Miriama Kopernická

Contamination of wild-grown edible mushrooms by heavy metals in a former mercury-mining area

The aim of this study was to evaluate the contamination of six edible wild species of mushrooms (Boletus pulverulentus, Cantharellus cibarius, Lactarius quietus, Macrolepiota procera, Russula xerampelina and Suillus grevillei) by heavy metals (Hg, Cd, Pb, Zn, Cu, Ni, Cr, Co, Mn and Fe). Mushroom samples were collected from sites contaminated by emissions from mining and processing of polymetallic ores in operation during the period 1969–1993 in Rudňany, southeast Slovakia. The four study sites spanned up to a 5-km distance from the emission source. The collected mushroom samples were analyzed using Flame Atomic Absorption Spectrophotometry and/or Flame Atomic Absorption Spectrophotometry with graphite furnace. Mercury, Cd and, in some samples, also Pb present the highest risks in terms of contamination of the food chain following subsequent consumption. The content of two metals in the dry matter (dm) of the mushrooms exceeded the limits set by the European Union (EU; Cd: 0.5 mg/kg dm, Pb: 1.0 mg/kg dm). The highest mean contents of the eight metals recorded for S. grevillei were 52.2, 2.15, 107, 104, 2.27, 2.49, 81.6 and 434 mg/kg dm for Hg, Pb, Zn, Cu, Ni, Cr, Mn and Fe, respectively. The highest content of Cd was recorded in M. procera (3.05 mg/kg dm) and that of Co in L. quietus (0.90 mg/kg dm). The calculated weekly intake for Hg, Pb and Cd shows that regular consumption of mushrooms from the studied area poses risks to human health.

Copper content in cereals grown in the model condition

The consumption of cereals in Slovakia but also worldwide is increasing by every year. From 30000 to 50000 tons of mercury circulates thought the biosphere that gets into the atmosphere degassing of the earths crust and world oceans. Trace element as copper is one of the most efficient antioxidants in the body, often referred to as an element of beauty. It acts as a powerful catalyst for many enzymes and vitamins, through which already small amounts affects many activities in the body (strengthens immunity, reduces levels of histamine). It accumulates in the barley, beans, cucumber, nuts or milk and so on. The aim of our work was the evaluation of transfer of mercury from sludge to edible part of chosen cereals. The objectives were achieved in simulated conditions of growing pot experiment. We used agricultural soil from the location of Výcapy - Opatovce for the realization of the experiment. The sludge, which was added at various doses, was taken from Central Spis area from locality of Rudňany near the village where minedironore that contains mainly cooper and mercury during last few decades was. We used three types of cereals: barley ( Hordeum sativum L . ) variety PRESTIGE, spring wheat ( Triticum aestivum L . ) variety ISJARISSA and oat ( Avena sativa L . ) variety TATRAN. The length of growing season was 90 days. From the obtained results of two years can be concluded that the accumulation of cooper by seed follows barley ˂ oat ˂ wheat. Even though that the barley is characterized by the highest accumulation of cooper in the seeds, the content did not exceed the maximum level specified by The Codex Alimentarius of the Slovak Republic (CA SR). The results shows that the suitable cultivation of the cereals in localities, which are contaminated with heavy metals, especially by cooper, that the high content of cooper in soil do not pose a risk of accumulation of the metal into the cereal grain.

The content of total polyphenols in different varieties of Solanum tuberosum grow in Spiš area.

essential source of polyphenol compounds. Phenolic compounds are the predominant antioxidants in nutrition and their study is currently being paid much attention. These antioxidants act synergistically; polyphenol compounds protect vitamin C and β-carotene, which in turn helps to increase the effect of vitamin E. Potatoes are very popular vegetables in Slovakia, not only in terms that they are easy to prepare, but also by the fact that they combine the wholesomeness of cereals and delicacy and characteristic chemical composition of vegetables. It is important that they find their place in our diet. Nutritional value of potatoes is determined by the content of nutrients such as protein, starch, fat, minerals, and absence of toxins, as well as by a significant content of bioactive components from the group of polyphenols. The study was performed in order to analyse 7 Slovak potato varieties from Spis area, according to biologically active compounds: such as polyphenols. The content of total polyphenols was determined by the method of Lachman et al., (2003). The lowest determined content of total polyphenol (mg.kg -1 dry matter) in locality Spisský Stvrtok was measured in a variety Victoria (795.05 mg.kg -1 dry matter) and the highest content of total polyphenols in locality Spisský Stvrtok was measured in variety Laura (1238.42 mg.kg -1 dry matter). In the locality Odorin was determine the lowest content of total polyphenols in variety Red Anna (974.09 mg.kg -1 dry matter) and the highest content of total polyphenols was determined in variety Laura (978.95 mg.kg -1 dry matter). Between all varieties in locality Spisský Stvrtok was confirmed the statistically significant difference in the influence of the variety in the contents of total polyphenols (mg.kg -1 DM). This varietal dependence was not appear in samples taken in the locality Odorin. The total polyphenols content of the potatoes can be influenced by other factors, for example locality. In this case, there were statistically significant differences in the content of total polyphenols in variety Laura obtained from two different localities.

The heavy metals content in wild growing mushrooms from burdened Spiš area

In this work, we evaluated the rate of entry of heavy metals into the edible parts of wild mushrooms, from central Spis area. The area is characterized by extremely high content of heavy metals particularly mercury in abiotic and biotic components of ecosystems. The toxicity of heavy metals is well known and described. Known is also the ability of fungi to accumulate contaminants from substrates in which mushrooms grow. We have collected commonly consumed species of mushrooms ( Russula vesca., Macrolepiota procera, Lycoperdon pyriforme, Lecinum piceinum, Boletus reticulatus ). Sampling was conducted for two years 2012 and 2013. The samples taken mushrooms and substrates on which to grow, we determined heavy metal content (Cd, Pb, Cu), including total mercury content modified by atomic absorption spectrometry (AMA - 254). In the substrate, we determined the humus content and pH value. The heavy metal content in soils were evaluated according to Law no. 220/2004 Z.z The exceedance limit values of Cd, Pb, Cu and Hg was recorded. Most significantly the respective limit was recorded in soil samples in the case of mercury. The determined concentration Hg was 39.01 mg.kg -1 . From the results, we evaluated the degree of ability to bioaccumulate heavy metals different kinds of fungi. We also evaluated the health safety of the consumption of these fungi on the comparison with the limit values provided in the food code of SR. We recorded a high rate of accumulation of mercury in the species Boletus reticulatus and Macrolepiota procera. For these types we recorded the most significant than allowed concentrations of mercury in mushrooms. The highest recorded concentration reached 17.64 mg.kg -1 Hg in fresh matter. The limit value was exceeded also in the case of copper. We do not recommend to increased consumption of wild mushrooms in the reference area.