Mila Maidarti

Ovarian tissue vitrification as a method for fertility preservation: A study of follicle number and morphology after vitrification

Purpose: The aim was to evaluate the effects of ovarian cortex vitrification on follicle number and morphology. Materials and Methods: We excised approximately 5 mm of ovarian tissue from one ovary of each of 10 reproductive aged women who underwent surgery for the removal of benign ovarian tumors. A cryoprotectant agent containing 7.5% dimethylsulphoxide and 7.5% ethylene glycol was added to the ovarian cortex. Post-vitrification ovarian cortex was placed in a Cryotissue device and frozen in liquid nitrogen. We evaluated the shape and number of the follicles as well as the granulosa cell layers. The quality of the follicles was evaluated based on the morphological integrity of the oocytes, granulosa cells, and basal membranes as determined by hematoxylin and eosin staining. Results: The median age of the patients was 33 years (range: 28-40 years). The mean number of follicles per patient was 17 ± 7. After thawing, 59 primordial follicles (35.3%), 45 (26.9%) primary follicles and 6 (3.5%) secondary follicles were retrieved. The remaining follicles (34.1%) were atretic. There was a significant relationship between number of follicles and age ( P = 0.012, r = −0751). No differences were found in the morphology of the granulosa cells, stroma or collagen in both groups. The number of primordial follicles, primary follicles, secondary follicles, and follicular atresia were not significantly different before and after vitrification. Conclusion: In our study, vitrification does not cause significant changes to the morphology and number of follicles.

Indonesian kalkulator of oocytes (IKO): A smart application to determine our biological age

Background: The use of smartphones and its associated application provides new opportunities for physicians. In current situations, there are still few applications are designed in the field of infertility and Assisted Reproductive Technologies (ART). A study conducted on 1616 subjects proved that AMH (Anti-Mullerian Hormone) could be used to predict a woman’s biological age earlier than Follicle-Stimulating Hormone (FSH) and Antral Follicle Count (AFC). In this study, we describe the AMH nomogram that has been developed into a mobile application as “Indonesian Kalculator of Oocytes” (IKO). The software required to create IKO application was the Android 4.0.3 Ice Cream Sandwich and Java Application Development. The hardware specification that needed to develop the IKO apps were a 4.0-inch screen, 512 MB RAM (random-access memory), and CPU (central processing unit) with dual core 1.2 Ghz. The application is built using the Android SDK (Software Development Kit) and Java Application Development. In this app...

An evaluation of multimedia and online support groups (OSG) contents and application of information by infertile patients: Mixed method study

Background: The presence of Online Support Groups (OSG) is expected to empower patients with infertility, thus allowing patients to be the focus of healthcare services. This study will evaluate multimedia content, OSG, and utilization of information for decision-making by patients using infertility services. This study is a mixed method study conducted from January – June 2016 at Yasmin IVF Clinic, Dr. Cipto Mangunkusumo General Hospital; and SMART IVF Clinic, Jakarta. The subjects are patients with infertility who sought treatment at the clinics. Data was collected through a structured interview in the form of a questionnaire. Informed consent was obtained from all individual participants included in the study. All procedures that performed in studies were by the ethical standards of the institutional. The result from 72 respondents showed quantitative analysis did not reveal any association between multimedia and OSG information sources with patient knowledge regarding infertility management. However, q...

Expression of CA-125 Level And Neutrophil to Lymphocyte Ratio In Infected And Non-infected Endometrioma

Implantation and growth of endometriosis was determined by immune cell. There were several immunologic cells that promoting implantation and cell proliferation such as macrophages, Natural killer, lymphocyte and monocyte. Infected endometrioma was associated in women with revised American Society for Reproductive Medicine (ASRM) stage III-IV. Neutrophil lymphocyte ratio (NLR) was a simple systemic inflammation response markers. The sensitivity and spesificity CA-125 in predicting endometrioma was very low but it had been used to monitor the progress of endometriosis. Therefore, measuring mean in leucocyte, NLR, PLR and CA-125 level in infected endometrioma was necessary.