Michael Seul

A flexible array format for large‐scale, rapid blood group DNA typing

BACKGROUND: Typing for blood group antigens is currently performed by hemagglutination. The necessary reagents are becoming costly and limited in availability, and the methods are labor‐intensive. The purpose of this study was to determine the feasibility of the use of large‐scale DNA analysis in a microarray as a substitute for blood group typing.

Determination of 24 minor red blood cell antigens for more than 2000 blood donors by high‐throughput DNA analysis

BACKGROUND: A “BeadChip” array permits reliable simultaneous DNA typing of single‐nucleotide polymorphisms for minor blood groups. A high‐throughput DNA analysis was studied as a routine method of phenotype prediction and software was developed to interpret and analyze the large volume of data points.

Toward extended phenotype matching: a new operational paradigm for the transfusion service

BACKGROUND: Conventional pretransfusion testing uses hemagglutination to ensure donor‐recipient compatibility for ABO/D status and recipient alloantibodies. While screening large numbers of donor units for multiple antigens by hemagglutination is impractical, novel methods of DNA analysis permit the rapid determination of an extended human erythrocyte antigen (xHEA) phenotype. A prospective observational study was conducted at four hospital transfusion services to test an alternative paradigm of identifying xHEA‐typed units for patients in three cohorts by utilizing DNA analysis and a novel inventory management model.

TRANSFUSION PRACTICE: Toward extended phenotype matching: a new operational paradigm for the transfusion service

BACKGROUND: Conventional pretransfusion testing uses hemagglutination to ensure donor‐recipient compatibility for ABO/D status and recipient alloantibodies. While screening large numbers of donor units for multiple antigens by hemagglutination is impractical, novel methods of DNA analysis permit the rapid determination of an extended human erythrocyte antigen (xHEA) phenotype. A prospective observational study was conducted at four hospital transfusion services to test an alternative paradigm of identifying xHEA‐typed units for patients in three cohorts by utilizing DNA analysis and a novel inventory management model.

The BeadChip System: A Flexible Array Format for Complex Nucleic Acid and Protein Analysis

This chapter provides an overview of key elements of the bead array technology and related manufacturing steps as well as key components in the deployment of the BeadChip™ system. The BeadChip™ molecular immunohematology has a spectrum of applications which can utilize multianalyte (multiplex) nucleic acid and protein analysis by Random Encoded Array Detection.

TRANSFUSION PRACTICE: Toward extended phenotype matching: a new operational paradigm for the transfusion service: TOWARD EXTENDED PHENOTYPE MATCHING

BACKGROUND: Conventional pretransfusion testing uses hemagglutination to ensure donor‐recipient compatibility for ABO/D status and recipient alloantibodies. While screening large numbers of donor units for multiple antigens by hemagglutination is impractical, novel methods of DNA analysis permit the rapid determination of an extended human erythrocyte antigen (xHEA) phenotype. A prospective observational study was conducted at four hospital transfusion services to test an alternative paradigm of identifying xHEA‐typed units for patients in three cohorts by utilizing DNA analysis and a novel inventory management model.