Michael Schagerl

The Danube restoration project: functional aspects and planktonic productivity in the floodplain system

In Austria, the ‘Danube Restoration Project’ (DRP) was implemented to re-establish the connectivity between the Danube and its floodplain along a free flowing section downstream of Vienna. Before the restoration, the status quo of the ‘Regelsbrunn’ floodplain segment was investigated at different levels of abiotic, biotic and functional properties according to the spatio-temporal effects of hydrological connectivity. The present paper deals with temporal effects on hydrochemistry and on phytoand bacterioplankton in the side channel (parapotamon). A hydrological situation of the present state of short-termed and intensive flood pulses followed by a period of isolation was compared with a period of higher connectivity reflecting the situation after the restoration. Rising water levels in the river, establishing local surface inflows, resulted in periodic nutrient pulses, high particle load and eutrophication in the floodplain. With disconnection after a spate, the nitrate and soluble reactive phosphate (SRP) concentration decreased significantly during 1 month. Phytoplankton correlated negatively with SRP during the first week after a flood pulse, reflecting the dominance of phytoplankton in SRP uptake. Planktonic primary production was stimulated by the nutrient import at higher connectivity and could be controlled by a decrease in retention time, related to the aims of the DRP. The two investigated periods indicated that increasing retention time in the side channel resulted in a shift from primary production towards prevailing bacterial secondary production. Copyright

Phytoplankton community relationship to environmental variables in three Kenyan Rift Valley saline-alkaline lakes

Temporal changes in total alkalinity, ionic composition and nutrient concentrations were studied in the saline, alkaline endorheic Kenyan Rift Valley Lakes Bogoria, Nakuru and Elmentaita to understand the association of these variables with phytoplankton community structure. In total, 24 taxa were found, with L. Bogoria having the fewest species. Although the cyanobacterium Arthrospira fusiformis dominated the phytoplankton biomass, especially in L. Bogoria, other groups came into play especially during high water levels in L. Nakuru and L. Elmentaita. Cluster analysis based on species biomass resulted in four groups, characterised by 13 indicator taxa. Most of the variation in these groups appeared to be associated with hydrological stability and perhaps biological factors rather than water chemistry, which only explained 44% of the variance in taxa composition on the first four axes derived from redundancy analysis. Species numbers decreased with elevated conductivity and water temperature. Synechocystis sp. occurrence coincided with phosphorus, water temperature and conductivity increase, whereas the distributions of Arthrospira fusiformis and Arthrospira platensis were mainly influenced by both light attenuation and elevated nitrate concentrations. Increases in silica and ammonium and declines in conductivity, total phosphorus and water temperature enhanced diatom abundances. Not only do the results of the present study indicate the unexpectedly high variability of phytoplankton community composition and water chemistry in these three alkaline tropical lakes, but also the data assist our understanding of the factors influencing flamingo populations on these lakes, which are significant conservation reserves and tourist attractions.

Temporal trends of ion contents and nutrients in three Kenyan Rift Valley saline-alkaline lakes and their influence on phytoplankton biomass

This study, carried out between November 2003 and February 2005, aimed to investigate the temporal trends of conductivity, ions, nutrient concentrations and phytoplankton biomass expressed as chlorophyll a in the Kenyan Rift Valley saline-alkaline lakes namely Nakuru, Bogoria and Elmentaita. The influence of environmental variables on phytoplankton biomass has always been of much interest in understanding phytoplankton dynamics. Being shallow and endorheic, these lakes’ chemical, physical and biological properties were found to be strongly influenced by the hydrologic cycle within their catchment area. The lakes are characterised by high nutrient concentrations but with low Ntot: Ptot ratios. Significant differences between surface and near-bottom samples for water temperature, chlorophyll a and some nutrients were found in these lakes. A stepwise Discriminant Analysis with lakes as defined groups resulted in a significant model with SRP, nitrate-N, conductivity and light supply being of major importance. A significant correlation between specific conductivity and total alkalinity (Kendalls τ = 0.85, n = 132) was calculated. While L. Bogoria showed the least temporal variation in conductivity-(65-73 mS cm−1), larger variations were observed in L. Elmentaita (21-77 mS cm−1). Na+ and K+ form the main cations with Cl−, HCO3 − and CO3 2 being the major anions in all the three lakes. Flouride was detected in high quantities (mean values L. Bogoria 72 meq 1−1, L. Nakuru 17 meq 1−1 and L. Elmentaita 71 meq 1−1). A PCA followed by multiple regression analysis with chlorophyll a as dependent variable showed that nitrate-N, conductivity, phosphorus and light supply were the key variables influencing algal biomass in these lakes.

Allelopathy among Cyanoprokaryota and Other Algae Originating from Lake Neusiedlersee (Austria)

During the late eighties, the eutrophic shallow lake Neusiedlersee located at the austrian - hungarian border showed a massive development of cyanobacteria, which coincided with a strong decline of chlorococcales. Besides a change in nutrient conditions, allelopathy may have contributed to the dominance of cyanobacteria, too. To get a first insight into potential allelopathic effects, 13 cyanobacteria strains were tested originating for the most part from lake Neusiedlersee. Plate diffusion assays showed allelopathic activity of 6 strains indicating chemical interactions between cyanobacteria and other algae.

