Miodrag Palfi

A randomized controlled trial oftransfusion-related acute lung injury: is plasma from multiparous blood donors dangerous?

BACKGROUND: Transfusion‐related acute lung injury (TRALI) and other posttransfusion reactions may be caused by granulocyte and/or HLA antibodies, which are often present in blood from multiparous donors. The purpose of this study was to compare the effects of plasma from multiparous donors with those of plasma from donors with no history of transfusion or pregnancy (control plasma) in a prospective, randomized, double‐blind, crossover study.

Placental transport of maternal immunoglobulin G

PROBLEM: How does the placental transport of immunoglobulin G (IgG) vary with gestational age?

Measures to prevent transfusion-related acute lung injury (TRALI)

H. W. Reesink, J. Lee, A. Keller, P. Dennington, J. Pink, R. Holdsworth, H. Schennach, M. Goldman, T. Petraszko, J. Sun, Y. Meng, K. Qian, V. Rehacek, P. Turek, T. Krusius, E. Juvonen, P. Tiberghien, D. Legrand, G. Semana, J. Y. Muller, J. Bux, A. Reil, C. K. Lin, H. Daly, E. McSweeney, L. Porretti, N. Greppi, P. Rebulla, H. Okazaki, S. A. Sanchez-Guerrero, H. A. Baptista-Gonzalez, C. Martinez-Murillo, A. Guerra-Marquez, H. Rodriguez-Moyado, R. A. Middelburg, J. C. Wiersum-Osselton, A. Brand, C. van Tilburg, D. Dinesh, J. Dagger, P. Dunn, E. Brojer, M. Letowska, K. Maslanka, E. Lachert, M. Uhrynowska, E. Zhiburt, M. Palfi, G. Berlin, B. M. Frey, L. Puig Rovira, E. Muniz-Diaz, E. Castro, C. Chapman, A. Green, E. Massey, N. Win, L. Williamson, C. C. Silliman, D. J. Chaffin, D. R. Ambruso, N. Blumberg, P. Tomasulo, K. J. Land, P. J. Norris, O. C. Illoh, R. J. Davey, R. J. Benjamin, A. F. Eder, L. McLaughlin, S. Kleinman & S. Panzer

D variants at the RhD vestibule in the weak D type 4 and Eurasian D clusters

BACKGROUND: One branch of the RHD phylogenetic tree is represented by the weak D type 4 cluster of alleles with F223V as the primordial amino acid substitution. F223V as well as a large number of further substitutions causing D variants are located at the extracellular RhD protein vestibule, which represents the entrance to the transmembraneous channel of the RhD protein.

Circulating Interferon‐γ‐ and Interleukin‐4‐Secreting Cells in Recurrent Spontaneous Abortions

PROBLEM: Are recurrent spontaneous abortions (RSAs) associated with deviation of circulating cytokine‐secreting cells?

Blocking Antibodies in Blood from Patients with Recurrent Spontaneous Abortion in Relation to Pregnancy Outcome and Intravenous Immunoglobulin Treatment

PROBLEM: To study whether the occurrence of mixed lymphocyte culture (MLC) blocking antibodies is associated with pregnancy outcome in women with unexplained recurrent spontaneous abortion (RSA) and the in vivo effect of intravenous immunoglobulin (IVIG) treatment on MLC blocking effect.
 METHOD OF STUDY: Blood samples from 41 RSA patients were obtained before and after pregnancy, and blocking antibodies were estimated by one‐way MLC assay. The patients received IVIG or placebo (saline) during pregnancy. Additionally, pre‐pregnancy blood samples from 31 RSA women and 10 controls were obtained.
 RESULTS: We found no correlation between blocking antibodies before pregnancy and the pregnancy outcome. The occurrence of blocking antibodies was not affected by pregnancy or IVIG treatment.
 CONCLUSIONS: Blocking antibodies have no predictive value for the pregnancy outcome in RSA patients, and their production seems not to be affected by IVIG.

T and B lymphocyte subsets in patients with unexplained recurrent spontaneous abortion : IVIG versus placebo treatment

Jablonowska B, Palfi M, Matthiesen L, Selbing A, Kjellberg S, Ernerudh J. T and B Lymphocyte subsets in patients with unexplained recurrent spontaneous abortion: IVIG versus placebo treatment. AJRI 2002; 48:312–318

Placental Transport of Maternal Immunoglobulin G in Pregnancies at Risk of Rh (D) Hemolytic Disease of the Newborn

PROBLEM: The following questions were addressed: Is the placental transport of immunoglobulin (Ig)G, IgG1, and IgG3 diminished in pregnancies at risk of hemolytic disease of the newborn? Is the placental transport of IgG, IgG1, and IgG3 correlated with the hemoglobin concentration in the fetus and AutoAnalyzer (AA) quantitations of maternal anti‐D?

Application of the gel test in IgG subclassing--a comparison of two agglutination assays.

Background and objectives: IgG subclasses of anti‐D seem to play some role in hemolytic disease of the newborn, but there has been disagreement as to its exact nature. The aim of our study was to evaluate a new technique for IgG subclassing of anti‐D and to compare it to an established test. Materials and methods: In 31 cases of RhD immunization, we have compared two simple agglutinating assays for subclassing anti‐D: an established V‐well microtiter tray assay and a new gel test assay. Polyclonal rabbit anti‐IgG agglutinating antibodies were tested against sensitized D‐positive red blood cells by both assays. Results: The anti‐D concentration in tested sera varied between 0.1 and 81 μg/ml (0.5–405 IU/ml). Both assays were simple to perform and the results correlated well. Conclusions: The gel test had two advantages: quick performance and easy interpretation.