Michèle Trémolières

Aquatic macrophyte communities as bioindicators of eutrophication in calcareous oligosaprobe stream waters (Upper Rhine plain, Alsace)

The results cover a statistical analysis of the correlations between aquatic macrophyte communities and chemical parameters (N−NH4, N−NO3, P−PO4, COD, Temperature, dissolved O2, Cl) in unpolluted hard waters (upper Rhine rift valley). This study was based on a table of phytosociological relevés for six plant communities, named A, B, C, CD, D and E. The ecological determinism of the communities were defined from: The study of the seven foregoing physico-chemical parameters for 29 groundwater streams on periodical samples of water. The study of the change with time in the aquatic vegetation after change of the trophic status, confirmed by analysis. The comparative study of the vegetation of the streams and parts of the streams with different trophic statuses but fed by the same groundwater table of the Wurmian Rhine gravels. Analysis of the main components showed the good correlation between the macrophyte communities and the trophy (N−NH4, P−PO4). These six communities were classified according to the trophic scale. Discriminant analysis was used to compare the classification of the phytosociological sequence with that based on the statistical analysis. The authors give a very precise bioindication scale (based on the macrophyte community) for the eutrophication degree in unpolluted hard waters.

Impact of river management history on the community structure, species composition and nutrient status in the Rhine alluvial hardwood forest

The present-day Rhine alluvial hardwood forest (Querco-Ulmetum minoris, Issler 24) in the upper Rhine valley (France/Germany) is comprised of three vegetation units, one still flooded by calm waters (F) and the two others unflooded, one for 30 years (UF30) (after the river canalisation) and the other for about 130 years (UF130) (after river straightening and embankment work in the middle nineteenth century). In the three stands, species composition, structure and diversity of vegetation and nutrient content of mature leaf, leaf litter and soil have been studied. Fungi (Macromycetae) were only studied in two stands (F and UF130). The intensity of nutrient recycling was exemplified by comparing the chemical composition of rainwater, flood, throughfall, mature leaf, leaf litter, soil and groundwater in two of these stands (F and UF30).The elimination of floods has caused a change in floristic composition, tree density and plant diversity. Tree density was higher in the two unflooded stands and was related to a large increase in sapling (< 6 cm dbh) density more than to a change of stem (> 6 cm dbh) density. Sapling density increased 2 times and three times in the UF30 and the UF130 respectively, whereas the stem density increased only 12% in the first stand and decreased 29% in the second one. The saprophytic macromycete communities have been supplemented with mycorrhizal species. Leaf litter production was slightly greater in the flooded (4.44 T ha-1 yr-1) than in the two unflooded stands (≤ 3.72 T ha-1 yr-1). Foliar N level is twice as high in the flooded stands in spite of an opposite soil status. P level decreased in soil and leaves with the duration of isolation and was at the same level in the groundwater in two stands (F and UF30). K, Mg and Ca contents of green leaf and leaf litter were high due to the geochemistry of the Rhine substrate (calcareous gravels and pebbles) and similar in all the stands studied, even though there are large inputs of these three elements by floods. Moreover we showed that the groundwater chemistry reflected the variations of nutrient inputs and thus could be a good indicator of the functioning of an alluvial ecosystem and of its change as a result of human activities. The restoration of floods in hardwood forest contributes to the preservation of alluvial vegetational structure and composition, the stimulation of biological processes and a better plant mineral nutrition and water supply.

A reference system for continental running waters: plant communities as bioindicators of increasing eutrophication in alkaline and acidic waters in north-east France

Two bioindication scales of the degree of eutrophication based on aquatic macrophyte communities were established in two types of running waters free of organic matter, the one in acidic “soft” waters (pH 5.5–7.0, conductivity 40–110 μS.cm−1), the other in alkaline hard waters (pH 7–8, conductivity 500–900 μS.cm−1). We show that the main determining factor of the macrophyte distribution is the nutrient level (trophy), especially the level of phosphate and ammonia. The acidic scale, with increasing pH, includes four stages ranging from oligotrophic to eutrophic level (traces to 300 μg.l−1 N-NHinf4p+ and P-PO43−), while the alkaline scale at constant pH comprises six stages of a trophic gradient. For the most part, the floristic composition found in the two sequences is different and depends on conductivity and alkalinity variation. However, some species occur in the two scales and may reflect differences in the trophic level, depending on whether the waters are alkaline or acidic. This change of trophic level for these species is discussed.