Integrated management to restore macrophyte domination

Recentchanges which have been observed at Alte Donau, a shallow urban lake within the city of Vienna, have been interpreted as a shift to a new stable state. The former macrophyte-dominated state changed to a turbid state dominated by high biomass of filamentous cyanobacteria, associated with a significant reduction in Secchi-depth. Phytoplankton was dominated by the filamentous cyanobacterial species Cylindrospermopsis raciborskii (Wolosz.), Seenayya et Subba Raju and Limnothrix redekei (Van Goor) Meffert. Integrated restoration plans included internal and external measures. Improvements in the catchment aim to minimize the input of nutrients from contaminated groundwater and from storm water and to reduce large numbers of water fowl. Internal restoration measures included water exchange, chemical flocculation and nitrate oxidation of the sediments. Additionally, macrophyte re-colonisation was enhanced through planting. A pelagic predator (Aspius aspius L.) was stocked to reduce bleak (Alburnus alburnus L.), the dominant cyprinid planktivore. Results from the period after water exchange and chemical treatment, showed significant reduction of nutrient and chlorophyll a concentrations. A shift in the phytoplankton species from cyanobacteria towards diatoms and greens was observed. Secchi depth greatly increased. Macrophyte growth became apparent both through re-colonisation, as well as from the planting.

A detailed time series assessment of the diet of Lesser Flamingos: further explanation for their itinerant behaviour

We hypothesised that changes in nutritional composition and quality of the pelagic phytoplankton community influence the occurrence of Lesser Flamingo populations in two Kenyan saline–alkaline lakes, Nakuru and Bogoria. This was achieved by carrying out a detailed time series assessment of the phytoplankton community composition and nutritional components (carbohydrates, crude protein and lipids) from July 2008 to October 2009 on a weekly basis for each lake. Lesser Flamingos were estimated visually from the lake shore of the sampling sites. In Lake Nakuru, Lesser Flamingos had a significant positive relationship with lipids and Arthrospira biomass but a negative relationship with small cyanoprokaryotes. For Lake Bogoria, no significant differences were observed as the Arthrospira was available throughout the sampling period though the flamingo numbers still fluctuated. We concluded that the nutritional composition and quality of the phytoplankton community influence the temporal and spatial abundance of Lesser Flamingos although other factors such as the prevailing environmental conditions may take precedence.

The virus's tooth: cyanophages affect an African flamingo population in a bottom-up cascade

Trophic cascade effects occur when a food web is disrupted by loss or significant reduction of one or more of its members. In East African Rift Valley lakes, the Lesser Flamingo is on top of a short food chain. At irregular intervals, the dominance of their most important food source, the cyanobacterium Arthrospira fusiformis, is interrupted. Bacteriophages are known as potentially controlling photoautotrophic bacterioplankton. In Lake Nakuru (Kenya), we found the highest abundance of suspended viruses ever recorded in a natural aquatic system. We document that cyanophage infection and the related breakdown of A. fusiformis biomass led to a dramatic reduction in flamingo abundance. This documents that virus infection at the very base of a food chain can affect, in a bottom-up cascade, the distribution of end consumers. We anticipate this as an important example for virus-mediated cascading effects, potentially occurring also in various other aquatic food webs.

Allelopathic activity of Chara aspera

Allelopathic activity of Chara aspera was determined in agar diffusion assays using planktonic cyanobacteria as target organisms. Growth inhibition of cyanobacterial strains was observed in bioassays inoculated with living Chara aspera shoots as well as with 60% aqueous methanol extracts of Chara aspera. For further analysis, the methanol extract was fractionated into three parts: a lipophilic methanol – a butylmethylether-extract and a hydrophilic methanol extract. The bioassays indicated that major allelopathic activity was retained in the hydrophilic methanol – and the lipophilic butylmethylether-extract. Separation of the extracts by means of high performance liquid chromatography followed by fractionation of the eluant resulted in supplementary nine fractions, three from each part, respectively. Three fractions exhibited a strong growth inhibition of the target organism Anabaena cylindrica Lemmermann. The second and the third fraction of the lipophilic butylmethylether extract indicate the presence of novel allelopathic active compounds with lipophilic characteristics. The results lead to the suggestion that more than two chemical compounds in Chara aspera are responsible for the growth inhibition of cyanobacteria.

Microzooplankton feeding behaviour: grazing on the microbial and the classical food web of African soda lakes

We investigated the feeding behaviour of the dominant microzooplankton of saline lakes in the East African Rift Valley. A set of grazing experiments revealed high ingestion rates of the two euryhaline rotifers Brachionus dimidiatus and Brachionus plicatilis and of the large-sized omnivorous ciliates Frontonia sp. and Condylostoma magnum reflecting the unique nature of tropical saline systems. The size spectrum of ingested particles was broad and even included filamentous cyanobacteria such as the commonly dominating Arthrospira fusiformis. Feeding selectivity on cyanobacteria, however, was rather low showing higher values for cryptomonads and small ciliates. Bacterial biomass was favoured by the presence of grazers, as small bacterivorous predators were reduced at an average of 13.9%, showing the cascading effect of large zooplankton on the food web structure. Overall, based on this first-time study of the microzooplankton feeding behaviour in East African soda lakes, a strong structuring effect of rotifers and large ciliates on microbial plankton communities is assumed, especially in times of high consumer biomass.

Pigment composition of freshwater charophyceae

Abstract Twelve species of Charophyceae were collected at different locations in the eastern part of Austria and photosynthetic pigments analyzed by means of rP-HPLC (reversed phase-high performance liquid chromatography). Besides pigments typical of higher plants, also γ-carotene was detected in antheridia and for the first time in sterile specimens of Chara tomentosa. Probably there exists two fractions of s-carotene, one closely associated with γ-carotene in separate compartments, the other independent of γ-carotene and located in the photosystems. Chlorophyll a on the basis of ash-free dry mass was comparable to that of planktonic algae and submerged angiosperms (about 0.5%), but pigment content varied in a wide range, suggesting adaptations to ecological conditions such as light or temperature. Our results indicate that fresh and dry mass are not suitable as a reference quantity in stoneworts due to high calcium carbonate precipitation.