Miroslav Nečas

Matrix Metalloproteinase-2 Promoter Genotype as a Marker of Cutaneous T-Cell Lymphoma Early Stage

The aim of the study was to investigate the DNA polymorphic genotype in MMP-2 promoter gene as a potential candidate region for the development of the cutaneous T-cell lymphoma (CTCL) and/or its progression. A total of 89 Czech patients with CTCL (including 23 patients with large plaque parapsoriasis) were compared to 198 controls of similar age and sex distribution, without personal or family history of chronic skin diseases and without personal history of malignancy. The three selected polymorphisms in the promoter of MMP-2 gene (−1575G/A, −1306C/T, and −790T/G) were determined using the PCR-based methodology with RFLP. In our cohort, the associated GGCCTT MMP-2 promoter genotype was highly significantly more frequent in CTCL-Ia stage patients compared to patients with parapsoriasis, the tests having high sensitivity and specificity (78%, 83%, resp.). To conclude, use of associated MMP-2 promoter genotype as a DNA marker might make it possible to distinguish between the patients with parapsoriasis and those with CTCL stage Ia, which could substantially improve possibilities of clinical diagnostics, therapy design, and prognosis of this serious condition in the early stages.

Contact sensitization in patients with lower extremity dermatitis in the South Moravian region, Czech Republic

Objectives The aim of the study was to determine the frequency of contact sensitization in patients with lower extremity dermatitis. Methods Between the years 2001 and 2007, the authors investigated 462 patients (mean age 49.1 years, 196 men and 266 women) with the eczema/dermatitis localized on their lower extremities, including feet. The patients were investigated with epicutaneous tests of the European Standard Series and also with other special patch tests. Results The most frequent allergens were balsam of Peru, 44/462 (9.5%); wool alcohols, 41/462 (8.9%); nickel sulphate, 39/462 (8.4%); propolis, 35/462 (7.6%); fragrance mix, 34 (7.4%) and colophony, 29/462 (6.3%). Conclusions In patients with lower extremity dermatitis the frequency of contact sensitization is still high, and therefore investigation with epicutaneous tests should belong to the routine dermatological diagnostic procedure in these patients.

Prospective Controlled Study Evaluating Pull Test with Correlation Serum Iron, Ferritin, Tranferin, Vitamin B12, Folic Acid in Patients with Alopecias in The Czech Republic

Hair has not only socio-communication function, but it is also a barometer of the human psyche and the internal environment of the body. Hair may very well reflect the long-term condition of mental frame of mind, but also the general health status of the individuals. The main objective of the study was to identify possible links between pull test and some pathological, biochemical and haematological parameters – serum iron, ferritin, tranferin, vitamin B12, folic acid in patients with alopecia areata, telogen deffluvium and androgenetic alopecias in The Czech Republic

Periorbital Allergic Contact Dermatitis - The Most Common Allergens in the Czech Republic

Aims: 1) To determine actual frequency of sensitization to the most common contact allergens present in ophtalmic products. 2) To assess clinical relevance of the results and detect sources of sensitization. Methods: A total number of 1881 patients (mean age 46.5 years, 357 men, 1,524 women) with periorbital dermatitis underwent patch tested between the years 2001 and 2016 and were retrospectively enrolled into our study. Results: The highest frequency of sensitization was found with thimerosal (9.7%), followed by neomycin sulfate (2.6%), benzalkonium chloride (1.3%), and parabens (0.6%). In terms of clinical relevance however the most important allergen was neomycin sulfate. Conclusion: The most important allergens in ophtalmolog-ic products in our study were neomycin sulfate followed by benzalkonium chloride. With every case of periorbital dermatitis patch tests should be considered, not only with commercial test allergens but with patients actual ophtalmic preparations as well.