Mirco Dalla Bona

Sublethal effects of trimethoprim on four freshwater organisms.

Sublethal effects of trimethoprim (TMP) were evaluated in four freshwater organisms: Pseudokirchneriella subcapitata and Lemna minor (growth inhibition), Daphnia magna (reproduction and growth inhibition) and Poecilia reticulata (swimming activity inhibition). Cytochrome P4501A induction was also evaluated in P. reticulata. TMP showed varying levels of toxicity in the four test performed, with NOEC for the various endpoints in the range of 3.12-25 mg L(-1). The compound was active on P. reticulata at concentration ≥ 50 mg L(-1) causing inhibition of swimming activity. In the same organism an induction of CYP1A protein, mainly in kidney, gills and intestine, was also detected. L. minor was more sensitive than unicellular algae to TMP, with a NOEC of 12.5 mg L(-1). The lowest NOEC (3.12 mg L(-1)) was obtained in D. magna reproduction test and then a Risk Quotient of <0.03 was calculated by comparing the PNEC (31.2 μg L) and the TMP concentrations usually detected in freshwater (<1 μg L(-1)). However, based on recently reported data, it was concluded that while TMP concentrations normally detected in surface water are below those able to evoke appreciable biological effects in the various aquatic organisms, TMP concentrations in aquaculture and hospital effluents can be one to three orders of magnitude higher. Furthermore, the co-occurrence and additive effects of other antifolic agents should be taken into account for a cautious risk assessment of the drug.

The sensitivity of Daphnia magna and Daphnia curvirostris to 10 veterinary antibacterials and to some of their binary mixtures

Aim of this study was to evaluate the suitability of Daphnia curvirostris for the acute toxicity test usually performed on Daphnia magna, and to compare the sensitivity of the two species toward 10 antibacterials [enrofloxacin (EFX), ciprofloxacin(CPX), sulfaguanidine (SGD), sulfadiazine (SDZ), sulfamethazine (SMZ), sulfaquinoxaline (SQO), sulfaclozine (SCZ), sulfamerazine (SMA), sulfadimethoxine (SDM) and trimethoprim (TMP)] and some of their binary mixtures. Furthermore, a tentative prolonged-toxicity test (lasting 13d) was settled up in order to evidence toxic responses with drug concentrations that were uneffective in the classic 48h immobilization test. Results showed that D. curvirostris was more sensitive than D. magna to the majority of compounds (6 out of 10). Lowest 48h EC50s were obtained with EFX (4.3mgL(-1) in D. curvirostris) and SGD (6.2mgL(-1) in D. magna). The toxicity of paired compounds was always concentration-additive or less than concentration-additive. In the prolonged-toxicity test mortality and/or reproduction inhibition were constantly observed. It was concluded that: (1) D. curvirostris could be a suitable model for the evaluation of acute toxicity of antibacterials since its sensitivity was generally greater than that of D. magna; (2) the toxicity of EFX and SGD should be given special attention as the two compounds, in the prolonged test, showed to be active at concentrations of 0.9mgL(-1) and 2.5mgL(-1), respectively; (3) the concentration addition is usually a reasonable worst case estimation of the environmental impact of antibacterial mixtures.

Effects of enrofloxacin, ciprofloxacin, and trimethoprim on two generations of Daphnia magna.

Multigenerational tests on Daphnia magna were performed exposing two subsequent generation to enrofloxacin (EFX) and its metabolite ciprofloxacin (CPX), and to trimethoprim (TMP). Mortality rate of 100% and 50% was detected in F0 at concentrations of ≥ 13 mgL(-1) (EFX) and 50 mgL(-1) (TMP), respectively. In F1 with respect to F0, both for growth and reproduction, a worsening trend of the response with EFX, a similar response with CPX and an attenuating trend with TMP was observed. Furthermore, the lowest EC20 for reproduction inhibition (1.3 mgL(-1)) was calculated for F1 exposed to EFX. However, other experimentations, longer and more complex, are necessary in order to confirm that EFX is more hazardous to daphnids than CPX and TMP. EC50 measured for the three assayed antibacterials were in the 6.5-37 mgL(-1) range therefore environmental unrealistic, except in case of exceptional contaminations that may occur in relation to poorly controlled wastewaters from pharmaceutical factories or excessive use of prophylactic treatments in aquaculture.

