Miguel Angel Pérez Nieto

Gastric Cancer Susceptibility Is Not Linked to Pro- and Anti-Inflammatory Cytokine Gene Polymorphisms in Whites: A Nationwide Multicenter Study in Spain

BACKGROUND AND AIMS:Recent studies have reported an association between cytokine gene polymorphisms and GC risk. However, results are inconsistent among studies from different geographic regions and ethnic groups. Our goal was to evaluate the influence of Helicobacter pylori (H. pylori) infection and host genetic factors on GC susceptibility in a population of Spanish white GC patients.METHODS: DNA from 404 unrelated patients with GC and 404 sex- and age-matched healthy controls was typed for several functional polymorphisms in pro- (IL-1B, TNFA, LTA, IL-12p40) and anti-inflammatory (IL-4, IL-1RN, IL-10, TGFB1) genes by PCR, RFLP, and TaqMan assays. H. pylori infection and CagA/VacA antibody status were also determined by western blot serology.RESULTS: Logistic regression analysis identified H. pylori infection with cagA strains (OR 2.54, 95% CI 1.77–3.66), smoking habit (OR 1.91, 95% CI 1.25–2.93), and positive family history of GC (OR 3.67, 95% CI 2.01–6.71) as independent risk factors for GC. None of the cytokine gene polymorphisms analyzed in this study were associated with susceptibility to GC development, whether GC patients were analyzed as a group or categorized according to anatomic location or histological subtype. Some simultaneous combinations of proinflammatory genotypes reportedly associated with greater GC risk yielded no significant differences between patients and controls.CONCLUSIONS: Our results show that, at least in some white populations, the contribution of the cytokine gene polymorphisms evaluated in this study (IL-1B, IL-1RN, IL-12p40, LTA, IL-10, IL-4, and TGF-B1) to GC susceptibility may be less relevant than previously reported.

Psychometric Properties of the Spanish Adaptation of the Anger Rumination Scale: Evidence of Reliability and Validity in the General Population.

The key role of rumination and perseveration processes in anger experience has been empirically supported. The tendency to ruminate has been demonstrated to be crucial in understanding pathological and adaptive behaviours. The Anger Rumination Scale (ARS) was developed to assess anger rumination frequency when people are angry, showing adequate levels of reliability and validity. However, although it has been adapted to several languages, the development of the Spanish version was still pending. The aim of this study, therefore, was to develop the Spanish adaptation of the ARS and to validate it in a general population sample (N = 388). Participants were asked to complete the ARS as well as other measures of anger (STAXI-2), anxiety (STAI-T), depression (BDI-II short form), rumination (PSWQ), and thought self-regulation (TCQ). A confirmatory factor analysis replicated the four-factor structure obtained with the original version (S-B χ2(145) = 323.26, p < .00005; CFI = .92; TLI = .90; RMSEA = .06; SRMR = .05). The resulting subscales (i.e., Angry Afterthoughts, Angry Memories, Thoughts of Revenge, and Understanding of Causes) met psychometric criteria of reliability (α = .89) and validity. In conclusion, a psychometrically sound Spanish adaptation of the ARS is now available.

S1946 Relevance of GSTM1, GSTT1, and GSTP1 Gene Polymorphisms On Gastric Cancer Susceptibility

Relevance of GSTM1, GSTT1, and GSTP1 Gene Polymorphisms On Gastric Cancer Susceptibility Maria Asuncion Garcia-Gonzalez, Enrique Quintero, Rafael Benito, Mark Strunk, David Nicolas-Perez, Adolfo Parra-Blanco, Santos Santolaria, Federico Sopena, Elena Piazuelo, Pilar Jimenez, Maria Badia, Jesus Espinel, Rafael Campo, Marisa Manzano, Fernando Geijo, Maria Pellise, Ferran Gonzalez-Huix, Miguel Nieto, Jorge C. Espinos, LLucia Tito, Luis Bujanda, Manuel Zaballa, Maria Angeles Perez-Aisa, Isabel Maria Mendez-Sanchez, Angel Lanas