Milica Ljaljevic-Grbic
University of Belgrade
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Featured researches published by Milica Ljaljevic-Grbic.
The Science of Nature | 2017
Marija Nenadić; Milica Ljaljevic-Grbic; Miloš Stupar; Jelena Vukojević; Ana Ćirić; Vele Tešević; Ljubodrag Vujisić; Marina Todosijević; Nikola Vesović; Nemanja Živković; Srećko Ćurčić
The antifungal potential of the pygidial gland secretion of the troglophilic ground beetle Laemostenus punctatus from a cave in Southeastern Serbia against cave-dwelling micromycetes, isolated from the same habitat, has been investigated. Eleven collected samples were analyzed and 32 isolates of cave-dwelling fungi were documented. A total of 14 fungal species were identified as members of the genera Aspergillus, Penicillium, Alternaria, Cladosporium, Rhizopus, Trichoderma, Arthrinium, Aureobasidium, Epicoccum, Talaromyces, and Fusarium. Five isolates were selected for testing the antifungal activity of the pygidial gland secretion: Talaromyces duclauxi, Aspergillus brunneouniseriatus, Penicillium sp., Rhizopus stolonifer, and Trichoderma viride. The microdilution method has been applied to detect minimal inhibitory concentrations (MICs) and minimal fungicidal concentrations (MFCs). The most sensitive isolate was Penicillium sp., while the other isolates demonstrated a high level of resistance to the tested agent. L. punctatus has developed a special mechanism of producing specific compounds that act synergistically within the secretion mixture, which are responsible for the antifungal action against pathogens from the cave. The results open opportunities for further research in the field of ground beetle defense against pathogens, which could have an important application in human medicine, in addition to the environmental impact, primarily.
Drug Development and Industrial Pharmacy | 2015
Filipa S. Reis; Ana Ćirić; Dejan Stojković; Lillian Barros; Milica Ljaljevic-Grbic; Marina Soković; Isabel C.F.R. Ferreira
Abstract The genus Penicillium is well known for its importance in drug and food production. Certain species are produced on an industrial scale for the production of antibiotics (e.g. penicillin) or for insertion in food (e.g. cheese). In the present work, three Penicillium species, part of the natural mycobiota growing on various food products were selected – P. ochrochloron, P. funiculosum and P. verrucosum var. cyclopium. The objective of our study was to value these species from the point of view of production of bioactive metabolites. The species were obtained after inoculation and growth in Czapek and Malt media. Both mycelia and culture media were analyzed to monitor the production of different metabolites by each fungus and their release to the culture medium. The concentrations of sugars, organic acids, phenolic acids and tocopherols were determined. Antioxidant activity of the phenolic extracts was evaluated, as also the antimicrobial activity of phenolic acids, organic acids and tocopherols extracts. Rhamnose, xylose, fructose and trehalose were found in all the mycelia and culture media; the prevailing organic acids were oxalic and fumaric acids, and protocatechuic and p-hydroxybenzoic acids were the most common phenolic acids; γ-tocopherol was the most abundant vitamin E isoform. Generally, the phenolic extracts corresponding to the mycelia samples revealed higher antioxidant activity. Concerning the antimicrobial activity there were some fluctuations, however all the studied species revealed activity against the tested strains. Therefore, the in-vitro bioprocesses can be an alternative for the production of bioactive metabolites that can be used by pharmaceutical industry.
Zbornik Matice Srpske Za Prirodne Nauke | 2005
Milica Ljaljevic-Grbic; Jelena Vukojević; Jasmina Glamočlija; Dušica A. Janošević; Dragoljub Grubišić; Jelena T. Lević
Yellow pheasant´s Eye is a herbaceous plant from dry ressy areas. Owing to habitat destruction and over- collection for ornamental and medical purposes A. vernalis L. has became scarce in central and south Europe. The reasons for A. vernalis threatened are manyfold. The low seeds germination rate is significant. According to our investigation the main cause of fruit destruction is fungal infection. From the surface of the fruits, collected in Deliblato Sands, the following micromycetes has been isolated and determinated: Fusarium solani (Mart) Sacc., Fusarium sporotrichioides Sherb., Alternaria sp. and Drechslera sp. Histologycal analysis showed the presence of conidiomata and conidia Phoma sp. in the seeds.
Archives of Biological Sciences | 2010
Milica Ljaljevic-Grbic; Jelena Vukojević; Gordana Subakov-Simić; Jelena Krizmanić; Miloš Stupar
Archives of Biological Sciences | 2013
Milica Ljaljevic-Grbic; Miloš Stupar; Jelena Vukojević; Ivana Maricic; Natasa Bungur
Archives of Biological Sciences | 2008
Milica Ljaljevic-Grbic; Jelena Vukojević; Miloš Stupar
Zbornik Matice Srpske Za Prirodne Nauke | 2009
Milica Ljaljevic-Grbic; Jelena Vukojević
Archives of Biological Sciences | 2008
Marija Marin; Snezana Budimir; Dušica Janošević; Petar D. Marin; Sonja Duletić-Laušević; Milica Ljaljevic-Grbic
Archives of Biological Sciences | 2014
Miloš Stupar; Milica Ljaljevic-Grbic; Ana Dzamic; Nikola Unković; Mihailo S. Ristić; Jelena Vukojević
Archives of Biological Sciences | 2011
Milica Ljaljevic-Grbic; Miloš Stupar; Jelena Vukojević; Dragoljub Grubišić