Michael A. Cooper

Motor unit control strategies of endurance- versus resistance-trained individuals

Introduction: We examined motor unit (MU) control properties of resistance‐trained (RT) and endurance‐trained (ET) individuals. Methods: Five RT (age 25 ± 4 years) and 5 ET (age 19 ± 1 years) subjects participated. Surface electromyography (EMG) data were recorded from the vastus lateralis during isometric trapezoid muscle actions at 40% and 70% of maximal voluntary contraction. Decomposition and wavelet transform techniques were applied to the EMG signals to extract the firing events of single MUs and EMG intensity across the frequency spectrum. Results: There were significant differences between RT and ET for the mean MU firing rate and derecruitment threshold versus recruitment threshold relationships and EMG intensity at various wavelet bands during the linearly increasing, steady force, and linearly decreasing segments of the trapezoid contraction. Conclusions: MU behavior is altered as a function of training status and is likely the result of differences in the physical properties of the MU. Muscle Nerve 52: 832–843, 2015

Relationships between skinfold thickness and electromyographic and mechanomyographic amplitude recorded during voluntary and non-voluntary muscle actions

INTRODUCTION The purpose of this study was to examine possible correlations between skinfold thicknesses and the a terms from the log-transformed electromyographic (EMGRMS) and mechanomyographic amplitude (MMGRMS)-force relationships, EMG M-Waves, and MMG gross lateral movements (GLM). METHODS Forty healthy subjects performed a 6-s isometric ramp contraction from 5% to 85% of their maximal voluntary contraction with EMG and MMG sensors placed on the vastus lateralis (VL) and rectus femoris (RF). A single electrical stimulus was applied to the femoral nerve to record the EMG M-waves and MMG GLMs. Skinfold thickness was assessed at the site of each electrode. Pearsons product correlation coefficients were calculated comparing skinfold thicknesses with the a terms from the log-transformed EMGRMS-and MMGRMS-force relationships, EMG M-waves, and MMG GLMs. RESULTS There were no significant cor1relations (p>0.05) between the a terms and skinfold thicknesses for the RF and VL from the EMGRMS and MMGRMS-force relationships. However, there were significant correlations (p<0.05) between skinfold thicknesses and the EMG M-waves and MMG GLMs for the RF (r=-0.521, -0.376) and VL (r=-0.479, -0.484). DISCUSSION Relationships were only present between skinfold thickness and the amplitudes of the EMG and MMG signals during the non-voluntary muscle actions.

Instrument-assisted soft tissue mobilization: Effects on the properties of human plantar flexors

The effect of instrument-assisted soft tissue mobilization (ISTM) on passive properties and inflammation in human skeletal muscle has not been evaluated. Passive properties of muscle, inflammatory myokines and subjective reporting of functional ability were used to identify the effects of ISTM on the plantar flexors. 11 healthy men were measured for passive musculotendinous stiffness (MTS), passive range of motion (PROM), passive resistive torque (PASTQ) and maximum voluntary contraction peak torque (MVCPT) for plantar flexor muscles of the lower leg. Interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) were measured from muscle biopsies from the gastrocnemius, and subjective measurements of functional ability were taken using the perception of functional ability questionnaire (PFAQ). MTS, PROM, PRT and MVCPT were measured in the treatment leg (TL) and control leg (CL) before, immediately after, 24 h, 48 h and 72 h following IASTM. Biopsies for IL-6 and TNF-α and PFAQ responses were collected before as well as 24 h, 48 h and 72 h after IASTM. There were no significant differences in MTS, PROM, PASTQ, MVCPT, IL-6 and TNF-α between the TL or CL. A significant decrease in the perception of function and a significant increase in pain for the TL were found following IASTM.

Emerging Relationships Between Exercise, Sensory Nerves, and Neuropathic Pain

The utilization of physical activity as a therapeutic tool is rapidly growing in the medical community and the role exercise may offer in the alleviation of painful disease states is an emerging research area. The development of neuropathic pain is a complex mechanism, which clinicians and researchers are continually working to better understand. The limited therapies available for alleviation of these pain states are still focused on pain abatement and as opposed to treating underlying mechanisms. The continued research into exercise and pain may address these underlying mechanisms, but the mechanisms which exercise acts through are still poorly understood. The objective of this review is to provide an overview of how the peripheral nervous system responds to exercise, the relationship of inflammation and exercise, and experimental and clinical use of exercise to treat pain. Although pain is associated with many conditions, this review highlights pain associated with diabetes as well as experimental studies on nerve damages-associated pain. Because of the global effects of exercise across multiple organ systems, exercise intervention can address multiple problems across the entire nervous system through a single intervention. This is a double-edged sword however, as the global interactions of exercise also require in depth investigations to include and identify the many changes that can occur after physical activity. A continued investment into research is necessary to advance the adoption of physical activity as a beneficial remedy for neuropathic pain. The following highlights our current understanding of how exercise alters pain, the varied pain models used to explore exercise intervention, and the molecular pathways leading to the physiological and pathological changes following exercise intervention.

