Michael D. Edge | Researchain

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Tetrahedron Letters | 1989

A chemical method of labelling oligodeoxyribonucleotides with biotin: A single step procedure using a solid phase methodology

Anne M. Alves; David Holland; Michael D. Edge

Abstract Protected D(+)-biotin methylesters 3 were reduced to the corresponding alcohols 4 and phosphitylated with chloromethoxymorpholinophosphine. The resulting phosphoramidites 5 were used on a commercial DNA synthesiser to biotinylate synthetic DNA.

Analytical Biochemistry | 1983

Ion-exchange high-performance liquid chromatography of oligodeoxyribonucleotides using formamide.

Clive R. Newton; April R. Greene; Gillian R. Heathcliffe; Thomas C. Atkinson; David Holland; Alexander F. Markham; Michael D. Edge

The superiority of buffer systems containing formamide for the ion-exchange high-performance liquid chromatographic separation of oligodeoxyribonucleotide mixtures generated in solid-phase syntheses is illustrated. The resolutions achieved are compared to those achieved with the same mixtures in other eluting solvents. The use of formamide systems is recommended for oligodeoxyribonucleotide purification in general and is particularly valuable where the oligonucleotide of interest is highly self-complementary and/or rich in deoxyguanosine residues.

Nature | 1981

Total synthesis of a human leukocyte interferon gene.

Michael D. Edge; April R. Greene; Gillian R. Heathcliffe; Peter Anthony Meacock; Wolfgang Schuch; Denis B. Scanlon; Thomas C. Atkinson; Clive R. Newton; Alexander Fred Markham

Nucleic Acids Research | 1993

The production of PCR products with 5′ single-stranded tails using primers that incorporate novel phosphoramidite intermediates*

Clive R. Newton; David Holland; L.E. Heptinstall; I. Hodgson; Michael D. Edge; Alexander F. Markham; M.J. McLean

Nucleic Acids Research | 1980

Rapid synthesis of oligodeoxyribonucleotides IV. Improved solid phase synthesis of oligodeoxy-ribonucleotides through phosphotriester intermediates

M.J. Gait; M. Singh; R.C. Sheppard; Michael D. Edge; A.R. Greene; G.R. Heathcliffe; T.C. Atkinson; Clive R. Newton; Alexander F. Markham

Nucleic Acids Research | 1983

Chemical synthesis of a human interferon-α2, gene and its expression in Escherichia coli

Michael D. Edge; A.R. Greene; G.R. Heathcliffe; V. E. Moore; N.J. Faulkner; R. Camble; N.N. Petter; P. Trueman; Wolfgang Schuch; J. Hennam; T.C. Atkinson; Clive R. Newton; A.F. Markham

Nucleic Acids Research | 1980