrictions have used data sets generated in rat heart permeabilized fibers, where diffusion distances may be heterogeneous. This is avoided by using isolated, permeabilized cardiomyocytes. The aim of this work was to analyze the intracellular diffusion of ATP and ADP in rat permeabilized cardiomyocytes. To do this, we measured respiration rate, ATPase rate, and ADP concentration in the surrounding solution. The data were analyzed using mathematical models that reflect different levels of cell compartmentalization. In agreement with previous studies, we found significant diffusion restriction by the mitochondrial outer membrane and confirmed a functional coupling between mitochondria and a fraction of ATPases in the cell. In addition, our experimental data show that considerable activity of endogenous pyruvate kinase (PK) remains in the cardiomyocytes after permeabilization. A fraction of ATPases were inactive without ATP feedback by this endogenous PK. When analyzing the data, we were able to reproduce the measurements only with the mathematical models that include a tight coupling between the fraction of endogenous PK and ATPases. To our knowledge, this is the first time such a strong coupling of PK to ATPases has been demonstrated in permeabilized cardiomyocytes.

Unchanged mitochondrial organization and compartmentation of high-energy phosphates in creatine-deficient GAMT-/- mouse hearts.

Disruption of the creatine kinase (CK) system in hearts of CK-deficient mice leads to changes in the ultrastructure and regulation of mitochondrial respiration. We expected to see similar changes in creatine-deficient mice, which lack the enzyme guanidinoacetate methyltransferase (GAMT) to produce creatine. The aim of this study was to characterize the changes in cardiomyocyte mitochondrial organization, regulation of respiration, and intracellular compartmentation associated with GAMT deficiency. Three-dimensional mitochondrial organization was assessed by confocal microscopy. On populations of permeabilized cardiomyocytes, we recorded ADP and ATP kinetics of respiration, competition between mitochondria and pyruvate kinase for ADP produced by ATPases, ADP kinetics of endogenous pyruvate kinase, and ATP kinetics of ATPases. These data were analyzed by mathematical models to estimate intracellular compartmentation. Quantitative analysis of morphological and kinetic data as well as derived model fits showed no difference between GAMT-deficient and wild-type mice. We conclude that inactivation of the CK system by GAMT deficiency does not alter mitochondrial organization and intracellular compartmentation in relaxed cardiomyocytes. Thus, our results suggest that the healthy heart is able to preserve cardiac function at a basal level in the absence of CK-facilitated energy transfer without compromising intracellular organization and the regulation of mitochondrial energy homeostasis. This raises questions on the importance of the CK system as a spatial energy buffer in unstressed cardiomyocytes.

Permeabilized Rat Cardiomyocyte Response Demonstrates Intracellular Origin of Diffusion Obstacles

Intracellular diffusion restrictions for ADP and other molecules have been predicted earlier based on experiments on permeabilized fibers or cardiomyocytes. However, it is possible that the effective diffusion distance is larger than the cell dimensions due to clumping of cells and incomplete separation of cells in fiber preparations. The aim of this work was to check whether diffusion restrictions exist inside rat cardiomyocytes or are caused by large effective diffusion distance. For that, we determined the response of oxidative phosphorylation (OxPhos) to exogenous ADP and ATP stimulation in permeabilized rat cardiomyocytes using fluorescence microscopy. The state of OxPhos was monitored via NADH and flavoprotein autofluorescence. By varying the ADP or ATP concentration in flow chamber, we determined that OxPhos has a low affinity in cardiomyocytes. The experiments were repeated in a fluorometer on cardiomyocyte suspensions leading to similar autofluorescence changes induced by ADP as recorded under the microscope. ATP stimulated OxPhos more in a fluorometer than under the microscope, which was attributed to accumulation of ADP in fluorometer chamber. By calculating the flow profile around the cell in the microscope chamber and comparing model solutions to measured data, we demonstrate that intracellular structures impose significant diffusion obstacles in rat cardiomyocytes.

Tight coupling of Na+/K+-ATPase with glycolysis demonstrated in permeabilized rat cardiomyocytes.

The effective integrated organization of processes in cardiac cells is achieved, in part, by the functional compartmentation of energy transfer processes. Earlier, using permeabilized cardiomyocytes, we demonstrated the existence of tight coupling between some of cardiomyocyte ATPases and glycolysis in rat. In this work, we studied contribution of two membrane ATPases and whether they are coupled to glycolysis - sarcoplasmic reticulum Ca2+ ATPase (SERCA) and plasmalemma Na+/K+-ATPase (NKA). While SERCA activity was minor in this preparation in the absence of calcium, major role of NKA was revealed accounting to ∼30% of the total ATPase activity which demonstrates that permeabilized cell preparation can be used to study this pump. To elucidate the contribution of NKA in the pool of ATPases, a series of kinetic measurements was performed in cells where NKA had been inhibited by 2 mM ouabain. In these cells, we recorded: ADP- and ATP-kinetics of respiration, competition for ADP between mitochondria and pyruvate kinase (PK), ADP-kinetics of endogenous PK, and ATP-kinetics of total ATPases. The experimental data was analyzed using a series of mathematical models with varying compartmentation levels. The results show that NKA is tightly coupled to glycolysis with undetectable flux of ATP between mitochondria and NKA. Such tight coupling of NKA to PK is in line with its increased importance in the pathological states of the heart when the substrate preference shifts to glucose.

