Milan Novak

Relation of adrenal cortical steroids to antibody release.

Conclusions 1. The anamnestic reaction was produced by means of subcutaneously administered adrenal cortical steroids in oil to rabbits immunized against sheep erythrocytes. 2. Lymphocytes obtained from the appendix, thymus and mesenteric node of rabbits when injected intravenously into a normal rabbit circulate for a period of about 30 minutes only. 3. Lymphocytes obtained from the appendix, thymus and mesenteric node of rabbits immunized against sheep erythrocytes when injected intravenously into a normal rabbit do not release antibody under the conditions of the experimental method employed. 4. Adrenal cortical steroids administered to a normal rabbit which also receives lymphocytes intravenously from rabbits immunized against sheep erythrocytes do not induce a release of antibody from these cells while they are circulating according to the experimental method employed. The authors are indebted to the Upjohn Co., Kalamazoo, Mich., for 40 cc of the 80 cc of Lipo-Adrenal Cortex used in the experiment.

Tetanus Prophylaxis with Penicillin-Procaine G.

Conclusions Results indicate that penicillin-procaine G in sesame oil and 2% aluminum monostearate is of significant value prophylactically in lowering the mortality of mice experimentally infected with a lethal dose of detoxified spores of Clostridium tetani. In addition to decreasing the mortality, it retards the development of symptoms and resulting deaths. Injected into the necrotic areas, it is more effective than the same unitage injected at an uninfectd site. Whether this is caused by insufficient penetration of the drug due to the presence of necrotic tissue or to interference by the calcium chloride has not been determined.

Bactericidal activity of phagocytin from rabbits exposed to whole body x-radiation.

Summary Peritoneal exudates were induced in female rabbits and collected either 1, 5 or 30 days after total body exposure to 400 r and 1 or 5 days after 500 r. Exudate cells from unirradiated rabbits served as controls. Phagocytin was extracted from the exudate cells (heterophils) and tested for bactericidal activity against Escherichia colt, Salmonella enteritidis, Klebsiella pneumoniae and Pseudomonas aeruginosa. Phagocytin from cells collected 1 day after exposure to 400 or 500 r and 5 days after exposure to 500 r showed a decreased activity against all the test micro-organisms. Phagocytin from cells collected 5 days after exposure to 400 r showed only a slight change in activity from controls. A marked increase in phagocytin activity was observed in extracts of cells collected 30 days after exposure to 400 r. The authors are indebted to Dr. W. S. Moos, Dr. J. W. Yusken and Mr. R. Haas, Radiology Dept., College of Medicine, Univ. of Illinois, for the dosimetry and to Miss Mabel Counelis for irradiating the rabbits.

Antibacterial Properties of Some Positive Univalent Iodine Compounds

Summary From these experiments, one can conclude that monopyridine iodine (I) p-nitrobenzoate rapidly destroys common pathogenic bacteria and that it is relatively non-toxic and non-irritating