Modulation of diet-induced mechanical allodynia by metabolic parameters and inflammation

Dietary‐associated diseases have increased tremendously in our current population, yet key molecular changes associated with high‐fat diets that cause clinical pre‐diabetes, obesity, hyperglycemia, and peripheral neuropathy remain unclear. This study examines molecular and metabolic aspects altered by voluntary exercise and a high‐fat diet in the mouse dorsal root ganglion. Mice were examined for changes in mRNA and proteins encoding anti‐inflammatory mediators, metabolic‐associated molecules, and pain‐associated ion channels. Proteins involved in the synaptosomal complex and pain‐associated TRP ion channels decrease in the dorsal root ganglion of high‐fat exercise animals relative to their sedentary controls. Exercise reversed high‐fat diet induced mechanical allodynia without affecting weight gain, elevated blood glucose, and utilization of fat as a fuel source. Independent of weight or fat mass changes, high‐fat exercised mice display reduced inflammation‐associated mRNAs. The benefits of exercise on abnormal peripheral nerve function appear to occur independent of systemic metabolic changes, suggesting that the utilization of fats and inflammation in the peripheral nervous system may be key for diet‐induced peripheral nerve dysfunction and the response to exercise.

Muscle-related differences in mechanomyography–force relationships are model-dependent.

Introduction: In this study we examined the mechanomyographic amplitude (MMGRMS)–force relationships with log‐transform and polynomial regression models for the vastus lateralis (VL), rectus femoris (RF), and first dorsal interosseous (FDI) muscles. Methods: Twelve healthy (age 23 ± 3 years) men performed isometric ramp contractions of the leg extensors and index finger from 10% to 80% of their maximal voluntary contraction (MVC) with MMG sensors positioned on the VL, RF, and FDI. Log‐transform and polynomial regression models were fitted to the relationships. Results: There were differences for the a terms (intercepts) and b terms (slopes) from the log‐transform model between the FDI, VL, and RF; however, there were no consistent differences identified with the polynomial regression models. Conclusions: The log‐transform model quantified differences in the patterns of responses between the FDI and the leg extensors, but polynomial regression could not distinguish such differences. Muscle Nerve 49: 202–208, 2014

Examination of motor unit control properties of the vastus lateralis in an individual that had acute paralytic poliomyelitis.

Summary: The purpose of the study was to examine motor unit (MU) recruitment and derecruitment thresholds and firing rates of the vastus lateralis between 2 healthy (HE) individuals (women, ages = 19 and 23 years) and 1 individual (man, age = 22 years) who acquired acute poliomyelitis (PO). Each participant performed submaximal isometric trapezoid muscle actions of the leg extensors from 20% to 90% maximal voluntary contraction in 10% increments with a sensor placed on the vastus lateralis to record electromyography. Electromyographic signals were decomposed into the firing events of single MUs. Linear regressions were performed on the firing rates at recruitment and peak firing rates versus the recruitment thresholds and the derecruitment versus recruitment thresholds. In addition, data were pooled together from all contractions to examine differences between PO and HE with independent samples t-tests calculated for firing rates at recruitment, peak firing rates, recruitment thresholds, derecruitment thresholds, and duration of MU activity. The results demonstrated systematic differences in MU control strategies between the PO and HE. There were differences in the recruitment thresholds (P < 0.001; HE = 30.5% ± 22.2% maximal voluntary contraction; PO = 14.5% ± 5.0% maximal voluntary contraction), firing rates at recruitment (P < 0.001; HE = 7.4 ± 2.5 pulses per second; PO = 6.2 ± 1.7 pulses per second) and peak firing rates across the force spectrum (P = 0.001; HE = 22.2 ± 5.8 pulses per second; PO = 20.3 ± 2.3 pulses per second), altered derecruitment versus recruitment relationships (HE slope = 0.82 derec/rec, PO slope = 1.78 derec/rec), and duration of MU activity (P < 0.001) between the PO (18.6 ± 2.4 seconds) and HE (15.3 ± 3.0 seconds). Future research should examine the possible differences in MU behavior between PO and HE as a result of fatigue to further elucidate disease-related changes in MU properties.