The exchange process between river and groundwater on the Central Alsace floodplain (Eastern France)

The recent canalisation (in the nineteen sixties) of the upper Rhine has modified the exchange processes between the river and its groundwater in the floodplain of Alsace. The Rhine seeps through its gravelly bed and in this way feeds the nearby groundwater table by means of the so-called ‘Rhine filtrates’. Using a few groundwater stream examples, this paper presents the characterization and localisation of these infiltrations. The Rhine filtrates are characterized by a high level of chloride and a low level of nitrate, these compounds being hydrological tracers, specific for the Alsatian floodplain (chloride resulting from contamination by the potash mines in the south of Alsace). They are also defined by high levels of phosphate and mercury (very localized injection). Phosphate is responsible for eutrophication which is observable in the appearance of specific aquatic macrophyte communities. Groundwater contamination by mercury is reflected by its accumulation in the bryophyte Fontinalis antipyretica sampled in ground-water streams. Thus aquatic vegetation, and more particularly the distribution of macrophyte communities, is used as an ecological descriptor of the exchange between the Rhine and its groundwater. The maximum injection of Rhine filtrates occurs between two areas of stillwater (hydroelectric dams), where the Rhine dominates its plain and where the substrate is constituted of coarse gravels.

Spatial and temporal variations of nutrient concentration in the groundwater of a floodplain: effect of hydrology, vegetation and substrate

Spatio-temporal variations in nitrogen and phosphorus concentrations in groundwater were analysed and related to the variations in hydrological conditions, vegetation type and substrate in an alluvial ecosystem. This study was conducted in the Illwald forest in the Rhine Plain (eastern France) to assess the removal of nutrients from groundwater in a regularly flooded area. We compared both forest and meadow ecosystems on clayey-silty soils with an anoxic horizon (pseudogley) at 1•5–2 m depth (eutric gley soil) and a forest ecosystem on a clayey-silty fluviosoil rich in organic matter with a gley at 0•5 m depth (calcaric gley soil). Piezometers were used to measure the nutrient concentrations in the groundwater at 2 m depth in the root layer and at 4•5 m depth, below the root layer. Lower concentrations of nitrate and phosphate in groundwater were observed under forest than under meadow, which could be explained by more efficient plant uptake by woody species than herbaceous plants. Thus NO3-N inputs by river floods were reduced by 73% in the shallow groundwater of the forested ecosystem, and only by 37% in the meadow. Compared with the superficial groundwater layer, the lowest level of nitrate nitrogen (NO3-N) and the highest level of ammonium nitrogen (NH4-N) were measured in the deep layer (under the gley horizon at 2•5 m depth), which suggests that the reducing potential of the anoxic horizon in the gley soils contributes to the reduction of nitrate. Nitrate concentrations were higher in the groundwater of the parcel rich in organic matter than in the one poorer in organic matter. Phosphate (PO4-P) concentrations in both shallow and deep groundwater are less than 62 to 76% of those found in surface water which can be related to the retention capacity of the clay colloids of these soils. Moreover, the temporal variations in nutrient concentrations in groundwater are directly related to variations in groundwater level during an annual hydrological cycle. Our results suggest that variations in groundwater level regulate spatio-temporal variations in nutrient concentrations in groundwater as a result of the oxidation–reduction status of soil, which creates favourable or unfavourable conditions for nutrient bioavailability. The hydrological variations are much more important than those concerning substrate and type of vegetation.

Nitrate elimination by denitrification in hardwood forest soils of the Upper Rhine floodplain – correlation with redox potential and organic matter

Denitrification in floodplains is a major issue for river- and groundwater quality. In the Upper Rhine valley, floodplain forests are about to be restored to serve as flood retention areas (polders). Besides flood attenuation in downstream areas, improvement of water quality became recently a major goal for polder construction. Redox potential monitoring was suggested as a means to support assessment of nitrogen elimination in future floodplains by denitrification during controlled flooding. To elucidate the relationship between redox potential and denitrification, experiments with floodplain soils and in situ measurements were done. Floodplain soil of two depth profiles from a hardwood forest of the Upper Rhine valley was incubated anaerobically with continuous nitrate supply. Reduction of nitrate was followed and compared with redox potential and organic matter content. The redox potential under denitrifying conditions ranged from 10 to 300 mV. Redox potential values decreased with increasing nitrate reduction rates and increasing organic matter content. Furthermore, a narrow correlation between organic matter and nitrate reduction was observed. Experiments were intended to help interpreting redox potentials generated under in situ conditions as exemplified by in situ observations for the year 1999. Results obtained by experiments and in situ observations showed that monitoring of redox potential could support management of the flooding regime to optimize nitrogen retention by denitrification in future flood retention areas.

Phosphorus sources for aquatic macrophytes in running waters: water or sediment?

