Mitsumasa Matsuda

Kenji Ito; Masanobu Yoshikawa; Miho Maeda; Xing Lu Jin; Shigeru Takahashi; Mitsumasa Matsuda; Raita Tamaki; Hiroyuki Kobayashi; Toshiyasu Suzuki; Atsushi Hashimoto

Our recent study has shown that the intracerebroventricular administration of d-serine, an endogenous and selective agonist for the glycine site of the N-methyl-d-aspartate receptor, alone or in combination with morphine, leads to the potentiation of antinociception on the tail-flick response. Although there is a variety of information concerning the effects of benzodiazepines on opioid-induced antinociception, little is known about the effect of benzodiazepines on the N-methyl-d-aspartate receptor agonist-induced antinociception. To clarify the analgesic interactions among the benzodiazepine/GABA(A), N-methyl-d-aspartate and opioid receptors at the supraspinal level, we investigated the effects of intracerebroventricular administration of midazolam, a benzodiazepine receptor agonist, on the antinociception evoked by the intracerebroventricular application of d-serine or morphine. The intracerebroventricular administration of midazolam alone produced hyperalgesia on the tail-flick response in a benzodiazepine receptor antagonist, flumazenil-reversible manner. The antinociception induced by the intracerebroventricular application of d-serine or morphine was attenuated by the intracerebroventricular administration of midazolam. In addition, this inhibitory effect of midazolam on the antinociception of d-serine or morphine was antagonized by the intracerebroventricular administration of flumazenil. Together with the facts that d-serine and midazolam act as selective agonists for the glycine site of the N-methyl-d-aspartate receptor and benzodiazepine/GABA(A) receptor, respectively, these observations suggest a functional interaction between the NMDA and benzodiazepine/GABA(A) receptors in the regulation of antinociception at the supraspinal level.

Masanobu Yoshikawa; Kenji Ito; Miho Maeda; Kazuhito Akahori; Shigeru Takahashi; Xing Lu Jin; Mitsumasa Matsuda; Toshiyasu Suzuki; Tetsuo Oka; Hiroyuki Kobayashi; Atsushi Hashimoto

Junichi Nishiyama; Mitsumasa Matsuda; Satoko Ando; Miyoko Hirasawa; Toshiyasu Suzuki; Hiroyasu Makuuchi

Mitsumasa Matsuda; Toshiyasu Suzuki

Miho Ito; Masanobu Yoshikawa; Kenji Ito; Mitsumasa Matsuda; Xing Lu Jin; Shigeru Takahashi; Hiroyuki Kobayashi; Toshiyasu Suzuki

Mitsumasa Matsuda; Junichi Nishiyama; Miho Maeda; Toshiyasu Suzuki

Mitsumasa Matsuda; Masanobu Yoshikawa; Takugi Kan; Mariko Watanabe; Junko Ajimi; Shigeru Takahashi; Masaaki Miura; Kenji Ito; Hiroyuki Kobayashi; Toshiyasu Suzuki

Mitsumasa Matsuda; Kenji Ito; Junichi Nishiyama; Toshiyasu Suzuki

Mitsumasa Matsuda

Mitsumasa Matsuda; Masaaki Miura; Hiroko Ito; Kyoko Mizusawa; Yumi Harayama; Toshiyasu Suzuki