Michael P. Murphy

Exogenous endothelial cells as accelerators of hematopoietic reconstitution

Despite the successes of recombinant hematopoietic-stimulatory factors at accelerating bone marrow reconstitution and shortening the neutropenic period post-transplantation, significant challenges remain such as cost, inability to reconstitute thrombocytic lineages, and lack of efficacy in conditions such as aplastic anemia. A possible means of accelerating hematopoietic reconstitution would be administration of cells capable of secreting hematopoietic growth factors. Advantages of this approach would include: a) ability to regulate secretion of cytokines based on biological need; b) long term, localized production of growth factors, alleviating need for systemic administration of factors that possess unintended adverse effects; and c) potential to actively repair the hematopoietic stem cell niche. Here we overview the field of hematopoietic growth factors, discuss previous experiences with mesenchymal stem cells (MSC) in accelerating hematopoiesis, and conclude by putting forth the rationale of utilizing exogenous endothelial cells as a novel cellular therapy for acceleration of hematopoietic recovery.

Animal Studies of Cord Blood and Regeneration

Cord blood has been used successfully as an alternative stem cell source to marrow, particularly in pediatric patients with hematopoietic malignancies, bone marrow failure, or inborn errors of metabolism, and currently expanding to adults. Cord blood was known since the 1930s to be useful as a substitute for peripheral blood in transfusions.