Miryan Margot Sánchez-Jiménez

Identification of the virB operon genes encoding the type IV secretion system, in Colombian Brucella canis isolates.

Canine brucellosis is a zoonotic disease caused by Brucella canis. The establishment of intracellular replicative niches of B. canis is mediated by proteins secreted by the type IV secretion system, which is encoded by the virB operon. The characterization of such genes has been conducted in other species of the genus, but not in B. canis. We report the design of a multiplex PCR test for the detection of the virB operon genes of B. canis. Primers for each of the 12 genes were designed and evaluated using bioinformatics tools. A multiplex PCR assay was standardized and applied to 36 isolates obtained from infected dogs of Aburrá Valley kennels, as well to the Brucella abortus, Brucella melitensis, Brucella suis and Brucella ovis DNA strains. As a result of the in silico design, a pair of primers for each gene was selected. All species and isolates evaluated showed evidence of the presence of the entire virB operon.

Comparison of Brucella canis genomes isolated from different countries shows multiple variable regions.

Brucella canis is a pathogenic bacterium for dogs and its zoonotic potential has been increasing in recent years. In this study, we report the sequencing, annotation and analysis of the genome of Brucella canis strain Oliveri isolated from a dog in a breeding kennel in Medellín, Colombia, South America. Whole genome shotgun sequencing was carried out using the ROCHE 454 GS FLX Titanium technology at the National Center for Genomic Sequencing-CNSG in Medellin, Colombia. The assembly procedure was performed using Newbler v2.6. In the genome annotation process, each contig was analyzed independently using as reference Brucella suis ATCC 1330 chromosomes. This new genome could be useful for the development of diagnostic tools and for vaccines search as well, in order to reduce the health impact of this infection in both, dogs and humans. The sequence was deposited in EMBL-EBI with accession numbers HG803175 and HG803176 for chromosomes 1 and 2, respectively.

Factors associated with Brucella canis seropositivity in kennels of two regions of Antioquia, Colombia

El objetivo fue determinar la seroprevalencia a Brucella canis en perros y humanos convivientes en criaderos caninos y explorar los factores de riesgo asociados a la seropositividad. Se tomaron 20 criaderos, en los cuales se realizo diagnostico serologico por PARP-2ME de 428 caninos y 91 humanos. Se aplico una encuesta para determinar los factores de riesgo y se analizaron los datos mediante regresion logistica. Se determino una seroprevalencia de 15% en caninos y 9% en humanos convivientes. Se determinaron como factores asociados a la seropositividad canina el historial de seropositividad canina, conservar los caninos seropositivos, historial de aborto, higiene y proteccion del operario deficientes durante el servicio reproductivo, y procedimiento inseguro durante la atencion de abortos. Como factores protectores se establecieron la ubicacion rural de los criaderos, facilidad de aseo de los caniles, PARP-2ME premonta, y procedimiento seguro durante la atencion de partos. En humanos se determinaron factores asociados: criaderos ubicados en el Valle Aburra y de tipo urbano.The objectives of this study were to determine Brucella canis seroprevalence in dogs and in humans living near kennels and to explore risk factors associated with seropositivity. Twenty kennels were included in a serological survey with RSAT-2ME, and samples were collected from 428 dogs and 91 humans. An interview was applied to determine risk factors, and the data were analyzed using logistic regression. Seroprevalence was 15% in dogs and 9% in humans. Factors associated with current canine seropositivity were: history of canine seropositivity, non-culling of seropositive dogs, history of abortion, poor hygiene and personal protection during reproductive service, and unsafe procedures during care for abortions. Protective factors included: rural location of kennels, ease of cleaning kennels, pre-mating RSAT-2ME, and safe procedures during care for delivery. Factors associated with seropositive status in humans were: kennels located in Valle de Aburrá and urban location.

Factores asociados con la seropositividad a Brucella canis en criaderos caninos de dos regiones de Antioquia, Colombia