Metabolic compartmentation in rainbow trout cardiomyocytes: coupling of hexokinase but not creatine kinase to mitochondrial respiration

Rainbow trout (Oncorhynchus mykiss) cardiomyocytes have a simple morphology with fewer membrane structures such as sarcoplasmic reticulum and t-tubules penetrating the cytosol. Despite this, intracellular ADP diffusion is restricted. Intriguingly, although diffusion is restricted, trout cardiomyocytes seem to lack the coupling between mitochondrial creatine kinase (CK) and respiration. Our aim was to study the distribution of diffusion restrictions in permeabilized trout cardiomyocytes and verify the role of CK. We found a high activity of hexokinase (HK), which led us to reassess the situation in trout cardiomyocytes. We show that diffusion restrictions are more prominent than previously thought. In the presence of a competitive ADP-trapping system, ADP produced by HK, but not CK, was channeled to the mitochondria. In agreement with this, we found no positively charged mitochondrial CK in trout heart homogenate. The results were best fit by a simple mathematical model suggesting that trout cardiomyocytes lack a functional coupling between ATPases and pyruvate kinase. The model simulations show that diffusion is restricted to almost the same extent in the cytosol and by the outer mitochondrial membrane. Furthermore, they confirm that HK, but not CK, is functionally coupled to respiration. In perspective, our results suggest that across a range of species, cardiomyocyte morphology and metabolism go hand in hand with cardiac performance, which is adapted to the circumstances. Mitochondrial CK is coupled to respiration in adult mammalian hearts, which are specialized to high, sustained performance. HK associates with mitochondria in hearts of trout and neonatal mammals, which are more hypoxia-tolerant.

