Mervyn O. Humphreys
Aberystwyth University
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Featured researches published by Mervyn O. Humphreys.
The Journal of Agricultural Science | 2003
P W Wilkins; Mervyn O. Humphreys
Wilkins, P. W., Humphreys, M. O. (2003). Progress in breeding perennial forage grasses for temperate agriculture. Journal of Agricultural Science, 140, (2), 129-150. Sponsorship: Germinal Holdings Ltd; Department for Agriculture, Food and Rural Affairs (DEFRA); Biotechnology and Biological Sciences Research Council (BBSRC)
Molecular Breeding | 2005
Leif Skøt; Mervyn O. Humphreys; Ian P. Armstead; Susan Heywood; Kirsten P. Skøt; Ruth Sanderson; I. D. Thomas; Ken H. Chorlton; N. Ruaraidh Sackville Hamilton
We describe an association mapping approach using natural populations of perennial ryegrass (Lolium perenne L.) to identify molecular markers associated with heading date, an important trait affecting seasonal production, tillering, digestibility and grassland management regimes. Twenty-three natural populations originating from throughout Europe, with heading date phenotypes ranging from very early to very late, as well as three synthetic populations (varieties) were used for molecular marker genotyping using AFLP. In total, 589 polymorphic markers were identified. Hierarchical clustering, principal coordinate and other statistical analyses identified four outlying populations forming a clearly distinct sub-group. Removal of those four populations from the subsequent analysis reduced population sub-structure twofold. However, this made relatively little difference to the result of the association analysis. Linear regression identified three markers whose frequency of occurrence correlated with the heading date phenotype. Moreover, these markers were shown to be closely linked to each other within a major QTL on Chromosome 7, explaining 70% of the total variation in heading date. Pairwise linkage disequilibrium among them was also significant. These results suggest that association mapping approaches may be feasible in L. perenne, and that the use of natural populations could provide a useful source of genetic variation in traits of importance in crop improvement.
Theoretical and Applied Genetics | 2004
Ian P. Armstead; Lesley B. Turner; Markku S. Farrell; Leif Skøt; P. Gomez; Teresa Montoya; Iain S. Donnison; I. P. King; Mervyn O. Humphreys
The genetic control of induction to flowering has been studied extensively in both model and crop species because of its fundamental biological and economic significance. An ultimate aim of many of these studies has been the application of the understanding of control of flowering that can be gained from the study of model species, to the improvement of crop species. The present study identifies a region of genetic synteny between rice and Lolium perenne, which contains the Hd3 heading-date QTL in rice and a major QTL, accounting for up to 70% of the variance associated with heading date in L. perenne. The identification of synteny between rice and L. perenne in this region demonstrates the direct applicability of the rice genome to the understanding of biological processes in other species. Specifically, this syntenic relationship will greatly facilitate the genetic dissection of aspects of heading-date induction by enabling the magnitude of the genetic component of the heading-date QTL in L. perenne to be combined with the sequencing and annotation information from the rice genome.
The Journal of Agricultural Science | 1991
Henry Thomas; Mervyn O. Humphreys
The Lolium-Festuca complex of related species provides a wide range of variation for the development of versatile grasses adapted to meet the changing requirements of livestock farming. Various approaches have been used in order to harness the full potential of this variation. Tetraploid hybrids between the two ryegrass species L. perenne and L. multiflorum have resulted in stable and successful commercial varieties. Hybrids between more distantly related ryegrass and fescue species have demonstrated useful combinations of traits from both parents. Chromosome doubling is necessary in these hybrids to restore fertility in synthetic amphiploids. However, genetic instability can still be a problem and research is in progress to obtain tighter control of chromosome pairing. An alternative approach is to introduce a few specific traits from fescues into ryegrasses, or vice versa, using introgressive breeding procedures. This review outlines the progress already achieved in exploiting the ryegrass–fescue complex by using these various approaches and assesses the potential of hybrid derivatives.
Genetics | 2007
Leif Skøt; Janet Humphreys; Mervyn O. Humphreys; Daniel Thorogood; Joseph Gallagher; Ruth Sanderson; Ian P. Armstead; I. D. Thomas
We describe a candidate gene approach for associating SNPs with variation in flowering time and water-soluble carbohydrate (WSC) content and other quality traits in the temperate forage grass species Lolium perenne. Three analysis methods were used, which took the significant population structure into account. First, a linear mixed model was used enabling a structured association analysis to be incorporated with the nine populations identified in the structure analysis as random variables. Second, a within-population analysis of variance was performed. Third, a tree-scanning method was used, in which haplotype trees were associated with phenotypes on the basis of inferred haplotypes. Analysis of variance within populations identified several associations between WSC, nitrogen (N), and dry matter digestibility with allelic variants within an alkaline invertase candidate gene LpcAI. These associations were only detected in material harvested in one of the two years. By contrast, consistent associations between the L. perenne homolog (LpHD1) of the rice photoperiod control gene HD1 and flowering time were identified. One SNP, in the immediate upstream region of the LpHD1 coding sequence (C-4443-A), was significant in the linear mixed model. Within-population analysis of variance and tree-scanning analysis confirmed and extended this result to the 2118 polymorphisms in some of the populations. The merits of the tree-scanning method are compared to the single SNP analysis. The potential usefulness of the 4443 SNP in marker-assisted selection is currently being evaluated in test crosses of genotypes from this work with turf-grass varieties.
