Min Bu

A phase retrieval method of interferograms add-subtracting based on two-step phase shifting

A phase retrieval method is introduced in quantitative phase imaging (QPI) based on two-step phase-shifting technique. By acquiring two measured interferograms and calculating the addition and subtraction between them, the quantitative phase information can be directly retrieved. This method is illustrated by both theory and simulation experiments of a ball. The results of the simulation and the experiment of the red blood cell show a good agreement, demonstrating its application for studying cells.

Study of Phase Function of the Biological Cell

In order to settle the influence of measuring results of cells with its shape-variety in the examination of biological cell, MCEM (modify co-central ellipse model) is adopted based on the theories of Rayleigh-Debye-Gans, and the changing tendency of scattering phase function of nucleated cells with different body factor, nuclear size and shooting angle are studied. These provide a useful theory foundation for improving the measurement and distinguishing of biological cell.

Phase Microscopy Method of Micro-Nano Sized Bubbles Based on Hilbert Phase Microscopy (HPM)

Measuring shape of bubbles is very important in many industrial processes, because that its behavior in the fluid is closely related to its morphology. Phase microscopy imaging (PMI) method is one of the best useful methods in this field. In the paper, considering on PMI idea, it is put out a new method which improves an ordinary light microscope into a dual function that can do both PMI and its ordinary microscopy function. Its optical structure is designed by using Mach-Zehnder interferometer method which can be added on the platform of ordinary microscope. A glass hole (bubble) is used as a sample to do phase microscopy imaging via the improved device. The results of the experiment and theory show that the phase distribution of bubble is closely related to the shape of it, which is very useful to detect the bubble’s behavior in the flow field. Besides bubbles, the improved microscope can be also used to observe the phase body such as cells.

Light Scattering Images of Amplitude, Phase and Polarization from Typical Blood Cells

With the development of DPM (digital phase microscopy) and FCM (flow cytometry), techniques of cell recognition are getting more and more applications in many fields. Researches on the information from cells are very important to these techniques. In this paper, focusing on the technique of analyzing light scattering information and its relation with the morphological structures of the leucocytes and erythrocytes have been done. Based on the VirtualLab simulation techniques, the light scattering images of amplitude, phase and polarization of these typical cells have been obtained. It is found that the amplitude, phase and polarization of these cells are closely related to their morphological structures by analyzing the images, which is very useful to identify biological cells.

Research on the Phase Distribution of White Blood Cells with Nucleus Model under VirtualLab Technique

Focusing on the application technique of the phase testing techniques in cell identification, the analysis to the morphological structures of the leucocytes is done, and the physical models with nucleus of white blood cells are built. Based on the VirtualLab and numerical simulation techniques to systematic study on the distribution of the phase of the the leucocytes, the inside and outside morphological structures of the leucocytes and the main characteristics of the models are found. Thus, the phase testing application has been expanded. The light phase distribution analysis of biological cell is expected to become a new tool to detect the biological cell.

Temperature Distribution in Focused Area of a Cell by Short Pulse Laser

For studying the effect between short laser pulse and red blood cell (RBC) in a more accurate and powerful way, the light beam characters of a focused femtosecond pulse are studied with a simulation method, and then, a mixed wave-propagating model (MWP) is put forward after analyzing these characters, in which, light beam is thought as propagating in a Gaussian focused shape. In addition, a mathematical processing and linear absorption model in the focused irradiated region of RBC is built based on analyzing its physical mechanism. Finally, the temperature distribution within the RBC is gotten by data simulating calculation, which shows that the temperature variety caused by linear absorption in the focal region is rather small.

Mode Analysis of Several Typical Blood Cells under Optical Phase Models by VirtualLab Technique

In order to get more information and improve classification precision of the cell, the paper chooses several typical blood cells as main study objects, and sets up the related optical phase models. Then, according to the optical interference theory and related experimental parameters choosing, the VitualLab imitation system is applied to design relevant modules to get the phase distributions of these blood cells. The paper shows that different cells have different phase distributions after unwrapping of these phase distributions, which provides preferable theoretic basis and technical support for improving the inspecting precision of the blood cells.