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Dive into the research topics where Minoru Sawano is active.

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Featured researches published by Minoru Sawano.


Scientia Horticulturae | 1984

Effects of bud scales and gibberellins on dormancy of in vitro cultured Japanese pear leaf buds

Takao Ichii; Minoru Sawano; Tetsu Nakanishi; Takeshi Ozaki

Abstract Leaf buds of Japanese pear were collected in early June and early November and regarded as summer and winter dormant buds, respectively. Bud explants with and without scales were prepared from each of them, and cultured in vitro for 75 days at 25°C with 14 h photoperiod, on a medium either without growth regulators, or supplied with BA and GAs (GA 3 and GA 4+7 ), singly or in combination. When either BA or GA 4+7 was contained in the medium, bud expansion occurred. Thereafter, summer dormant buds grew into shoots in the presence of BA, while winter dormant buds, although they swelled profusely, remained in a rosette. In the presence of BA, GA 4+7 markedly stimulated shoot elongation of summer dormant buds, but GA 3 did not. In winter dormant buds, GA 4+7 not only failed to stimulate shoot elongation, but also interfered with the BA-induced swelling described above. The presence of bud scales delayed expansion of summer dormant buds, while it had little effect on winter dormant buds. The delaying effect of scales on expansion of summer buds was effectively removed by application of GA 4+7 to the medium.


Journal of Chromatography A | 1984

Determination of indole-3-acetic acid and abscisic acid in Japanese chestnuts by column chromatography on sephadex G-10 and reversed-phase high-performance liquid chromatography

Nobuyuki Kozukue; Etsuko Kozukue; Lung-Ming Tsay; Minoru Sawano; Susumu Mizuno

Abstract A method for the separation and quantification of indole-3-acetic acid and abscisic acid in Japanese chestnuts has been developed by use of Sephadex G-10 column chromatography and a reversed-phase high-performance liquid chromatography (HPLC). For the first purification step, the plant hormones are extracted with methanol—water (80:20) then eluted as individual hormone fractions from a Sephadex column. The final purification was performed on a reverse phase (LiChrosorb RP-18, 5 μm), eluted with a linear gradient of 10–50% acetonitrile in 1% glacial acetic acid. The column effluent is monitored at 254 nm. This technique has been used effectively to analyze plant hormones in biological samples.


Breeding Science | 1995

High Frequency Regeneration of Diploids from Apical End of Cultured Hypocotyl Tissue in Tomato

Nobuaki Asakura; Shuji Misoo; Osamu Kamijima; Minoru Sawano


神戸大学農学部研究報告 | 1984

Studies on Identification of Chestnut Species and Varieties by Isozyme Analysis

Minoru Sawano; Takao Ichii; Tetsu Nakanishi; Zenko Kotera


Plant Biotechnology | 1991

Efficient Induction of Diploidized Plants in Anther Culture of Rice by Coichicine Pretreatment of Cold-Preserved Spikes

Shuji Misoo; Taihei Hirabayashi; Osamu Kamijima; Minoru Sawano


Plant Biotechnology | 1990

Effects of Plant Growth Regulators on the Response in Immature Wheat Embryo Culture

Hong-jun Liu; Shuji Misoo; Osamu Kamijima; Minoru Sawano


神戸大学農学部研究報告 | 1989

Effect of Genotype on the Response in Immature Embryo Culture of Japanese and Chinese Wheat

Shuji Misoo; Osamu Kamijima; Minoru Sawano


Breeding Science | 1992

Effects of Casein Hydrolysate on the Response in Immature Embryo Culture of Wheat:Triticum aestivum L

Hong-jun LlU; Shuji Misoo; Osamu Kamijima; Minoru Sawano


Agricultural and biological chemistry | 1991

Comparative Biochemistry of Serine Proteinase Inhibitors of Five Tuberous Solanum Species

Akihiro Shinzaki; Yasuo Aizono; Hiroshi Yamagata; Teruo Iwasaki; Minoru Sawano


Journal of The Japanese Society for Horticultural Science | 1984

Action of the Ripening Inhibitor (rin) on Fruit Ripening of Tomato

Minoru Sawano; Susumu Mizuno; Xu-Mang Sun; Nobuyuki Kozukue

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