Summary In the Alsace Rhine floodplain, a good relation between the macrophyte communities and the nutrient concentrations in water was established, arguing the importance of the water column in the nutrition of aquatic plants. In this paper, we precise the relative importance of the water column and the sediment in the phosphorus nutrition of three aquatic macrophytes in running water system: Elodea nuttallii, Elodea canadensis, Callitriche obtusangula. We study the relation between the total phosphorus in plant, the phosphorus content in sediment and the phosphate concentrations in water. We show that the annual mean value of phosphorus content in plant is strongly correlated to the annual mean concentration of phosphate in water, for all the three species. In contrast, there is no definite relation between P-plant tissue and the concentration of total P in the sediment.

Regeneration strategies in a temperate hardwood floodplain forest of the Upper Rhine: sexual versus vegetative reproduction of woody species

The regeneration mechanisms of woody species in the hardwood floodplain forest of the Upper Rhine are not well known, although they are of primary importance for future forest development. To gain a better understanding of the balance between sexual and asexual regeneration strategies and to assess the role of the seed bank in regeneration, the similarities in species composition and abundance of four fundamental compartments involved in regeneration (the seed rain, the seed bank, the recruits and the canopy) were compared in three hardwood forest stands with different flooding conditions. The results show that the floristic composition of the recruits is very similar to that of the canopy, whereas the composition of the seed bank is very dissimilar to the latter and comprises very few hardwood species. However, some species such as Fraxinus excelsior, Carpinus betulus and Acer pseudoplatanus which are very rare in the seed bank release a high number of diaspores, and seedlings of these species germinate abundantly in the field in the year following seed release. Moreover, the germinating seeds originate from the litter layer and not from the soil itself. This shows that most woody species regenerating by seed in the hardwood Rhine forest build transient seed banks and that the role of persistent seed banks for regeneration is very limited. Furthermore, it appears that many woody species have developed strategies favouring vegetative propagation for their regeneration, particularly understorey species, such as Cornus sanguinea and Prunus padus. As vegetatively grown individuals better withstand prolonged inundation in their early life stages than seedlings, species relying on vegetative regeneration strategies might be advantaged by regular and prolonged flooding of the Rhine forest over species regenerating only by seeds.

Aquatic macrophytes as bioindicators of carbon dioxide in groundwater fed rivers.

Aquatic plants have been used as hydrological tracers in groundwater fed river systems. In nature, patterns in plant distribution have been attributed to ammonium (NH(4)) toxicity and phosphate (PO(4)) limitation, while some laboratory studies have focused on the role of the partial pressure of CO(2) (pCO(2)). The aims of this study were (i) to test whether plant distribution was more related to pCO(2) than NH(4) and PO(4) in nature, (ii) to develop and test the predictive power of new plant indices for pCO(2), NH(4) and PO(4), and (iii) to test the potential causality of the relationships using species eco-physiological traits. These tests were carried out with field data from the Rhine, Rhône and Danube river basins. Species composition was best related to the effect of pCO(2). The pCO(2) plant index was well calibrated (r(2)=0.73) and had the best predictive power (r(2)=0.47) of the three indices tested on independent datasets. The plant-pCO(2) relationship was supported by a biological mechanism: the ability of strictly submerged species of aquatic vascular plants to use HCO(3) under low pCO(2). This was not the whole story: the effects of pCO(2), NH(4) and PO(4) on plant distribution were partially confounded and interacted all together with temperature. However, neither NH(4) toxicity nor P limitation could be asserted using species eco-physiological traits. Moreover, the predictive power of the NH(4) and PO(4) plant indices was not as strong as pCO(2), at r(2)=0.24 and r(2)=0.27, respectively. Other potentially confounding variables such as spatial structure, biotic and physical factors were unlikely to confound the findings of this study.

Effect of ammonium ions on the net photosynthesis of three species of Elodea

Abstract Three species of Elodea, E. canadensis Michx., E. ernstae St John and E. nuttallii St John were contaminated with concentrations of NH 4 + occurring in the environment at 0.25, 0.50 and 1 mg l −1 and also with concentrations, clearly greater than natural values, of 2.5., 5.0 and 7.5 mg l −1 . These assays were carried out over a 7 day test period. The effects of ammonium were investigated by measuring the net photosynthesis and residue analyses of the active substance in water were also carried out. Three different significant types of reaction were found: (1) strong concentrations of ammonium nitrogen (1–7.5 mg l −1 of N-NH 4 + ) do not affect the photosynthetic activity of E. ernstae ; (2) these concentrations stimulate the photosynthesis of E. nuttallii ; (3) they inhibit the photosynthesis of E. canadensis .