Mineo Matsubara
Kansai Medical University
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Featured researches published by Mineo Matsubara.
Archives of Biochemistry and Biophysics | 1991
Yasuhide Kitazawa; Mineo Matsubara; Naoshi Takeyama; Takaya Tanaka
The role of xanthine oxidase in the mechanism of paraquat toxicity was assessed by in vitro and in vivo experiments. Paraquat stimulated the reduction of cytochrome c by xanthine-xanthine oxidase system in vitro. Paraquat, when added in vitro, stimulated hypoxanthine-dependent superoxide production in the cytosol of rat lung. Tungsten-feeding inhibits xanthine oxidase activity in a variety of tissues in experimental animals. Its therapeutic effect on paraquat intoxication was studied in this paper. In rats fed a tungsten-enriched diet for 5 weeks prior to intraperitoneal injection of 50 mg/kg paraquat dichloride, the mortality decreased significantly compared with rats fed a standard diet. Pretreatment with oxypurinol (1000 mg/kg, s.c.) also ameliorated the paraquat toxicity in rats. We conclude that xanthine oxidase plays an important role in paraquat toxicity and that xanthine oxidase inhibitors may become antidotes for paraquat intoxication.
Archives of Toxicology | 1996
Mineo Matsubara; Kazunobu Yamagami; Yasuhide Kitazawa; Keiji Kawamoto; Takaya Tanaka
Abstract We examined the in vivo effect of paraquat on the cell cycle in rat liver and lung tissues and the protective effect of tungsten (a xanthine oxidase inhibitor) on paraquat toxicity. The bromodeoxy- uridine/propidium iodide double-staining method and flow cytometry were used for cell cycle assessment. Wistar rats were fed a standard diet or a tungsten-enriched diet were injected intravenously with 20 mg/kg paraquat, while uninjected rats served as controls. At 1, 3, and 5 days after paraquat injection, the liver and lungs were removed for examination following in vivo labeling with 20 mg/kg bromo- deoxyuridine for 1 h. Liver and lung cells were isolated and incubated with an anti-bromodeoxyuridine antibody and with propidium iodide for DNA staining. Flow cytometry showed that the S-phase cell populations in the liver and lungs of paraquat-injected rats fed a standard diet were increased markedly on days 1 and 3 after injection compared with the control levels. However, on day 5 the liver cells had nearly returned to normal, while the S-phase population remained high in the lungs. In contrast, the S-phase cell populations of liver and lung tissue showed no increase after paraquat injection in rats fed a tungsten-enriched diet. These findings suggest that paraquat-induced cytotoxicity is more prolonged in the lungs than in the liver. In addition, paraquat toxicity appears to be mediated by xanthine oxidase and xanthine oxidase inhibitors may be useful as an antidote.
Journal of Analytical Toxicology | 1996
Hiroyasu Ishikura; Nobuaki Matsuo; Mineo Matsubara; Takashi Ishihara; Naoshi Takeyama; Takaya Tanaka
Digestion | 1991
Yoshitoshi Itoh; Kenji Suzuki; Mineo Matsubara; Naoshi Takeyama; Yasuhide Kitazawa; Takaya Tanaka
Journal of Applied Toxicology | 1994
Kazunobu Yamagami; Mineo Matsubara; Yasuhide Kitazawa; Naoshi Takeyama; Takaya Tanaka; Keiji Kawamoto
The journal of Kansai Medical University | 1990
Takaya Tanaka; Naoshi Takeyama; Mineo Matsubara; Hideharu Yamanaka; Koshiro Hioki; Masakatsu Yamamoto
熱傷 | 1997
Yasuhide Kitazawa; Masaya Kiritohshi; Mineo Matsubara; Nobuaki Matsuo; Takaya Tanaka; Hideaki Doi; Shigeo Kyutoku; Yutaka Ogawa
Nihon Kyukyu Igakukai Zasshi | 1996
Michiya Akahori; Hiroyasu Ishikura; Nobuaki Matsuo; Mineo Matsubara; Yasuhide Kitazawa; Takaya Tanaka
Nihon Kyukyu Igakukai Zasshi | 1996
Yutaka Okii; Atsushi Akane; Keiji Kawamoto; Mineo Matsubara; Kazutada Yamagami; Takaya Tanaka; Takao Chishiro
Nihon Rinsho Geka Gakkai Zasshi (journal of Japan Surgical Association) | 1993
Yoshikazu Shoji; Megumi Sakai; Mineo Matsubara; Tetsuya Moriguchi; Masahiko Hikima; Takaya Tanaka