Michio Mito

Developmental expression of cytochrome P450S within intrasplenically transplanted fetal hepatocytes.

Fetal hepatocytes were harvested at day 20 of gestation from spontaneously hypertensive rats (SHR) and then transplanted into recipient adult SHR spleens. Morphological examination of the recipient spleens revealed that, after 4 and 10 wk, large masses of hepatocytes were present in the red pulp with apparent cord-like structures. Larger batches of hepatocytes were observed in the spleens at 10 wk after than at 4 wk after transplantation. Of major significance was the fact that hepatocyte transplanted spleens were able to express several families of cytochrome P450 (cyto P450) proteins 2-10 wk after transplantation. Immunochemical determinations revealed that cytos P450 IA1, P450 IIB1, P450 p, P450 HLp, and P450 LA omega could be detected without any prior induction. All were intensely expressed 6 wk after transplantation; however, P450 IA1 and P450 IIB1 did not appear to be expressed by 2 wk after transplantation. Although cytos P450 p and P450 HLp did not appear to be expressed by 10 wk after transplantation, they were induced with dexamethasone at that time. Cyto P450 LA omega and peroxisomal acyl CoA oxidase were expressed 6 wk after transplantation in a 70% hepatectomized host. These results demonstrate that fetal hepatocytes can be successfully transplanted into the spleens of recipients and that the fetal hepatocytes appear to grow and develop cyto P450 metabolizing systems.

A Case Report of Malignant Lymphoma of the Stomach with Difficulty of Differential Diagnosis with RLH.

胃悪性リンパ腫は胃悪性腫瘍の1%とされまれな疾患とされる.今回われわれは胃反応性リンパ細網細胞増生reactive lymphoreticular hyperplasia (RLH) との鑑別が非常に困難であり, また術前にherpes zoster脳炎も併発した興味ある症例を経験したので報告する.76歳の男性で主訴は心窩部痛, 胃X線造影, 胃内視鏡, 腹部CT検査にて胃の粘膜下腫瘍病変が発見された.生検材料における病理組織学的および免疫組織学的所見では, RLHと診断されるもlow grade malignancyの悪性リンパ腫も否定できず手術を施行した.切除標本病理の組織および免疫組織学的診断は, RLHを随伴する胃悪性リンパ腫 (diffuse lymphoma, small cell type, Bcell pheno type) であり, RLHが胃悪性リンパ腫の初期像であることを示唆する症例と考えられた.