Michael Leuck

Novel Method for the Covalent Immobilization of Oligonucleotides via Diels-Alder Bioconjugation

Abstract The synthesis of cyclohexadiene and maleimide derivatives and their use for the functionalization of oligonucleotides and the coating of glass surfaces is reported. A method for the covalent attachment of diene or maleimide modified oligonucleotides to the coated glass surfaces via aqueous Diels-Alder reactions is presented.

A NOVEL AMINO-ON CPG-SUPPORT FOR THE SYNTHESIS OF 3′-AMINOALKYLATED OLIGONUCLEOTIDES

The synthesis of a novel amino-ON CPG support and its application in the synthesis of 3′-aminoalkylated oligonucleotides is reported. The release of oligonucleotides with free 3′-amino groups is accomplished by treatment with concentrated ammonia for 2 h at 55°C.

Synthesis of Oligonucleotide Conjugates via Aqueous Diels‐Alder Cycloaddition

The conjugation of maleimide reporter groups to 5′‐diene‐modified oligonucleotides via aqueous Diels‐Alder cycloaddition is described. Detailed basic protocols are provided for the preparation of a diene‐amidite (5′‐diene modifier), for the attachment of a diene group to the 5′‐terminus of oligonucleotides, and for the conjugation of maleimide reporter groups to diene‐modified oligonucleotides.