Mikhail Kashlev

RNA Polymerase Switches between Inactivated and Activated States By Translocating Back and Forth along the DNA and the RNA

Important regulatory events in both prokaryotic and eukaryotic transcription are currently explained in terms of an inchworming model of elongation. In this model, RNA extension is carried out by a mobile catalytic center that, at certain DNA sites, advances within stationary RNA polymerase. This idea emerged from the observation that footprints of individual elongation complexes, haltedin vitro at consecutive DNA positions, can remain fixed on the template for several contiguous nucleotide additions. Here, we examine in detail the structural transitions that occur immediately after the enzyme stops at sites where discontinuous advancement of RNA polymerase is observed. We demonstrate that halting at such special sites does not “freeze” RNA polymerase at one location but induces it to leave its initial position and to slide backward along the DNA and the RNA without degrading the transcript. The resulting loss of contact between the RNA 3′-hydroxyl and the enzyme’s catalytic center leads to temporary loss of the catalytic activity. This process is equilibrated with enzyme return to the original location, so that RNA polymerase is envisaged as an oscillating object switching between catalytically active and inactive states. The retreated isoform constitutes a principal intermediate in factor-induced endonucleolytic RNA cleavage. These oscillations of RNA polymerase can explain its apparent discontinuous advancement, which had been interpreted as indicating flexibility within the enzyme.

Mapping of Catalytic Residues in the RNA Polymerase Active Center

When the Mg2+ ion in the catalytic center of Escherichia coli RNA polymerase (RNAP) is replaced with Fe2+, hydroxyl radicals are generated. In the promoter complex, such radicals cleave template DNA near the transcription start site, whereas the β′ subunit is cleaved at a conserved motif NADFDGD (Asn-Ala-Asp-Phe-Asp-Gly-Asp). Substitution of the three aspartate residues with alanine creates a dominant lethal mutation. The mutant RNAP is catalytically inactive but can bind promoters and form an open complex. The mutant fails to support Fe2+-induced cleavage of DNA or protein. Thus, the NADFDGD motif is involved in chelation of the active center Mg2+.

Coupling between transcription termination and RNA polymerase inchworming

Advancement of RNA polymerase of E. coli occurs in alternating laps of monotonic and inchworm-like movement. Cycles of inchworming are encoded in DNA and involve straining and relaxation of the ternary complex accompanied by characteristic leaping of DNA and RNA footprints. We demonstrate that the oligo(T) tract that constitutes a normal part of transcription terminators acts as an inchworming signal so that the leap coincides with the termination event. Prevention of leaping with a roadblock of cleavage-defective EcoRI protein results in suppression of RNA chain release at a termination site. The results indicate that straining and relaxation of RNA polymerase are steps in the termination mechanism.

Molecular cloning and characterization of Borrelia burgdorferi rpoB

Borrelia burgdorferi rpoB, the gene encoding the beta-subunit of RNA polymerase, has been cloned and sequenced. The full-length gene encodes a protein of 1154 amino acids with a calculated molecular mass of 129.8 kDa. The amino-acid sequence is 49% identical to the corresponding protein from Escherichia coli. B. burgdorferi rpoB is a component of a gene cluster, which includes rplJ, rplL and rpoC. A temperature-sensitive E. coli rpoB mutant could be complemented by introduction of the B. burgdorferi gene, indicating that the B. burgdorferi rpoB is expressed in E. coli and the beta-subunit can be assembled into functional holoenzyme. The wild-type amino-acid sequence of the B. burgdorferi beta-subunit is consistent with those of spontaneously arising rifampicin-resistant mutants of E. coli and Mycobacterium tuberculosis at certain critical residues. This suggests that the natural resistance of B. burgdorferi to rifampicin may be due to the primary amino-acid sequence of its beta-subunit.

Strategies and Methods of Cross-Linking of RNA Polymerase Active Center

Publisher Summary This chapter presents the basic strategies and methods of cross-linking of RNA polymerase (RNAP) active center. In order to achieve the high selectivity of affinity labeling for RNA polymerase, it is required to take advantage of “catalytic competence.” This phenomenon reflects the ability of a substrate residue cross-linked at the active center of an enzyme to convert to a cross-linked product by the same enzyme molecule according to the normal mechanism of catalysis. At the first stage RNAP is treated in the binary complex with a promoter by affinity reagent, which is an analog of initiating substrate. This results in the cross-linking of affinity reagent residues both inside and outside the active center. At the second stage the modified enzyme is supplemented with the second radioactive substrate complementary to the next base of DNA template. The chapter discusses RNA–protein cross-linking in the active center of initiating and elongation. Single-Hit degradation of polypeptides at particular residues is also discussed.. Multiple cross-linking sites can be revealed by quantitative analysis of single-hit degradation products. Another approach that appears to be very helpful for the mapping is based on the usage of functionally active enzymes assembled from the fragments of RNAP subunits.

Stability of the pBR322 plasmid as affected by the promoter region of the tetracycline-resistance gene

A region affecting the pBR322 plasmid maintenance has been located within the region of the TcR gene promoter. On the basis of stability analysis of pBR322 derivatives comprising the modified region of the TcR gene, we deduced that it is the nucleotide sequence localized in the region of the HindIII site that causes destabilization of the plasmid and not the TcR gene product or active transcription of this region. The destabilizing effect is manifested both in cis and in trans.