Miklós Réthelyi

Cell and neuropil architecture of the intermediolateral (sympathetic) nucleus of cat spinal cord

Abstract A Golgi study of the neuronal elements in the thoracic intermedio-lateral nucleus (preganglionic sympathetic neuronal column) is presented, with some additional electron microscopic observations. The dendrites of the sympathetic neurons are strictly longitudinally oriented; their axons can be traced along the border between the ventral horn and the lateral funiculus directly into the ventral rootlers. No initial collateral of the axons can be observed. The neuronal population of the intermedio-lateral nucleus is entirely homogeneous and little contaminated by dendrites of other cells. The axonal neuropil of the nucleus is also longitudinally oriented and is fed mainly from collaterals of the lateral funiculus. The synaptic arrangement between the axonal and dendritic arborizations is mainly of the ‘climbing type’. Three different kinds of synaptic terminals can be recognized in the electron microscope picture: (1) terminals containing an unusually large number of large osmiophilic vesicles and apparently clear ordinary vesicles; (2) terminals (often axo-somatic) containing characteristic flattened vesicles; and (3) axo-dendritic terminals containing clear spherical synaptic vesicles.

Is the knife-cut in the hypothalamus a permanent barrier to regrowth of nerve fibers?-an affirmative answer

The regenerative capacity of nerve fibers was studied in adult female rats. Horseradish peroxidase was injected into the anterior hypothalamus lateral to the suprachiasmatic nucleus 4 days, 6 weeks and 4 months, respectively, following an archiform retrochiasmatic knife-cut. The trajectory of the stained fibers was examined on horizontal sections of the hypothalamus. No nerve fibers could be seen sprouting across the scar-tissue of the knife-cut regardless of the survival time. In one rat (6-week survival time) a bundle of fine nerve fibers turned in a medial direction at the caudal end of the knife-cut, suggesting that sprouting fibers were destined to reinnervate parts of the deafferented medial-basal hypothalamus.

Cocaine- and amphetamine-regulated transcript peptide (CART) is present in peptidergic C primary afferents and axons of excitatory interneurons with a possible role in nociception in the superficial laminae of the rat spinal cord

Cocaine‐ and amphetamine‐regulated transcript peptides (CART) have been implicated in the regulation of several physiological functions, including pain transmission. A dense plexus of CART‐immunoreactive fibres has been described in the superficial laminae of the spinal cord, which are key areas in sensory information and pain processing. In this study, we used antibody against CART peptide, together with markers for various types of primary afferents, interneurons and descending systems to determine the origin of the CART‐immunoreactive axons in the superficial laminae of the rat spinal cord. Calcitonin gene‐related peptide (CGRP), a marker for peptidergic primary afferents in the dorsal horn, was present in 72.6% and 34.8% of CART‐immunoreactive axons in lamina I and II, respectively. The majority of these fibres also contained substance P (SP), while a few were somatostatin (SOM)‐positive. The other subpopulation of CART‐immunoreactive boutons in lamina I and II also expressed SP and/or SOM without CGRP, but contained vesicular glutamate transporter 2, which is present mainly in excitatory interneuronal terminals. Our data demonstrate that the majority of CART‐immunoreactive axons in the spinal dorsal horn originate from peptidergic nociceptive primary afferents, while the rest arise from excitatory interneurons that contain SP or SOM. This strongly suggests that CART peptide can affect glutamatergic neurotransmission as well as the release and effects of SP and SOM in nociception and other sensory processes.

The caudal end of the rat spinal cord: transformation to and ultrastructure of the filum terminale.

Contrary to the current belief, the spinal cord of the rat does not terminate with the conus terminalis (CT), but its basic components (central canal, gray matter, white matter) continue in the filum terminale (FT). Proceeding caudally in the conus terminalis, first the motoneuron cell column discontinues in the ventral horn. More caudally the dorsal horns separate from the intermediate zone, and discontinue. The ensuing filum terminale consists of the slit-like central canal lined by ciliated ependymal cells, the periventricular gray matter and the peripheral white matter. Uniform small size neurons and glial cells populate the gray matter. Ultrastructural analysis revealed various types of axodendritic and axosomatic synapses as well as fine unmyelinated axons. The white matter consists mainly of myelinated nerve fibers. The neuronal components of the filum terminale, if they occur also in the human spinal cord, should be involved in the diagnosis and treatment of various diseases, e.g. tethered spinal cord syndrome, vascular malformations and disraphysm.