El objetivo fue determinar la seroprevalencia a Brucella canis en perros y humanos convivientes en criaderos caninos y explorar los factores de riesgo asociados a la seropositividad. Se tomaron 20 criaderos, en los cuales se realizo diagnostico serologico por PARP-2ME de 428 caninos y 91 humanos. Se aplico una encuesta para determinar los factores de riesgo y se analizaron los datos mediante regresion logistica. Se determino una seroprevalencia de 15% en caninos y 9% en humanos convivientes. Se determinaron como factores asociados a la seropositividad canina el historial de seropositividad canina, conservar los caninos seropositivos, historial de aborto, higiene y proteccion del operario deficientes durante el servicio reproductivo, y procedimiento inseguro durante la atencion de abortos. Como factores protectores se establecieron la ubicacion rural de los criaderos, facilidad de aseo de los caniles, PARP-2ME premonta, y procedimiento seguro durante la atencion de partos. En humanos se determinaron factores asociados: criaderos ubicados en el Valle Aburra y de tipo urbano.The objectives of this study were to determine Brucella canis seroprevalence in dogs and in humans living near kennels and to explore risk factors associated with seropositivity. Twenty kennels were included in a serological survey with RSAT-2ME, and samples were collected from 428 dogs and 91 humans. An interview was applied to determine risk factors, and the data were analyzed using logistic regression. Seroprevalence was 15% in dogs and 9% in humans. Factors associated with current canine seropositivity were: history of canine seropositivity, non-culling of seropositive dogs, history of abortion, poor hygiene and personal protection during reproductive service, and unsafe procedures during care for abortions. Protective factors included: rural location of kennels, ease of cleaning kennels, pre-mating RSAT-2ME, and safe procedures during care for delivery. Factors associated with seropositive status in humans were: kennels located in Valle de Aburrá and urban location.

Infección por Brucella canis en humanos: propuesta de un modelo teórico de infección a través de la ruta oral

Resumen La infeccion por Brucella canis en los humanos se ha reconocido recientemente como una zoonosis, pero frecuentemente es sub reportada debido a que los sintomas pueden confundirse con los de un resfriado comun u otras infecciones causadas por otros patogenos. Los caninos son los hospederos primarios de Brucella canis ; el incremento en la tendencia de tener perros como mascotas podria tambien aumentar la posibilidad de transmision de la infeccion a los humanos por el estrecho contacto entre la mascota infectada y su propietario. En Colombia, hay reportes de aislamientos de B. canis de caninos de criaderos y de un humano en contacto con perros infectados, al igual que reportes de caninos seropositivos a la infeccion. Sin embargo, no hay mucha informacion disponible sobre los mecanismos de interaccion hospedero-patogeno que conduzcan al establecimiento de la infeccion por Brucella canis en perros y en humanos esta informacion es todavia menor. En esta revision se propone un modelo para la infeccion humana con Brucella canis a traves de la ruta oral utilizando la informacion disponible para otras especies de Brucella que infectan al humano, incluyendo B. abortus y B. melitensis, que difieren de B. canis en la composicion estructural de su lipopolisacarido. Tambien se hipotetiza el mecanismo de infeccion celular que es usado por B. canis para invadir y establecer la infeccion en celulas no fagociticas y fagociticas.

Fatores associados à soropositividade por Brucella canis em cães reprodutores em duas regiões no Estado de Antioquia, Colômbia

El objetivo fue determinar la seroprevalencia a Brucella canis en perros y humanos convivientes en criaderos caninos y explorar los factores de riesgo asociados a la seropositividad. Se tomaron 20 criaderos, en los cuales se realizo diagnostico serologico por PARP-2ME de 428 caninos y 91 humanos. Se aplico una encuesta para determinar los factores de riesgo y se analizaron los datos mediante regresion logistica. Se determino una seroprevalencia de 15% en caninos y 9% en humanos convivientes. Se determinaron como factores asociados a la seropositividad canina el historial de seropositividad canina, conservar los caninos seropositivos, historial de aborto, higiene y proteccion del operario deficientes durante el servicio reproductivo, y procedimiento inseguro durante la atencion de abortos. Como factores protectores se establecieron la ubicacion rural de los criaderos, facilidad de aseo de los caniles, PARP-2ME premonta, y procedimiento seguro durante la atencion de partos. En humanos se determinaron factores asociados: criaderos ubicados en el Valle Aburra y de tipo urbano.The objectives of this study were to determine Brucella canis seroprevalence in dogs and in humans living near kennels and to explore risk factors associated with seropositivity. Twenty kennels were included in a serological survey with RSAT-2ME, and samples were collected from 428 dogs and 91 humans. An interview was applied to determine risk factors, and the data were analyzed using logistic regression. Seroprevalence was 15% in dogs and 9% in humans. Factors associated with current canine seropositivity were: history of canine seropositivity, non-culling of seropositive dogs, history of abortion, poor hygiene and personal protection during reproductive service, and unsafe procedures during care for abortions. Protective factors included: rural location of kennels, ease of cleaning kennels, pre-mating RSAT-2ME, and safe procedures during care for delivery. Factors associated with seropositive status in humans were: kennels located in Valle de Aburrá and urban location.