Effect of progressive reduction in crude protein and lysine of heavy pigs diets on some technological properties of green hams destined for PDO dry-cured ham production

In order to investigate the effects of dietary crude protein (CP) and lysine (Lys) content on some technological properties of green hams destined for Protected Designation of Origin (PDO) dry-cured ham, green hams visual appraisal scores, thickness, iodine number and fatty acid composition of subcutaneous fat, and ham weight losses during seasoning (SL) were assessed. The green hams were obtained from 233 pigs fed four diets containing 140 to 110g/kg CP and 6.5 to 5.3g/kg total Lys from 90 to 165kg body weight. A reduction in dietary CP and Lys of up to 20% compared with conventional feeds led to a 15% increase in the thickness of the subcutaneous fat, a 5% decrease in linoleic and polyunsaturated fatty acids in subcutaneous fat and a 7% decrease in SL. A 20% reduction of CP and Lys in diets for finishing pigs has positive effects on the technological properties of green hams destined for PDO dry-cured ham production.

Growth performance, carcass traits and meat quality of growing pigs on different feeding regimes slaughtered at 145 kg BW

Abstract This study investigated the effects of feeding regime on growth performance, carcass traits and meat quality of pigs slaughtered at around 145 kg BW. A total of 96 barrows housed in eight pens were allotted to three groups in each pen. One group was fed ad libitum (AL) and the others were fed according to two quasi AL feeding regimes adjusting feed allowances with increasing BW. At slaughterhouse, the weights of the main lean and fat cuts were recorded, and a sample of longissimus lumborum (LL) was taken for physical and chemical analyses. Average daily gain (ADG) approached 940 g d−1, and gain to feed ratio (G:F) was close to 0.38. Compared with the AL-feeding regime, the feed restriction reduced the pigs’ ADG (−3.5%), feed intake (−7.4%) and carcass weight (−3%) (p < 0.01), but improved their G:F (+ 4%, p < 0.01). Feeding regime did not affect meat quality traits and exerted only minor effects on the weight of primal cuts and on the fatty acid composition of the intramuscular fat of the LL. However, AL-fed pigs tended to yield heavier fat cuts and showed a greater proportion of saturated fatty acid in the LL when compared to restricted feed barrows. In conclusion, moderate restriction in the feeding of medium–heavy pigs seems advisable, as it improves feed efficiency and could cut feed costs compared with the AL-feeding regime without affecting carcass and meat characteristics.

The influence of feeding behaviour on growth performance, carcass and meat characteristics of growing pigs

This study investigated the effect of the feeding behaviour on growth performance, and carcass and meat characteristics of 96 barrows fed ad libitum or restrictively with high or low amino acids (AA) diets according to a 2 × 2 factorial design. The feeding behaviour traits were measured with automated feeders. From 86 kg BW, half of the pigs were given feeds with high indispensable (AA) contents, while the other half received feeds with indispensable AA contents reduced by 9% in early finishing (86–118 kg BW) and by 18% in late finishing (118–145 kg BW). Body lipid and protein retentions were estimated from BW and backfat depth measures recorded at the beginning and end of each period. Pigs were slaughtered at 145 kg BW and carcass and meat quality data were recorded. Phenotypic correlations among feeding behaviours, growth performances, and carcass and meat traits were computed from all the data after adjustment for the effects of feeding treatments. As feeding rate was the behavioural trait most highly correlated with performance and carcass traits, the records of each pig were classified into feeding rate tertiles. Then, the data were statistically analysed using a mixed model, which included feed restriction (FR), AA reduction (AAR), the FR × AAR interaction and the feeding rate tertile as fixed factors, and pen as a random factor. Pigs eating faster (52.1 to 118.9 g/min) had significantly greater final body weights (16%), average daily weight gains (27%), estimated protein gains (22%), estimated lipid retention (46%), carcass weights (16%), weights of lean cuts (14%), weights of fat cuts (21%), proportions of fat in the carcass (14%), and 4% lower proportions of carcass lean cuts than pigs eating slowly (12.6 to 38.2 g/min). Manipulating the eating rate, through management or genetic strategies, could affect feed intake and subsequent growth performance, hence carcass quality, but have little influence on feed efficiency.

