Michiyasu Sugii

A new radiometric determination of l-canaline

Abstract A new radiometric determination of l -canaline is described in which [2-14C]acetone is employed as a reagent. The reagent is volatile while the reaction product of the reagent with l -canaline is not. Unreacted reagent is removed in vacuo; the residue radioactivity is proportional to the l -canaline content. The major advantages of this assay are specificity and sensitivity.

Change of Atropine Esterase Activity in the Regenerated Plants of Duboisia myoporoides during Development, and its Relation to Alkaloid Accumulation

Summary Atropine esterase activity in the regenerated plants of Duboisia myoporoides was determined during development and compared with that of seedlings. High esterase activity was found in the roots of seedlings throughout growing, whereas the activity in the roots of regenerated plants was extremely low at the early stages of development, though after regeneration when they were 6-months old the activity was recovered at the levels found in seedlings. The relationship between tropane alkaloid content in the leaves and atropine esterase activity in the roots of regenerated plantlets was examined. The results showed that extremely low activity in the roots seemed to be due to no or low accumulation of alkaloids in the leaves. Alkaloid dynamics in D. myoporoides were discussed.

Esterification of tropine in cultured tissues of duboisia myoporoides

Abstract Tropine was converted into acetyltropine in callus cultures Duboisia myoporoides , while in shoot cultures butropine was found as an esterified product.