Network


Latest external collaboration on country level. Dive into details by clicking on the dots.

Hotspot


Dive into the research topics where Michele L. Fields is active.

Publication


Featured researches published by Michele L. Fields.


Immunity | 2002

The Impact of T Helper and T Regulatory Cells on the Regulation of Anti-Double-Stranded DNA B Cells

Su-jean Seo; Michele L. Fields; Jodi L. Buckler; Amy J. Reed; Laura Mandik-Nayak; Simone A. Nish; Randolph J. Noelle; Laurence A. Turka; Fred D. Finkelman; Andrew J. Caton; Jan Erikson

Autoreactive B cells that appear to be inactivated can be found in healthy individuals. In this study, we examined the potential of these anergic cells to become activated. We show that anergy of anti-double-stranded DNA (dsDNA) B cells in BALB/c mice is readily reversed, requiring only the provision of T cell help. We further show that spontaneous loss of anergy among anti-dsDNA B cells in autoimmune lpr/lpr mice occurs in two phases: an abortive initial response to T help followed by full loss of tolerance. Strikingly, the abortive response can be reproduced in nonautoimmune mice when CD4+CD25+ T regulatory cells are administered in conjunction with CD4+ T helper cells, suggesting that loss of B cell tolerance may require both the production of T cell help and the overcoming of T suppression.


Journal of Immunology | 2005

CD4+CD25+ Regulatory T Cells Inhibit the Maturation but Not the Initiation of an Autoantibody Response

Michele L. Fields; Brian D. Hondowicz; Michele H. Metzgar; Simone A. Nish; Gina N. Wharton; Cristina Cozzo Picca; Andrew J. Caton; Jan Erikson

To investigate the mechanism by which T regulatory (Treg) cells may control the early onset of autoimmunity, we have used an adoptive transfer model to track Treg, Th, and anti-chromatin B cell interactions in vivo. We show that anti-chromatin B cells secrete Abs by day 8 in vivo upon provision of undeviated, Th1- or Th2-type CD4+ T cell help, but this secretion is blocked by the coinjection of CD4+CD25+ Treg cells. Although Treg cells do not interfere with the initial follicular entry or activation of Th or B cells at day 3, ICOS levels on Th cells are decreased. Furthermore, Treg cells must be administered during the initial phases of the Ab response to exert full suppression of autoantibody production. These studies indicate that CD25+ Treg cells act to inhibit the maturation, rather than the initiation, of autoantibody responses.


Journal of Immunology | 2002

Chronic Graft-Versus-Host in Ig Knockin Transgenic Mice Abrogates B Cell Tolerance in Anti-Double-Stranded DNA B Cells

Debora R. Sekiguchi; Sandra M. Jainandunsing; Michele L. Fields; Michael A. Maldonado; Michael P. Madaio; Jan Erikson; Martin Weigert; Robert A. Eisenberg

Anti-dsDNA Abs are specific diagnostic markers of systemic lupus erythematosus, and are also implicated in kidney pathology. Anti-dsDNA B cells have been shown to be tolerized in nonautoimmune mice. The immunodysregulation that causes these cells to break tolerance is presumably part of the fundamental defects in systemic lupus erythematosus. To explore these mechanisms, we used the chronic graft-versus-host model mediated by MHC class II differences. Induction of chronic graft-vs-host in anti-DNA H chain knockin (3H9.KI) transgenic mice on a nonautoimmune background resulted in specific activation of anti-dsDNA B cells, as evidenced by high titers of soluble Ab in sera and a high frequency (70%) of anti-dsDNA B cell clones recovered as hybridomas. In addition, the λ+-anti-dsDNA B cells developed increased expression of cell surface activation markers, and concentrated in the T cell area of the follicle with an Ab-forming cell-compatible phenotype. Genetic analysis of the hybridoma clones showed strong evidence of secondary rearrangements of the L chain associated with anti-dsDNA reactivity. Thus, our study indicates that alloreactive T cell help can break tolerance in a complex manner, involving several events.