Effects of feed allowance and indispensable amino acid reduction on feed intake, growth performance and carcass characteristics of growing pigs

The hypothesis that pigs placed on diets with reduced indispensable amino acid (AA) content attempts to offset the reduction in the nutrient density with increased feed intake was tested. In the experiment, feeds with a high or a low AA content were administrated to pigs fed ad-libitum or restrictively according to a 2 × 2 factorial design. Ninety-six barrows were housed in 8 pens (12 pigs/pen) equipped with automatic feeders. Within pen, and from 47 body weight (BW) onwards, 6 pigs were fed ad libitum. The others pigs were allowed to consume, as a maximum, the feed amounts indicated by the breeding company feeding plane to optimize the feed efficiency. In early (86–118 kg BW) and late (118–145 kg BW) finishing, the pigs of 4 pens received feeds with high indispensable AA contents (8.1 and 7.5 g lysine/kg in the two periods, respectively). The other pigs received feeds with reduced indispensable AA contents (lysine, methionine, threonine and tryptophan) by 9 and 18% in early and late finishing, respectively. Body lipid and protein (Pr) retentions were estimated from BW and back-fat depth measures recorded at the beginning and the end of each period. Nitrogen excretion was estimated as actual intake minus estimated N-retention (Pr/6.25). Pigs were slaughtered at 144 kg BW. Restricted feeding decreased feed intake (-7%), daily gain (-5%), carcass weight (-2.6%) and back-fat depth (-8.0%) but increased gain:feed ratio (+2%). The AA restriction increased feed intake (+5.9%), carcass weight (+4.9%) and intramuscular fat (+17.6%), and reduced carcass weight variation (-36%), with no effects on the feed efficiency and the estimated Pr (142 g/d). N excreted was reduced by feed (-9%) and dietary AA (-15%) restrictions. Irrespectively of the feeding level, the pigs responded to a reduction of the dietary essential AA content by increasing their feed intake.

Effect of growth rate on live performance, carcass and green thigh traits of finishing Italian heavy pigs

Abstract This study aimed at investigating the effects of growth rate (GR) on live performance, carcass and dressed green ham (DRHAM) traits of finishing heavy pigs managed in accordance with the regulations governing the production of Protected Denomination of Origin (PDO) dry-cured hams. Data were obtained from 233 crossbred pigs restrictively fed from 90 to 165 kg BW. Individual BW, daily feed intake, and weights of carcass and main cuts were recorded. Thighs were measured for subcutaneous fat thickness, analysed for fatty acid composition of subcutaneous fat, and dry-cured hams were weighed. Records were classified according to GR quartile, calculated using the residuals of a statistical model that included the effects of pen, sex and diet. Data were analysed using the same model with the addition of the effect of GR quartile. Average GR ranged from 578 in the first quartile to 748 g/d in the fourth. Final BW and weights of carcass, DRHAM and dry-cured ham increased as the GR quartile increased (p < .01). An increase in GR improved feed efficiency linearly (p < .001) and tended to increase the thickness of subcutaneous fat of DRHAM, whereas seasoning losses decreased linearly with increasing average GR. In conclusion, targeting the GR of finishing pigs at a minimum of 750 g/d and consequently increasing final BW to around 175–180 kg may be a way to improve feed efficiency and some quality traits of DRHAM.