Min Seo

PALEOPARASITOLOGICAL REPORT ON THE STOOL FROM A MEDIEVAL CHILD MUMMY IN YANGJU, KOREA

Previous studies have successfully shown evidence for parasitic infections in human remains from various archaeological sites. However, in the case of Korea, since there have been very few paleoparasitological reports published, pre-20th century parasitic infection patterns remain obscure. Therefore, in order to partly fill this gap, we are reporting on a case of paleoparasitic infection from the feces of a 15th century child mummy from Yangju, Korea. In the course of the present study, we found the eggs of Clonorchis sinensis, Ascaris lumbricoides, and Trichuris trichiura in the feces of the mummy. Trichuris trichiura eggs were found in far greater numbers than other parasite eggs; in fact, intact bipolar plugs were clearly observed and even the larvae were still visible in some eggs. The eggs of C. sinensis and A. lumbricoides were also well preserved, though not in as great a number. Since we could find a number of well-preserved larvae-containing eggs, we are encouraged that successful extraction, amplification, and sequence determination of ancient DNA from the paleoparasite eggs might be possible in future studies. With additional paleoparasitological investigation using feces from Korean mummies, we hope that a history of parasite infection in Korea will be reconstructed.

Gymnophalloides seoi Eggs from the Stool of a 17th Century Female Mummy Found in Hadong, Republic of Korea

It was previously reported that paleoparasitological clues for parasites infecting humans could be found in the feces of mummies of the Joseon Dynasty (1392–1910) in the Republic of Korea. Here, we report the presence of trematode eggs, including Clonorchis sinensis, Metagonimus yokogawai, and Gymnophalloides seoi (a human parasite known in Korea since 1993) in the feces of a recently excavated female mummy in Hadong, Republic of Korea. This is the first report of the discovery of a G. seoi infection in a human mummy. Since Hadong is currently not an endemic area for G. seoi, we speculate that the parasite might have occurred frequently along coastal areas of the Korean peninsula several hundred years ago and that the endemic areas contracted to, more or less, restricted regions since that time.

SCANNING ELECTRON MICROSCOPE STUDY OF ANCIENT PARASITE EGGS RECOVERED FROM KOREAN MUMMIES OF THE JOSEON DYNASTY

Abstract We have previously shown that parasite eggs have been identified in the coprolites of Korean mummies. These eggs have shed light on parasitic infection patterns in Korean populations living several hundred years ago. We conducted a scanning electron microscopy (SEM) study on ancient Trichuris trichiura, Ascaris lumbricoides, Metagonimus yokogawai, Paragonimus westermani, and Gymnophalloides seoi eggs recovered from Korean mummies of the Joseon Dynasty. We anticipated that the taphonomic conditions of mummification would alter the eggs of certain species but not of others. Our SEM data show that each species of ancient egg exhibited different degrees of preservation. Thus, some of them, for example, M. yokogawai, exhibited a better preservation status than others, suggesting that they should be the first candidates considered when choosing subjects for future paleoparasitological studies.

Susceptibility of Various Species of Animals and Strains of Mice to Gymnophalloides seoi Infection and the Effects of Immunosuppression in C3H/HeN Mice

Susceptibility to Gymnophalloides seoi infection was studied in 8 species of animals, including 7 strains of mice; the effects of immunosuppression on susceptibility were examined in C3H/HeN mice. One hundred metacercariae of G. seoi isolated from naturally infected oysters were orally administered to each animal. Worm recovery rate (WRR), worm dimensions, and the number of uterine eggs were obtained at day 3 and day 7 postinfection (PI). Average WRR from gerbils, hamsters, and cats at day 7 PI was 28.0%, 14.2%, and 10.9%, respectively, the former 2 figures of which were significantly higher than the rate of 0.0-4.0% from Sprague-Dawley rats, dogs, ducks, guinea pigs, and chicks. In the case of mice, average WRR at day 7 PI was 12.4% (KK strain), 11.8% (C3H/HeN), 9.6% (ICR), 6.4% (BALB/c), and 6.3% (ddY), respectively; the first 3 figures were significantly higher than the rates from other strains, which were 1.8% (A) and 0% (C57BL/6). At day 3 PI, WRR was much higher in all strains except C57BL/6. Worm maturation was the highest in C3H/HeN mice. Immunosuppression of C3H/HeN mice by injecting prednisolone for 7, 14, or 21 days prior to infection increased WRR at day 7 PI to 27.8%, 33.8%, or 67.5%, respectively. The results show that gerbils, hamsters, cats, and KK, C3H/HeN, ICR. BALB/c, and ddY mice are laboratory hosts that are fairly susceptible to G. seoi infection. In C3H/HeN mice, susceptibility was markedly enhanced by immunosuppression.