Journal of Immunology | 2001

Fas/Fas Ligand Deficiency Results in Altered Localization of Anti-Double-Stranded DNA B Cells and Dendritic Cells

Michele L. Fields; Caroline L. Sokol; Ashlyn Eaton-Bassiri; Su-jean Seo; Michael P. Madaio; Jan Erikson

Autoantibodies directed against dsDNA are found in patients with systemic lupus erythematosus as well as in mice functionally deficient in either Fas or Fas ligand (FasL) (lpr/lpr or gld/gld mice). Previously, an IgH chain transgene has been used to track anti-dsDNA B cells in both nonautoimmune BALB/c mice, in which autoreactive B cells are held in check, and MRL-lpr/lpr mice, in which autoantibodies are produced. In this study, we have isolated the Fas/FasL mutations away from the autoimmune-prone MRL background, and we show that anti-dsDNA B cells in Fas/FasL-deficient BALB/c mice are no longer follicularly excluded, and they produce autoantibodies. Strikingly, this is accompanied by alterations in the frequency and localization of dendritic cells as well as a global increase in CD4 T cell activation. Notably, as opposed to MRL-lpr/lpr mice, BALB-lpr/lpr mice show no appreciable kidney pathology. Thus, while some aspects of autoimmune pathology (e.g., nephritis) rely on the interaction of the MRL background with the lpr mutation, mutations in Fas/FasL alone are sufficient to alter the fate of anti-dsDNA B cells, dendritic cells, and T cells.


Journal of Immunology | 2006

Exogenous and Endogenous TLR Ligands Activate Anti-Chromatin and Polyreactive B Cells

Michele L. Fields; Michele H. Metzgar; Brian D. Hondowicz; Sun-Ah Kang; Shawn T. Alexander; Kristin Hazard; Alice C. Hsu; Yangzhu Du; Eline T. Luning Prak; Marc Monestier; Jan Erikson

Autoreactive B cells may become activated in a T-independent manner via synergistic engagement of the BCR and TLRs. Using the VH3H9 Ig H chain transgene to track anti-chromatin B cells, we demonstrate that VH3H9/Vλ1 anti-chromatin B cells proliferate in response to stimulatory oligodeoxynucleotides containing CpG motifs, suggesting that these autoreactive B cells are responsive to TLR9 signaling. Strikingly, some VH3H9 B cells, but not the well-characterized VH3H9/Vλ1 B cells, proliferate spontaneously in culture medium. This proliferation is blocked by inhibitory CpG oligodeoxynucleotides, implicating the TLR9 (or possibly TLR7) pathway. Most hybridomas generated from the proliferating cells are polyreactive, and one exhibits binding to nuclear Ags but not to the other Ags tested. Thus, B cells carrying autoreactive and/or polyreactive specificities may be susceptible to T cell-independent activation via dual engagement of the BCR and TLRs.


Current Opinion in Immunology | 2008

Role of B cells in Systemic Lupus Erythematosus and Rheumatoid Arthritis

Laura Mandik-Nayak; Natalie Ridge; Michele L. Fields; Audrey Y. Park; Jan Erikson

B cell tolerance to many self-proteins is actively maintained by either purging self-reactive B receptors through clonal deletion and receptor editing, or by functional silencing known as anergy. However, these processes are clearly incomplete as B cell driven autoimmune diseases still occur. The significance of B cells in two such diseases, rheumatoid arthritis and systemic lupus erythematosus, is highlighted by the ameliorative effects of B cell depletion. It remains to be determined, however, whether the key role of the B cell in autoimmune disease is autoantibody production or another antibody-independent function.


Journal of Immunology | 2005

The Influence of Effector T Cells and Fas Ligand on Lupus-Associated B Cells

Michele L. Fields; Simone A. Nish; Brian D. Hondowicz; Michele H. Metzgar; Gina N. Wharton; Andrew J. Caton; Jan Erikson

Circulating autoantibodies against dsDNA and chromatin are a characteristic of systemic lupus erythematosus in humans and many mouse models of this disease. B cells expressing these autoantibodies are normally regulated in nonautoimmune-prone mice but are induced to secrete Abs following T cell help. Likewise, anti-chromatin autoantibody production is T cell-dependent in Fas/Fas ligand (FasL)-deficient (lpr/lpr or gld/gld) mice. In this study, we demonstrate that Th2 cells promote anti-chromatin B cell survival and autoantibody production in vivo. FasL influences the ability of Th2 cells to help B cells, as Th2-gld/gld cells support higher titers of anti-chromatin Abs than their FasL-sufficient counterparts and promote anti-chromatin B cell participation in germinal centers. Th1 cells induce anti-chromatin B cell germinal centers regardless of FasL status; however, their ability to stimulate anti-chromatin Ab production positively correlates with their level of IFN-γ production. This distinction is lost if FasL-deficient T cells are used: Th1-gld/gld cells promote significant titers of anti-chromatin Abs regardless of IFN-γ production levels. Thus, FasL from effector T cells plays an important role in determining the fate of anti-chromatin B cells.