New Phytologist | 2008
Hiroshi Hisano; Akira Kanazawa; Midori Yoshida; Mervyn O. Humphreys; Masaru Iizuka; Keisuke Kitamura; Toshihiko Yamada
* Fructan is the major nonstructural carbohydrate reserve in temperate grasses. To understand regulatory mechanisms in fructan synthesis and adaptation to cold environments, the isolation, functional characterization and genetic mapping of fructosyltransferase (FT) genes in perennial ryegrass (Lolium perenne) are described. * Six cDNAs (prft1-prft6) encoding FTs were isolated from cold-treated ryegrass plants, and three were positioned on a perennial ryegrass linkage map. Recombinant proteins were produced in Pichia pastoris and enzymatic activity was characterized. Changes in carbohydrate levels and mRNA levels of FT genes during cold treatment were also analysed. * One gene encodes sucrose-sucrose 1-fructosyltransferase (1-SST), and two gene encode fructan-fructan 6G-fructosyltransferase (6G-FFT). Protein sequences for the other genes (prfts 1, 2 and 6) were similar to sucrose-fructan 6-fructosyltransferase (6-SFT). The 1-SST and prft1 genes were colocalized with an invertase gene on the ryegrass linkage map. The mRNA levels of prft1 and prft2 increased gradually during cold treatment, while those of the 1-SST and 6G-FFT genes first increased, but then decreased before increasing again during a longer period of cold treatment. * Thus at least two different patterns of gene expression have developed during the evolution of functionally diverse FT genes, which are associated in a coordinated way with fructan synthesis in a cold environment.
The Journal of Agricultural Science | 2005
Mervyn O. Humphreys
Humphreys, M. O. (2005). Genetic improvement of forage crops - past, present and future. Journal of Agricultural Science, 143, (6), 441-448
Journal of Plant Physiology | 2001
Lesley B. Turner; Mervyn O. Humphreys; Andrew J. Cairns; Christopher J. Pollock
Turner, L. B., Humphreys, M. O., Cairns, A. J., Pollock, C. J. (2001). Comparison of growth and carbohydrate accumulation in seedlings of two varieties of Lolium perenne. Journal of Plant Physiology, 158, (7), 891-897. Sponsorship: BBSRC RASP Initiative
New Phytologist | 2008
Ian P. Armstead; Lesley B. Turner; Athole H. Marshall; Mervyn O. Humphreys; I. P. King; Daniel Thorogood
Mutational load and resource allocation factors and their effects on limiting seed set were investigated in ryegrass by comparative mapping genomics and quantitative trait loci (QTL) analysis in two perennial ryegrass (Lolium perenne) mapping families sharing common genetic markers. Quantitative trait loci for seed-set were identified on chromosome (LG) 7 in both families and on LG4 of the F2/WSC family. On LG7, seed-set and heading date QTLs colocalized in both families and cannot be unequivocally resolved. Comparative genomics suggests that the LG7 region is syntenous to a region of rice LG6 which contains both fertility (S5(n)) and heading date (Hd1, Hd3a) candidate genes. The LG4 region is syntenous to a region of rice LG3 which contains a fertility (S33) candidate gene. QTL maxima for seed-set and heading date on LG4 in the F2/WSC family are separated by c. 8 cm, indicating distinct genetic control. Low seed set is under the control of recessive genes at both LG4 and LG7 locations. The identification of QTLs associated with seed set, a major component of seed yield in perennial ryegrass, indicates that mutational load associated with these genomic regions can be mitigated through marker-assisted selection.
Euphytica | 1988
Mervyn O. Humphreys; C. F. Eagles
SummaryAssessments of freezing tolerance, estimated in terms of temperature at which 50% mortality occurs (=LT50), were obtained for 86 Lolium perenne accessions using a glycol bath test on naturally hardened field plants. Significant correlations were obtained between LT50 and both heading dates of accessions and minimum temperature of the coldest month near their sites of origin. The effects of temperature and reproductive development on the hardening/dehardening of plants and their growth after freezing are discussed.