Minerva Maldonado-García

Evaluation of ToxA and Vibrio parahaemolyticus lysate on humoral immune response and immune-related genes in Pacific red snapper.

Immunogenicity of ToxA and Vibrio parahaemolyticus lysate was evaluated in a double immunostimulation scheme in Pacific red snapper after V. parahaemolyticus infection. Three groups of Pacific red snapper were intraperitonealy (i.p.) injected with phosphate-buffered saline (PBS group), ToxA of V. parahaemolyticus (ToxA-Vp group) or V. parahaemolyticus lysate (lysate-Vp group) (first injection, day 1; second injection, day 7). Fish were subsequently infected with live V. parahaemolyticus. Humoral immune parameters in skin mucus and serum were evaluated on days 1, 7, 8 and 14 days post-immunostimulation and 7 days post-infection. Moreover expression of immune-related genes was quantified by real time PCR in head-kidney leukocytes, spleen, liver, and intestine. The ToxA-Vp-treated group showed a higher anti-protease and catalase activity in skin mucus when compared with the PBS group. Measurements of SOD and CAT activities showed an increment in both activities a day after the second boost with ToxA-Vp or lysate-Vp. Interestingly, IgM levels in mucus and transcripts were enhanced followed the ToxA-Vp treatment even after challenge. Furthermore, IL-1β was strongly expressed in all analyzed cell or tissues followed ToxA-Vp or Vp-lysate treatments. Finally, SOD and CAT gene expression was up-regulated in fish immunostimulated with either treatment ToxA-Vp or lysate-Vp, mainly after infection in head-kidney leukocytes and intestine. This is the first study where the effects of ToxA from V. parahaemolyticus in the immune system of Pacific red snapper was evaluated. These results suggest that ToxA-Vp would positively affect humoral immune response and up-regulate expression of genes involved in the immune system function; and could help in the control of V. parahaemolyticus infection in Pacific red snapper Lutjanus peru, an economic important fish in Mexico.

Feeding habits of Mustelus henlei on the western coast of Baja California Sur, México

Palabras clave: Contenido estomacal, superposicion de dieta, especialista, Punta Lobos, Baja California Sur, Mexico Abstract.- The Brown smooth-hound Mustelus henlei is part of an important commercial fishery on the western coast of Baja California Sur (BCS), Mexico. However, very few studies have been performed on the feeding and reproduction biology of these sharks in Mexico. Our study is the first work focusing on this species on the western shelf (14 m to 250 m in depth) of B.C.S. We identified a total of 24 types of prey contents in the stomachs of M. henlei: 15 crustaceans, 6 fish, and 3 cephalopods. According to the index of relative importance (IRI) the main prey items for M. henlei was the pelagic red crab Pleuroncodes planipes (81.4%), followed by unidentified organic material (UOM) (15.7%), the mackerel Scomber japonicus (0.94%), and fish remains (0.84%). Conducting a prey analysis considering sex of M. henlei, P. planipes was the most important prey in both females and males, followed by UOM, and S. japonicus. Sharks found in deeper areas (120-150 m) and those caught on board the exploration ship BIP XII had a similar dietary composition to those found in the shallow waters of Punta Lobos (14-40 m) where for both regions, the dominant prey items were the pelagic red crab and UOM. According to Levins index, M. henlei may be a specialist feeder in this area. A diet overlap according to sex, area, and size was observed. The SIMPER analysis showed a low similarity in diet in relation to sex, size, and area. According to the ANOSIM test there were no significant differences. Knowing that M. henlei shows a specialist behavior could help research studies not only for species conservation but also for its habitat and the organisms that inhabit it, which are both of vital importance for this shark.

First record of spine malformation of the round stingray Urobatis halleri off the Western Coast of Baja California Sur, México

BackgroundMalformations of the Round stingray Urobatis hallerii, Cooper 1863 captured off the southwestern coast of Baja California Sur, México are reported in this study.MethodsThe ray was an adult male captured by artisanal fishery in the area of Estero Banderitas. Size measurements of total length and disk width were taken; the organism could be photographed when captured.ResultsMalformations of U. hallerii were found in the posterior part of the spine, a distortion in the upper part of the tail and in the left fin close to the tail. The specimen was considered able to reach the adult size usually reported in other specimens of this species despite the spine malformations because of its benthic habits and not losing its mobility completely.ConclusionsThe observed malformations were likely due to genetic alterations although other studies are needed to see if other environmental factors could cause malformations in marine organisms mainly in fish and elasmobranchs.