Miloslav Podojil

Determination of fatty acids in algae by capillary gas chromatography—mass spectrometry

Abstract Capillary gas chromatography and mass spectrometry were used to determine fatty acids in algae of the genera Chlorella and Scenedesmus , and in the blue-green alga Spirulina platensis . Most algae studied were cultivated autotrophically; Chlorella kessleri was also cultivated heterotrophically. In addition to commonly occurring fatty acids in green and blue-green algae, further acids, not previously described in these organisms, were detected. The novel features of these fatty acids were the branching position, cyclopropane ring, number and position of double bonds and chain length.

Gas chromatography—mass spectrometry and desorption chemical ionization mass spectrometry of triacylglycerols from the green alga chlorella kessleri

Abstract The distribution of triacyglycerols in the green alga Chlorella Kessleri cultivated heterotropically were determined by means of gas chromatography-mass spectrometry and desorption chemical ionization mass spectrometry. Quantitative and qualitative results obtained by the two methods are correlated.

The very long chain fatty acids of the green alga,Chlorella kessleri

Fatty acids of the fresh-water green alga,Chlorella kessleri (heterotrophically cultivated), were fractionated (as methyl esters) using high performance liquid chromatography (HPLC). A fraction of fatty acids with chain lengths longer than 28 carbon atoms was analyzed by gas chromatography-mass spectrometry (GC-MS). The very long chain fatty acids, ranging from 31 to 36 carbon atoms, were found for the first time in a green alga.

Identification of wax esters of the fresh-water green alga chlorella kessleri by gas chromatography-mass spectrometry

Abstract Wax esters were detected in the fresh-water green alga Chlorella kessleri cultivated heterotrophically and their individual molecular classes of up to 50 carbon atoms were identified by means of capillary gas chromatography-mass spectrometry. The wax esters are composed from very long-chain fatty acids up to dotriacontanoic acid and alcohols up to 13-eicosenol with hexadecanol as a major representative. Monoenic acids have an unusual position of the double bond, namely at the n-18 position.

TLC and HPLC of Mixture of Anthracyclinones

Abstract A combination of TLC and HPLC was used to separate a natural mixture of anthracyclinones /daunomycinone, 7-deoxydaunomycinone, 7-deoxy-13-dihydrodaunomycinone, 13-dihydrodaunomycinone, carminomycinone, 13-dihydrocarminomycinone, “bisanhydrocarminomycinone” and e-rhodomycinone/. The method is suitable for routine analysis of anthracyclinones in the course of their biosynthesis in production cultures and during in vitro biotransformations.

High-Performance Liquid Chromatography of New Semisynthetic Daunomycinone Derivatives

Abstract Reversed-phase HPLC utilizing LiChrosorb RP-8 was used to separate reaction mixtures of new semisynthetic daunomycinone derivatives and determine their relative occurrence. Chromatographic behaviour of the following compounds was studied: daunomycinone (I), 7(S) and 7(R)-0-(2-hydroxyethyl)-13-ethyleneacetal daunomycinone (II and III), 13-ethyleneacetal daunomycinone (IV), 13-ethyleneacetal bisanhydrodaunomycinone (V), 7(S) and 7(R)-0-(3-hydroxypropyl)-13-propyleneacetal daunomycinone (VI and VII), 13-propyleneacetal daunomycinone (VIII), 13-propyleneacetal bisanhydrodaunomycinone (IX), 7(S) and 7(R)-0-(4-hydroxybutyl) daunomycinone (X and XI), 4-toluenesulfonylhydrazone daunomycinone (XII).