Milica Pavlović

Essential oil and its systematic significance in species of Micromeria Bentham from Serbia & Montenegro

The composition and quantity of the essential oil of Micromeria allowed us to clearly distinguish between sections Pseudomelissa and Eumicromeria. According to our results the species of section Pseudomelissa (M. thymifolia, M. albanica, M. dalmatica and M. pulegium) contained a large quantity of oil (>0.5%) dominated by oxygenated monoterpenes of the menthane type, while the species of section Eumicromeria (M. croatica, M. juliana, M. cristata and M. parviflora) had a small quantity of essential oil (<0.5%) dominated by various terpene compounds. 0.5% of essential oil is defined like boundary value on the basis of the lowest quantity of essential oil measured in populations of species of section Pseudomelissa.

Antimicrobial and antioxidant properties of methanol extracts of two Athamanta turbith subspecies

The methanol extracts of fruits and leaves of Athamanta turbith subsp. hungarica (Borbás) Tutin (Umbelliferae) and A. turbith subsp. haynaldii (Borbás & Uechtr.) Tutin were analyzed for their antimicrobial and antioxidant properties. Phenolic compounds (flavonoids and phenolic acids) of the extracts were examined using HPLC. All the extracts were characterized by the presence of caffeic acid derivates, luteolin and its glycosides, with luteolin 7-O-glucoside as one of the main compounds. Luteolin 7-O-rutinoside was detected only in A. turbith subsp. haynaldii extracts. Investigation of antimicrobial activity was performed against six bacteria and two fungal strains, using the agar diffusion technique and broth microdilution assay. The extracts of investigated A. turbith subspecies exerted similar antimicrobial activity, whereas the best activity was detected against Candida albicans. In order to investigate antioxidant properties, ferric ion reducing antioxidant power (FRAP), radical scavenging capacity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl radical (HO·), and the effect on lipid peroxidation (LP) were examined. All the examined extracts showed moderate antioxidant capacity, whereas the fruit extracts were more active than the extracts of leaves. Also, the extracts of A. turbith subsp. hungarica exerted higher antioxidant capacity than corresponding A. turbith subsp. haynaldii extracts. The relationship between estimated activity and chemical composition of the extracts is discussed.

Chemical composition of Artemisia absinthium L. from Greece

Abstract The hydrodistilled essential oil of the aerial parts of Artemisia absinthium L. was analyzed by GC/MS. Sixty-eight components of the oil were identifed representing 93.4%. The main components were caryophyllene oxide (25.3%), p-cymene (16.8%), 1,8-cineole (8.9%) and (Z)-lanceol acetate (7.3%).

The Essential Oil of Valeriana officinalis L. s.l. Growing Wild in Western Serbia

Abstract The oil obtained by hydrodistillation from underground parts of Valeriana officinalis L. s.l., growing wild on Tara Mountain (western Serbia), was analyzed by means of GC/MS. Fifty-three components, representing 90.7% of the oil, were identified. The main characteristic of this valerian essential oil was the presence of valerianol (57.3%) and bornyl acetate (11.3%).

Comparative Analysis of Essential Oils of Six Anthemis Taxa from Serbia and Montenegro

The essential‐oil composition of six Anthemis taxa from several populations in Serbia and Montenegro, Anthemis triumfetti (L.) DC., A. tinctoria L., A. austriaca Jacq., A. ruthenica Bieb., A. cotula L., and A. cretica ssp. carpatica (Willd.) Grierson were analyzed by GC and GC/MS. The main constituents have been identified as follows: camphor (13.8–15.4%), α‐pinene (5.2–9.3%), β‐pinene (4.9–7.8%), and (E)‐caryophyllene (7.3–9.8%) in three populations, and cis‐chrysanthenol (27.0%) and 1,8‐cineole (8.4%) in one population of A. triumfetti; 1,8‐cineole (9.0–25.8%) in the oils of five populations, and borneol (16.0%) and spatulenol (16.0%) in the oil of one population of A. tinctoria; cis‐chrysanthenyl acetate (17.5–22.0%), β‐pinene (8.6–13.2%), and 1,8‐cineole (7.2–10.4%) in the oils of A. austriaca; germacrene D (8.3–11.3%) and terpinen‐4‐ol (6.3–7.3%) in A. ruthenica oils; β‐cedrene (10.3–19.0%), (E)‐β‐farnesene (7.8–13.5%), and germacrene D (5.2–9.1%) in the oils of A. cotula; cis‐thujone (39.0%), trans‐thujone (13.5%), and yomogi alcohol (7.1%) in the oil of A. cretica ssp. carpatica. The essential oil of A. austriaca was studied for the first time. A cluster analysis based on the relative percentages of all components of the essential oils was used to determine the distances between taxa and populations.