Prevalence of pediculosis capitis among Korean children

Pediculus humanus capitis is an ectoparasite, which causes scalp pruritus particularly among children. A total of 15,373 children including 8,018 boys and 7,355 girls from 26 primary schools and 15 kindergartens attached to the primary schools and a total of 33 children from an orphanage were examined for head lice infestation (HLI). The overall prevalence of HLI in this study was 4.1% including 3.7% of the urban areas and 4.7% of the rural areas. Head lice were found more frequently in girls than in boys with prevalence of 6.5% and 1.9%, respectively. The infestation rate by school grade was 3.2%, 4.7%, 4.2%, 5.0%, 4.9%, 3.8%, and 2.1% for kindergarten, first, second, third, fourth, fifth, and sixth grades, respectively. The infestation rate of the children in an orphanage was 66.7%. The prevalence of HLI has decreased especially in the rural areas. However, HLI is a still health problem of kindergarteners and primary schoolchildren in Korea.

Paleoparasitological report on Ascaris aDNA from an ancient East Asian sample.

In this study, Ascaris DNA was extracted and sequenced from a medieval archaeological sample in Korea. While Ascaris eggs were confirmed to be of human origin by archaeological evidence, it was not possible to pinpoint the exact species due to close genetic relationships among them. Despite this shortcoming, this is the first Ascaris ancient DNA (aDNA) report from a medieval Asian country and thus will expand the scope of Ascaris aDNA research.

The Influence of Differential Burial Preservation on the Recovery of Parasite Eggs in Soil Samples from Korean Medieval Tombs

Abstract The present study showed that ancient parasite eggs, not commonly present in soil samples from medieval Korean tombs, have been found in a very limited number of cases that satisfy certain archaeological requirements. In our paleo-parasitological examination of soil samples from medieval tombs encapsulated by a lime soil mixture barrier (LSMB), parasite eggs were more commonly detected in tombs that contained remains with clothes, hair, or brain tissue, though samples from not all such tombs contained eggs. Nonetheless, there was a close correlation between the preservation of certain types of cultural or human remains and the presence of ancient parasite eggs within medieval Korean LSMB tombs. Such remains, therefore, could be regarded as a strong predictor of well-preserved ancient parasite eggs in soil samples from LSMB tombs.

Paleoparasitological studies on mummies of the Joseon Dynasty, Korea.

Paleoparasitology is the application of conventional or molecular investigative techniques to archeological samples in order to reveal parasitic infection patterns among past populations. Although pioneering studies already have reported key paleoparasitological findings around the world, the same sorts of studies had not, until very recently, been conducted in sufficient numbers in Korea. Mummified remains of individuals dating to the Korean Joseon Dynasty actually have proved very meaningful to concerned researchers, owing particularly to their superb preservation status, which makes them ideal subjects for paleoparasitological studies. Over the past several years, our study series on Korean mummies has yielded very pertinent data on parasitic infection patterns prevailing among certain Joseon Dynasty populations. In this short review, we summarized the findings and achievements of our recent paleoparasitological examinations of Joseon mummies and discussed about the prospects for future research in this vein.

Finding Ancient Parasite Larvae in a Sample From a Male Living in Late 17th Century Korea

Abstract Parasitological examination of samples from tombs of the Korean Joseon Dynasty (1392–1910) could be helpful to researchers in understanding parasitic infection prevalence in pre-industrial Korean society. Whereas most of our previous parasitological studies revealed the presence of ancient parasite eggs in coprolites of Korean mummies, a sample from a man living in late 17th century Korea proved to be relatively unique in possessing what appeared to be several species of parasite larvae. The larvae identified included Strongyloides stercoralis and Trichostrongylus spp., along with eggs of Ascaris lumbricoides, Trichuris trichiura, and Paragonimus westermani. Since ancient parasite larvae retain enough morphology to make proper species identification possible, even after long burial times, the examination of parasite larvae within ancient samples will be conducted more carefully in our future work.

A Seroepidemiological Survey of Toxocariasis among Eosinophilia Patients in Chungcheongnam-do

There have been several reports on the relationship between toxocariasis and eosinophilia, but all have been limited to the areas of Seoul or Gangwon-do. In the present study, we investigated the seroprevalence of toxocariasis among eosinophilia patients in Chungcheongnam-do, the central district of Korea. Among the 101 patients tested, 51 (50.5%) were identified as positive by Toxocara ELISA, and 46 (45.5%) were confidently diagnosed with toxocariasis because of absence of any other cause of eosinophilia. Whereas 22 of 42 seropositive patients (52.3%) had a recent history of consuming raw livers, especially the cow liver, only 1 of 25 seronegative patients (4%) had done so (P<0.01). From these results, we could confirm that toxocariasis is related to eosinophilia, and infer that ingestion of raw cow liver plays a vital role in the transmission of toxocariasis in Chungcheongnam-do.