Immunological Reviews | 2005

The regulation and activation of lupus-associated B cells.

Michele L. Fields; Brian D. Hondowicz; Gina N. Wharton; Brigette S. Adair; Michele H. Metzgar; Shawn T. Alexander; Andrew J. Caton; Jan Erikson

Summary:  Anti‐double‐stranded DNA (anti‐dsDNA) B cells are regulated in non‐autoimmune mice. While some are deleted or undergo receptor editing, a population of anti‐dsDNA (VH3H9/Vλ1) B cells that emigrate into the periphery has also been identified. These cells have an altered phenotype relative to normal B cells in that they have a reduced lifespan, appear developmentally arrested, and localize primarily to the T/B‐cell interface in the spleen. This phenotype may be the consequence of immature B cells encountering antigen in the absence of T‐cell help. When provided with T‐cell help, the anti‐dsDNA B cells differentiate into antibody‐forming cells. In the context of the autoimmune‐prone lpr/lpr or gld/gld mutations, the VH3H9/Vλ1 anti‐dsDNA B cells populate the B‐cell follicle and by 12 weeks of age produce serum autoantibodies. The early event of anti‐dsDNA B‐cell follicular entry, in the absence of autoantibody production, is dependent upon CD4+ T cells. We hypothesize that control of autoantibody production in young autoimmune‐prone mice may be regulated by the counterbalancing effect of T‐regulatory (Treg) cells. Consistent with this model, we have demonstrated that Treg cells are able to prevent autoantibody production induced by T‐cell help. Additional studies are aimed at investigating the mechanisms of this suppression as well as probing the impact of distinct forms of T‐cell‐dependent and ‐independent activation on anti‐dsDNA B cells.


Immunologic Research | 2003

The regulation and activation potential of autoreactive B cells

Michele L. Fields; Su-jean Seo; Simone A. Nish; Jeff H. Tsai; Andrew J. Caton; Jan Erikson

Anti-double-stranded DNA (dsDNA) B cells persist even in non-autoimmune-prone animals. In this review, we summarize data regarding the activation potential of these cells. Provision of cognate CD4 T cell help to anti-dsDNA B cells in nonautoimmune mice not only drives their maturation and entry into the B cell follicle, but also leads to secretion of anti-dsDNA autoantibodies. Intriguingly, if T regulatory cells are provided along with T helper cells, the antibody response of anti-dsDNA B cells is diminished. We have also found that T-independent stimulation with CpG oligodeoxy-nucleotides leads to the proliferation and enhanced recovery of anti-dsDNA B cells in vitro. These data suggest that control of anti-dsDNA antibody production may rely on elements from both the innate and adaptive arms of the immune system.


Journal of Autoimmunity | 2008

Autoantibody production in lpr/lpr gld/gld mice reflects accumulation of CD4+ effector cells that are resistant to regulatory T cell activity

Brian D. Hondowicz; Michele L. Fields; Simone A. Nish; Joseph Larkin; Andrew J. Caton; Jan Erikson

In Fas/FasL-deficient mice anti-chromatin Ab production is T cell dependent and is not apparent until after 10 weeks of age. Early control of anti-chromatin antibodies may be due to the counterbalancing influence of Treg cells. Here we show that Treg cells block lpr/lpr gld/gld Th cells from providing help to anti-chromatin B cells in an in vivo transfer system. Interestingly, the percentage and absolute numbers of Foxp3+ Treg cells is elevated in BALB/c-lpr/lpr gld/gld mice and increases with age compared to BALB/c mice. The majority of Foxp3 expression is found in the B220- CD4+ T cell population, and Foxp3-expressing cells are localized in the splenic PALS (periarteriolar lymphocyte sheath). Strikingly, although the lack of functional Fas/FasL does not affect the ability of Treg cells to block Th cell proliferation, Treg cells can block the IFN-gamma differentiation of Th cells from BALB/c or young BALB-lpr/lpr gld/gld mice but not of pre-existing Th1 cells from older BALB/c-lpr/lpr gld/gld mice. Thus, we suggest autoantibody production is not caused by the lack of Treg cells but by a defect in activation-induced cell death that leads to the accumulation of T effector cells that are resistant to regulatory T cell activity.

Collaboration


Dive into the Michele L. Fields's collaboration.

Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar

Laura Mandik-Nayak

Lankenau Institute for Medical Research

View shared research outputs
Top Co-Authors

Avatar

Michael P. Madaio

Georgia Regents University

View shared research outputs
Top Co-Authors

Avatar
Researchain Logo
Decentralizing Knowledge