Life of mice – development of cardiac energetics

Production and transfer of metabolites like ATP and phosphocreatine within cardiomyocytes is crucial for the robust availability of mechanical work. In mammalian cardiomyocytes, mitochondria, the main suppliers of usable chemical energy in the form of ATP, are situated adjacent to both the ATPases near the mechanical apparatus, and the sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) calcium pumps. Operation of these ATPases requires a high ATP/ADP ratio, which is maintained by two parallel energy transfer systems – creatine kinase (CK) and direct adenine nucleotide channelling (DANC). Compartmentation of energy metabolites works to lessen the impact of dynamic changes in the availability of usable energy on the operation of these ATPases, and allows for a higher phosphorylation potential where it is required most. The operational mechanism, structure and development of the barriers responsible for energetic compartmentation within cardiomyocytes have yet to be elucidated despite intensive research in this area. A recent article in The Journal of Physiology by Piquereau et al. (2010) is an extensive investigation into how the structural and energetic properties of mouse heart muscle change during postnatal development. It includes observations on structural changes and cellular morphology using electron microscopy, quantification of mitochondrial, myofibrillar and SR proteins, assessment of organelle functionality, and the quantification of the energy flux in both the CK and DANC transfer systems. SERCA function was measured via calcium mediated tension generation, while myosin ATPase function was quantified by measuring rigor tension development. Total activity of CK and mitochondrial CK (mi-CK) were estimated. The article by Piquereau et al. builds upon a strong research tradition at Inserm U769, Univ. Paris-Sud, which focuses on studying how cardiac mechanisms function in response to both pathological and physiological stimuli. This includes work on contractile, sarcoplasmic reticulum (SR), and mitochondrial proteins, membrane receptors, ion channels and signalling. Their work has inspired new areas of inquiry into the function of energy compartmentation in the heart with various implications for therapeutic targets to improve both function and clinical outcomes. As main results of their recent publication, Piquereau et al. concluded that the formation of energetic microdomains occurs very early in postnatal development, and that the maturation of cellular architecture plays an important role in achieving maximal flexibility in regulation of ATP production by mitochondria. They found that the development of regulatory energetic pathways does not happen simultaneously. Throughput of energy transfer between mitochondria and myosin ATPases is correlated with the changes in the cytoarchitecture in contrast to the CK supported energy transfer which seems to depend on specific localization and expression of CK. Development between days 3 and 7 is crucial in increasing the capacity of energy transfer and involves major remodelling of the contacts between organelles. The density of intracellular organelles increases at the expense of free cytosolic space. Contacts between mitochondria and longitudinally oriented myofibrils and between SR and mitochondria are established to form an effective intracellular energetic unit. After the first week (post natum), a different phase of hypertrophy occurs without major structural changes to the contacts between organelles. After 3 weeks, the respiratory capacity of mitochondria increases, whereas heart weight to body weight ratio decreases. The main results of the article are summarized in Fig. 1. Figure 1 Summary of results from the article by Piquereau et al. Considerable effort has been invested by Piquereau et al. in determining various changes during cardiomyocyte maturation. Several questions arise, however, when comparing the publication with previous studies. Firstly, in 3-day-old cells, based on results from electron microscopy and SR protein expression experiments, the authors deduce SR not to be present in quantities high enough to enable SR Ca2+ content measurement. However, volume measurements from electron microscopy are known to be very sensitive to sample preparation procedures, especially as dimensions of different organelles can change in different ratios as a result of fixation. The low level of SR protein expression in 3-day-old cells could be explained by results obtained in embryonic mouse cardiomyocytes (Takeshima et al. 1998), where SR Ca2+ release channels do not play a major role in excitation–contraction (EC) coupling but, instead, are required for cellular Ca2+ homoestasis. Full SR function develops rapidly in neonates, possibly explaining both the dramatic increase in SR Ca2+ content between day 3 and day 7 fibres, and the difficulty the authors had in conducting the experiment with fibres from 3-day-old mice. Secondly, the authors concluded that the functional coupling of adenine nucleotide translocase (ANT) and mi-CK (‘functional activity’ in Piquereau et al. 2010) was considerably higher in adult myocytes. This conclusion, however, seems to be based on misinterpreting the KmADP/KCr ratio graph (article Fig. 5F). As is evident from the Km plots in the article (article Fig. 5E), KmCr is constant throughout the ageing process, whereas KmADP increases notably in older fibres. The increase in KmADP/KCr ratio stems from the increase of KmADP and is not, in this case, indicating increases in mi-CK–ANT coupling nor mi-CK activity. Rather, it can be interpreted as indication of an increase in diffusion restrictions to adenine nucleotides in the cytosol caused by changes in either mitochondrial outer membrane or myofibrillar and other cytosolic structures, or both (Vendelin & Birkedal, 2008; Sepp et al. 2010). In order to measure the coupling between mi-CK and ANT, different experimental techniques need to be employed, such as measuring changes in respiration in response to ATP titration. Two observations can be made from further analysing SR calcium uptake and rigor tension sensitivity results from the article (article Figs 2 and 4). By looking at ratios of values obtained during different conditions, it is possible to eliminate auxiliary effects and focus on how the role of energy supply pathways change in relation to one another as the cell matures. Two examples are given in Fig. 1 (bottom row). Firstly, from the difference in rigor tension levels (ΔpMgATP50) supported by CK and ATP energy supply systems (Fig. 1, line b), it is evident that myosin ATPase activity supported by CK is consistently higher than exogenous ATP throughout the growing process. On the other hand, the capacity of the CK system to load the SR increases ∼2 times by day 61 (Fig. 1, line d). We suggest that this is further evidence of the role of SR transitioning from maintaining Ca2+ homoestasis (Takeshima et al. 1998) to playing an essential role in EC coupling. A possible explanation for this could be activation of SR-bound CK by day 21, whereas myofibril bound CK is already active from day three. Secondly, pMgATP50(DANC) – pMgATP50(ATP) (Fig. 1, line a) indicates that after an initial increase caused by changes in mitochondrial positioning, myofibrils stay constantly more sensitive to stimulation via direct channelling compared to exogenous ATP. At the same time, however, direct channelling is able to maintain an increasingly higher SR load than exogenous ATP (Fig. 1, line c). This can be explained by structural changes in the cell, whereby SR becomes more closely situated with respect to mitochondria (article Fig. 8D). Clearly, these interpretations should be verified through further experiments and modelling. Building on results obtained by Piquereau et al. some directions could be explored in the future. One matter of interest would be how the role of glycolysis changes during maturation. It has been shown that embryonic mouse heart responds in a similar manner to inhibition of either glycolysis or oxidative phosphorylation and that in early stages of postnatal development, ATP consumed by ion pumps is preferentially supplied through glycolysis (Chen et al. 2007). Additionally, in 1-day-old rabbit, 44% of consumed ATP comes from glycolysis, whereas by day 7 this goes down to 7% (Lopaschuk et al. 1992). In the paper under discussion, the possible contribution of glycolysis to ATP supply was not directly addressed. Especially in young mouse cells, the effect from this could be considerable and might impact some of the conclusions of the article. Another possible area to explore in the future could be to analyse these results with the aid of a computational model. This would help in further unravelling the interplay between different factors during cell maturation, especially in questions where experimental methods fail to yield clear results. Different mathematical models could be compared with statistical methods in order to determine the role of various pathways and the existence of metabolite pools or spatial compartmentation in the developing cell (Sepp et al. 2010). In summary, the extensive experimental work performed in the work by Piquereau et al. covers various aspects of energy metabolism and morphological changes in the cell during maturation. The work provides new information on postnatal development of heart energetics in mice – a popular animal model used for studying the effects of genetic manipulation.