Lamellar Arrangement of Neuronal Somata in the Dorsal Root Ganglion of the Cat

The retrograde transport of horseradish peroxidase (HRP) was used to study the distribution of perikarya in the dorsal root ganglia (DRGs). Injections of HRP subcutaneously into a small area of the foreleg, flank, perineum, the central pad of the forepaw, muscles of the foreleg, the wall of the urinary bladder, and mucosa of the rectum resulted in many retrogradely labeled perikarya in one DRG. Labeled perikarya were distributed in the ganglia proximally to distal elongated slabs or columns, especially in cases of subcutaneous injections. A similar slab, or columnar distribution, of HRP-labeled perikarya was noticed when the tracer was injected into the spinal cord preceded by the transection of all dorsal root filaments but one. Perikarya located along the lateral border of the ganglion were labeled through rostral filaments, and perikarya distributed along the medial border were labeled through caudal filaments. A segmental somatotopic map has been conceived for the DRG as an intermediate territory between the periphery and the spinal cord.

A system of intraependymal cisternae along the margins of the median eminence in the rat: structure, three-dimensional arrangement and ontogeny.

SummaryStructure, three-dimensional arrangement and ontogeny of large intracellular cisternae located in the median eminence region of the rat hypothalamus were studied using toluidin-blue stained semithin sections and electron microscopy. The cisternae occur along the projections of ependymal cells lining the ventral portion of the third ventricle (infundibular recess). Small cisternae can be seen close to the ventricle, whereas larger ones, divided into smaller compartments by thin septa, cluster near the surface of the hypothalamus. The cisternae are encompassed by a thin layer of cytoplasm to which axon terminals containing synaptic and dense core vesicles are closely related. Cisternae are arranged around the median eminence in a characteristic pattern. They occupy the midline in the retrochiasmatic area, flank both margins of the median eminence and extend caudally behind the origin of the pituitary stalk. The cisternae appear first between the 15th and 17th postnatal days. At about the 30th day their size and distribution resemble the situation observed in adult animals. The ependymal cisternae are suggested to be closely related to the luteinizing-hormone releasing-hormone (LH-RH)-containing fibers.

Synapses upon the axon origin of dorsal horn neurons in the rat spinal cord.

Axon terminals synapsing with axon hillocks or origins of Golgi-impregnated and gold-toned neurons in the dorsal horn of the rat were shown in serial electron micrographs. Synapses occurred irrespective of the site (perikaryon or dendrite) and mode (with or without an axon hillock) of the axon origin. The synapsing axon terminals contained 3 populations of vesicles: pleomorphic and flattened synaptic vesicles and a combination of pleomorphic and dense-core vesicles. The membrane thickening in the axon-axon hillock synapses was of the symmetrical type.

Caudal end of the rat spinal dorsal horn

We have previously demonstrated that the transformation of the caudal spinal cord through the conus medullaris to the filum terminale takes place in three steps. In the conus medullaris the twin layers of CGRP-immunoreactive and IB4-labeled primary afferent fibers as well as the translucent portion of the superficial dorsal horn equivalent to the substantia gelatinosa discontinue before the complete removal of the dorsal horn. Parallel with these changes VGLUT1-immunoreactive myelinated primary afferent fibers arborize not only in the deep layers but also in the entire extension of the remaining dorsal horn, while scattered CGRP fibers still remains at the margin of and deep in the dorsal horn. PKCgamma-immunoreactive dorsal horn neurons discontinue parallel with the disappearance of the IB4-labeled nerve fibers. These observations suggest that in the dorsal horn certain neurons are linked to the substantia gelatinosa, while others are substantia gelatinosa-independent neurons.