Endocrine responses and acute mTOR pathway phosphorylation to resistance exercise with leucine and whey

Leucine ingestion reportedly activates the mTOR pathway in skeletal muscle, contributing to a hypertrophy response. The purpose of the study was to compare the post-resistance exercise effects of leucine and whey protein supplementation on endocrine responses and muscle mTOR pathway phosphorylation. On visit 1, subjects (X±SD; n=20; age=27.8±2.8yrs) provided baseline blood samples for analysis of cortisol, glucose and insulin; a muscle biopsy of the vastus lateralis muscle to assess mTOR signaling pathway phosphorylation; and were tested for maximum strength on the leg press and leg extension exercises. For visits 2 and 3, subjects were randomized in a double-blind crossover design to ingest either leucine and whey protein (10g+10g; supplement) or a non-caloric placebo. During these visits, 5 sets of 10 repetitions were performed on both exercises, immediately followed by ingestion of the supplement or placebo. Blood was sampled 30 min post-, and a muscle biopsy 45 min post-exercise. Western blots quantified total and phosphorylated proteins. Insulin increased (α<.05) with supplementation with no change in glucose compared to placebo. Relative phosphorylation of AKT and rpS6 were greater with leucine and whey supplementation compared to placebo. Supplementation of leucine and whey protein immediately after heavy resistance exercise increases anabolic signaling in human skeletal muscle.

The reliability of the interpolated twitch technique during submaximal and maximal isometric muscle actions.

Abstract Cooper, MA, Herda, TJ, Walter-Herda, AA, Costa, PB, Ryan, ED, and Cramer, JT. The reliability of the interpolated twitch technique during submaximal and maximal isometric muscle actions. J Strength Cond Res 27(10): 2909–2913, 2013—The purpose of this study was to examine the test-retest reliability of the percent voluntary activation (%VA) vs. force relationships. Fourteen healthy men (mean ± SD age = 21 ± 2.6 years) and 8 women (age = 21 ± 1.8 years) completed 4 maximal voluntary contractions (MVCs) and 9 randomly ordered submaximal isometric plantar flexions from 10 to 100% of the MVC. Transcutaneous electrical stimuli were delivered to the tibial nerve using a high-voltage constant-current stimulator (DS7AH; Digitimer, Herthfordshire, United Kingdom). The %VA was calculated for each maximal and submaximal MVC. Paired-samples t-tests were used to quantify systematic variability, whereas the intraclass correlation coefficients (ICCs), standard error of the mean (%SEM), and minimum differences (%MD; expressed as a percentage of the means) were used for test-retest reliability. Systematic variability was not present at any of the contraction intensities (p > 0.05). The ICCs ranged from 0.52 to 0.84, whereas the %SEM ranged from 6.75 to 38.45%, and the %MD ranged from 18.71 to 106.58%. The ICCs were ≥0.74 at contraction intensities ranging from 40 to 100% MVC (6.75–16.78% SEM), whereas the ICCs were ⩽0.65 (20.95–38.45% SEM) for the contraction intensities ⩽30% MVC. Although not statistically tested, the ICCs tended to be higher, whereas the %SEMs lower for contractions ≥40% MVC. Future research using %VA during submaximal contraction intensities to predict a true maximal force may want to exclude contraction intensities <40% MVC. In addition, caution is warranted when interpreting the changes in the %VA during MVCs after an experimental intervention.

Electromyographic, but not mechanomyographic amplitude-force relationships, distinguished differences in voluntary activation capabilities between individuals

The purpose of the present study was to examine the influence of activation capabilities on the electromyography (EMGRMS) and mechanomyography amplitude (MMGRMS)-force relationships of the vastus lateralis (VL) and rectus femoris (RF). Thirteen men (mean±SD; age=22±3 year) performed nine submaximal contractions (10-90% maximal voluntary contraction [MVC]) with the interpolated twitch technique performed during a separate contraction at 90% MVC to calculate percent voluntary activation (%VA). Nine participants with >90% VA were categorized into the high-activated group with the remaining categorized into the moderate-activated group. Slopes (b terms) were calculated from the log-transformed EMGRMS and MMGRMS-force relationships. The b terms (collapsed across the VL and RF) for the EMGRMS-force relationships were greater for the high- (1.29±0.31) than the moderate-activated (1.10±0.20) group. In contrast, there were no differences in the b terms for the MMGRMS-force relationships between the high- and moderate-activated groups. For the EMGRMS and MMGRMS-force relationships, the b terms were greater for the RF (1.38±0.30, 0.81±0.20) than the VL (1.08±0.19, 0.60±0.13) collapsed across groups. The b terms from the EMGRMS-force relationships, but not the MMGRMS-force relationships, reflected differences in %VA.