Mirosław Pysz
University of Agriculture, Faisalabad
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Featured researches published by Mirosław Pysz.
PLOS ONE | 2016
Ewa Piątkowska; Aneta Kopeć; Renata Bieżanowska-Kopeć; Mirosław Pysz; Aneta Koronowicz; Sylwester Smoleń; Łukasz Skoczylas; Iwona Ledwożyw-Smoleń; Roksana Rakoczy; Edyta Maślak
Iodine is one of the trace elements which are essential for mammalian life. The major objective of iodine biofortification of plants is to obtain food rich in this trace element, which may increase its consumption by various populations. Additionally, it may reduce the risk of iodine deficiency diseases. In this research for the first time we have assessed the bioavailability of iodine from raw or cooked carrot biofortified with this trace element on iodine concentration in selected tissues and various biochemical parameters as well as mRNA expression of some genes involved in iodine metabolism in Wistar rats. Statistically, a significantly higher iodine level was determined in urine, faeces and selected tissues of rats fed a diet containing biofortified raw carrot as compared to a diet without iodine and a diet containing control cooked carrot. Biofortified raw carrot significantly increased triiodothyronine concentration as compared to animals from other experimental groups. The highest thyroid stimulating hormone level was determined in rats fed control cooked carrots. mRNA expression of selected genes was affected by different dietary treatment in rats’ hearts. Biofortified raw and cooked carrot could be taken into account as a potential source of iodine in daily diets to prevent iodine deficiency in various populations.
PLOS ONE | 2016
Aneta Koronowicz; Aneta Kopeć; Adam Master; Sylwester Smoleń; Ewa Piątkowska; Renata Bieżanowska-Kopeć; Iwona Ledwożyw-Smoleń; Łukasz Skoczylas; Roksana Rakoczy; Teresa Leszczyńska; Mirosław Pysz
Although iodization of salt is the most common method used to obtain iodine-enriched food, iodine deficiency disorders are still a global health problem and profoundly affect the quality of human life. Iodine is required for the synthesis of thyroid hormones, which are crucial regulators of human metabolism, cell growth, proliferation, apoptosis and have been reported to be involved in carcinogenesis. In this study, for the first time, we evaluated the effect of iodine-biofortified lettuce on transcriptomic profile of Caco-2 cancer cell line by applying the Whole Human Genome Microarray assay. We showed 1326 differentially expressed Caco-2 transcripts after treatment with iodine-biofortified (BFL) and non-fortified (NFL) lettuce extracts. We analysed pathways, molecular functions, biological processes and protein classes based on comparison between BFL and NFL specific genes. Iodine, which was expected to act as a free ion (KI-NFL) or at least in part to be incorporated into lettuce macromolecules (BFL), differently regulated pathways of numerous transcription factors leading to different cellular effects. In this study we showed the inhibition of Caco-2 cells proliferation after treatment with BFL, but not potassium iodide (KI), and BFL-mediated induction of mitochondrial apoptosis and/or cell differentiation. Our results showed that iodine-biofortified plants can be effectively used by cells as an alternative source of this trace element. Moreover, the observed differences in action of both iodine sources may suggest a potential of BFL in cancer treatment.
International Journal of Food Sciences and Nutrition | 2016
Renata Bieżanowska-Kopeć; Mirosław Pysz; Aneta Kopeć; Sylwester Smoleń; Aneta Koronowicz; Ewa Piątkowska; Roksana Rakoczy; Łukasz Skoczylas; Teresa Leszczyńska
Abstract Research covered six variants: control, unfertilized carrots and carrots fertilized with: KIO3, Na2SeO4, Na2SeO3, KIO3 and simultaneously with Na2SeO4, and fertilized with KIO3 and simultaneously Na2SeO3. Carrots enriched with iodate or selenite, or both iodate and selenite, were characterized by higher amount of these minerals. Changes to the content of micro- and macroelements, during the cooking time of the carrots, both in peeled and unpeeled carrots, did not head in the same direction (increase, decrease and no change). However, cooking an unpeeled carrot generally resulted in the increased content of polyphenol and carotenoids. On the other hand, cooking peeled carrots led to a decrease in the content of polyphenol and a general lack of change in carotenoid content in relation to the unpeeled cooked carrot. During cooking, the antioxidant activity of the carrot being assessed changed together with the direction of changes in polyphenol content but not in line with the direction of changes in carotenoids.
Folia Horticulturae | 2017
Renata Bieżanowska-Kopeć; Sylwester Smoleń; Mirosław Pysz; Aneta Kopeć; Ewa Piątkowska; Roksana Rakoczy; Aneta Koronowicz; Łukasz Skoczylas; Teresa Leszczyńska
Abstract Carrot is a vegetable that contains many nutrients and has strong antioxidant activity as well as pro-health potential. The level of bioactive compounds is strongly connected with the production chain. The thermal treatment of food products induces several biological, physical and chemical changes. In this study, changes in the levels of iodine, total carotenoids, total polyphenols as well as the antioxidant activity of unpeeled and peeled controls and carrots biofortified with (potassium) iodine (KJ) during cultivation due to the cooking and steaming process were investigated. The use of thermal processes resulted in a lower concentration of iodine in the roots of the control as well as in carrots biofortified with (potassium) iodine. In addition, peeling carrots caused higher losses of this trace element in the control and the biofortified carrots cooked or steamed for various times. In this study, a significant growth of the total carotenoids in peeled carrots biofortified with (potassium) iodine and of the total polyphenols in unpeeled carrots biofortified with (potassium) iodine under the influence of the cooking and steaming processes was observed compared with raw peeled and unpeeled biofortified carrots, respectively. Antioxidant activity significantly increased in the unpeeled and peeled carrots biofortified with (potassium) iodine under all thermal treatments in comparison with the raw unpeeled and peeled biofortified carrots.
Journal of Functional Foods | 2015
Aneta Kopeć; Ewa Piątkowska; Renata Bieżanowska-Kopeć; Mirosław Pysz; Aneta Koronowicz; Sylwester Smoleń; Roksana Rakoczy; Łukasz Skoczylas; Teresa Leszczyńska; Iwona Ledwożyw-Smoleń
Journal of Food Process Engineering | 2016
Edyta Maja Kutyła-Kupidura; Marek Sikora; Magdalena Krystyjan; Anna Dobosz; Stanisław Kowalski; Mirosław Pysz; Piotr Tomasik
Acta Scientiarum Polonorum-hortorum Cultus | 2015
Sylwester Smoleń; L Skoczylas; Roksana Rakoczy; Iwona Ledwożyw-Smoleń; Aneta Kopeć; E Piatkowska; Renata Bieżanowska-Kopeć; Mirosław Pysz; Aneta Koronowicz; Włodzimierz Sady
Acta Scientiarum Polonorum Technologia Alimentaria | 2014
Aneta Kopeć; Barbara Borczak; Mirosław Pysz; Elżbieta Sikora; Marek Sikora; Duška Ćurić; Dubravka Novotni
Acta Scientiarum Polonorum-hortorum Cultus | 2015
Sylwester Smoleń; L Skoczylas; Roksana Rakoczy; Iwona Ledwożyw-Smoleń; Marta Liszka-Skoczylas; Aneta Kopeć; E Piatkowska; Renata Bieżanowska-Kopeć; Mirosław Pysz; Aneta Koronowicz; Włodzimierz Sady
Zywnosc-nauka Technologia Jakosc | 2013
Barbara Bugaj; Teresa Leszczyńska; Mirosław Pysz; Aneta Kopeć; Joanna Pacholarz; Katarzyna Pysz-Izdebska