Composition and Antifungal Activity of the Oil from Aerial Parts and Rhizomes of Valeriana dioscoridis from Greece

Abstract The essential oils from aerial parts and rhizomes of Valeriana dioscoridis Sibth. et Sm. were investigated by GC/MS. The major components, among the identified 55, were patchouli alcohol (13.4% and 15.7%, respectively), α-pinene (6.7% and 8.6%, respectively), and β-pinene (5.8% and 7.0%, respectively). Furthermore, the antifungal activity of the oil from rhizomes and its main component patchouli alcohol was evaluated. The essential oil tested showed higher antifungal properties than patchouli alcohol. The commercial fungicide Bifonazole, which was used as a control, showed lower antifungal potential than the oil and patchouli alcohol investigated.

Chemical Composition of Pulicaria dysenterica (L.) Bernh. from Greece

Abstract The chemical composition of the aerial parts oil of Pulicaria dysenterica (L.) Bernh. was examined by GC and GC/MS. Oils hydrodistilled from samples collected from two different locations in Greece were found to possess minor compositional differences but considerable variation in the levels of some components. Fifty-four components were identified representing 80.5% (sample A) and 72.6% (sample B) of the total oils. The main components in sample A were (Z)-nerolidol (11.2%), caryophyllene oxide (9.1%) and (E)-nerolidol (6.6%), while those of sample B were β-caryophyllene (12.8%), caryophyllene oxide (12.8%) and (E)-nerolidol (6.9%).

ESSENTIAL OIL OF Filipendula hexapetala

The aerial parts of F. hexapetala yielded 0.1% (w/w) of yellowish oil. The results of GC and GC/MS analyses of the investigated oil are summarized in Table 1. Thirty-one compounds were identified, representing 90.8% of the total oil. The main constituent was identified as hydrocarbon n-tricosane (17.9%). The oil was characterized by a high content of salicylic acid derivatives: salicylaldehyde (13.7%), benzyl salicylate (6.8%), and methyl salicylate (6.7%). Besides, n-nonanal (11.9%), 2-heptadecanone (6.2%), and linalool (5.2%) were present in significant amounts, while other constituents were in less than 5%. The high content of salicylaldehyde (36.0%) and methyl salicylate (19.0%) was also found in the essential oil from

Composition and Antimicrobial Activity of Salvia amplexicaulis Lam. Essential Oil

Abstract The chemical analysis of the Salvia amplexicaulis Lam. herb essential oil was performed using GC-FID and GC/MS. Fifty-one compounds (90.2% of the total oil) were identified. The oil was characterized by a high amount of sesquiterpenes (81.1%), with germacrene D (14.8%), viridiflorol (10.6%), caryophyllene oxide (10.5%), and β-caryophyllene (9.4%) being the main components. The microbial growth inhibitory properties of the oil was determined against Gram-positive bacteria Staphylococcus aureus, St. epidermidis, Micrococcus luteus Gram-negative bacteria Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli and a yeast Candida albicans. The best inhibitory effect was detected against M. luteus followed by the effects against St. epidermidis, St. aureus and C. albicans. Tested Gram-negative bacteria were more resistant than Gram-positive bacteria and C. albicans.

Composition and Antimicrobial Activity of the Rhizome Essential Oils of Two Athamanta turbith Subspecies

Abstract The hydrodistilled essential oils of the rhizomes of Athamanta turbith subsp. hungarica and A. turbith subsp. haynaldii were analyzed by GC-FID and GC/MS. Twenty-one compounds (99.8% of total oil) were identified in the oil from A. turbith subsp. hungarica, and 33 components (99.9% of total oil) were identified in the oil from A. turbith subsp. haynaldii. Both oils were characterized by high amounts of phenylpropanes, with myristicin (54.2% and 22.3%, respectively) and apiole (32.6% and 48.5%, respectively) being the main components. The antimicrobial activity of the oils was determined using the agar diffusion and broth microdilution method. Investigated essential oils were the most effective against Micrococcus luteus and Klebsiella pneumoniae, and had no effect against Pseudomonas aeruginosa. As for antifungal activity, essential oils exerted similar and significant activity, but only in higher concentrations.