Min-Hua Cui

Effect of electrode position on azo dye removal in an up-flow hybrid anaerobic digestion reactor with built-in bioelectrochemical system

In this study, two modes of hybrid anaerobic digestion (AD) bioreactor with built-in BESs (electrodes installed in liquid phase (R1) and sludge phase (R2)) were tested for identifying the effect of electrodes position on azo dye wastewater treatment. Alizarin yellow R (AYR) was used as a model dye. Decolorization efficiency of R1 was 90.41 ± 6.20% at influent loading rate of 800 g-AYR/ m3·d, which was 39% higher than that of R2. The contribution of bioelectrochemical reduction to AYR decolorization (16.23 ± 1.86% for R1 versus 22.24 ± 2.14% for R2) implied that although azo dye was mainly removed in sludge zone, BES further improved the effluent quality, especially for R1 where electrodes were installed in liquid phase. The microbial communities in the electrode biofilms (dominant by Enterobacter) and sludge (dominant by Enterococcus) were well distinguished in R1, but they were similar in R2. These results suggest that electrodes installed in liquid phase in the anaerobic hybrid system are more efficient than that in sludge phase for azo dye removal, which give great inspirations for the application of AD-BES hybrid process for various refractory wastewaters treatment.

Response of anodic bacterial community to the polarity inversion for chloramphenicol reduction

Chloramphenicol (CAP) is a frequently detected environmental pollutant. In this study, an electroactive biofilm for CAP reduction was established by initially in the anode and then inverting to the cathode. The established biocathode could enhance the reduction of CAP to the nitro-group reduced CAP (AMCl2) and further dechlorinated form (AMCl), both had lost the antibacterial activity. The phylogenetic diversity of the acclimated biofilm was decreased after the polar inversion. Proportions of functional bacterial genera, including Geobacter, Desulfovibrio and Pseudomonas responsible for the bidirectional electron transfer and nitroaromatics reduction, had increased 28%, 104% and 43% in the cathode. The relatively high abundances (over 50%) of Geobacter in anode and cathode were rarely detected for the nitroaromatics reduction. This study provides new insights into the electroactive biofilm structure improvement by the polarity inversion strategy for refractory antibiotics degradation.

Decolorization enhancement by optimizing azo dye loading rate in an anaerobic reactor

An up-flow anaerobic sludge blanket (UASB) reactor was developed to investigate the effect of azo dye loading rate on the decolorization performance and microbial community. The results indicated that the decolorization efficiency decreased from 95.84 ± 2.60 to 62.98 ± 8.66% with the azo dye loading rate increasing from 100 to 800 g m−3 d−1. The inhibition of the high azo dye loading rate on the microbial activity was reversible. The decolorization efficiency rose with the azo dye loading rate decreasing and recovered to 92.15 ± 3.86% at an azo dye loading rate of 600 g m−3 d−1. The results of 16S rRNA gene sequencing based on the high-throughput Illumina MiSeq sequencing showed that the microbial diversity of the sludge in UASB was reduced compared to the inoculum. Proteobacteria (40.51%) and Firmicutes (33.17%) were enriched. Enterobacter and Enterococcus with relative abundances of 26.99% and 20.38% were the most enriched genera, which were also functional for azo dye reduction.

Efficient azo dye decolorization in a continuous stirred tank reactor (CSTR) with built-in bioelectrochemical system.

A continuous stirred tank reactor with built-in bioelectrochemical system (CSTR-BES) was developed for azo dye Alizarin Yellow R (AYR) containing wastewater treatment. The decolorization efficiency (DE) of the CSTR-BES was 97.04±0.06% for 7h with sludge concentration of 3000mg/L and initial AYR concentration of 100mg/L, which was superior to that of the sole CSTR mode (open circuit: 54.87±4.34%) and the sole BES mode (without sludge addition: 91.37±0.44%). The effects of sludge concentration and sodium acetate (NaAc) concentration on azo dye decolorization were investigated. The highest DE of CSTR-BES for 4h was 87.66±2.93% with sludge concentration of 12,000mg/L, NaAc concentration of 2000mg/L and initial AYR concentration of 100mg/L. The results in this study indicated that CSTR-BES could be a practical strategy for upgrading conventional anaerobic facilities against refractory wastewater treatment.

Facile fabrication of carbon brush with reduced graphene oxide (rGO) for decreasing resistance and accelerating pollutants removal in bio-electrochemical systems

Low electrode resistance is crucial for achieving efficient reactions in bio-electrochemical system (BES), especially considering the factors of BES scaling-up and microbial effects. Graphene has revealed a cornucopia of potential applications due to its high conductivity and extraordinary electrochemical properties. Here, significant reduction of electrode resistance and increment of electrochemical activity were achieved by fabricating the three-dimensional carbon brush using reduced graphene oxide (rGO/carbon brush) through one-step electro-deposition without any binder. The rGO/carbon brush was employed as cathode in BES equipped with bio-anode for azo compound (AO7) removal. The charge transfer resistances of cathode part and whole cell were decreased by 89% and 65%, respectively. The reactor showed quickly start-up within 48 h with peak cycle current six fold increase relative to the control. AO7 decolorization efficiency reached 91.1 ± 0.1% at 4 h and 97.6 ± 0.4% at 6 h. Effective decolorization of AO7 was at rate up to 650.7 g AO7/m3·h. The results indicated that the advantages of graphene and three-dimensional carbon brush successfully improved the overall performance of BES and enhanced refractory pollutants removal when applied to specific wastewater.