Mitsuru Nakahara

Immunohistochemical study of overexpression of fibroblast growth factor-1 (FGF-1), FGF-2, and FGF receptor-1 in human malignant salivary gland tumours.

Fibroblast growth factor‐1 (FGF‐1) and FGF‐2 are broad spectrum mitogens. The expression of FGF‐1, FGF‐2, and their receptor, FGF receptor‐1 (FGFR‐1), was examined in malignant salivary gland tumours and normal salivary glands, using immunohistochemical methods. In seven cases of adenoid cystic carcinoma (ACC), both duct‐like cells and modified myoepithelial cells were apparently immunopositive for FGF‐1, FGF‐2, and FGFR‐1. In five cases of mucoepidermoid carcinoma (MC), all three types of tumour cells including epidermoid cells, mucous cells, and intermediate cells expressed immunoreactive FGF‐1, FGF‐2, and FGFR‐1. In these malignant salivary gland tumours, increased expression of FGFR‐1 correlated with the intensity of both FGF‐1 and FGF‐2 immunoreactivity. In contrast to malignant salivary gland tumours, eight cases of normal salivary gland showed negative immunostaining for FGF‐1, FGF‐2, and FGFR‐1 while four cases were weakly immunoreactive for FGF and its receptor. These results demonstrate that malignant salivary gland tumours overexpress FGF‐1, FGF‐2, and FGFR‐1 compared with normal salivary glands and suggest that these growth factors may play an important role in facilitating neoplastic progression in human salivary glands.

Expression of the insulin-like growth factor system and cancer progression in hormone-treated prostate cancer patients

Abstract Background : The insulin‐like growth factor (IGF) system, which is composed of two ligands (IGF‐I and IGF‐II), two receptors (IGFR‐I and IGFR‐II) and six binding proteins (IGFBP‐1 to ‐6), plays an important role in cell biology. To identify the main components in the IGF system that contribute to human prostate cancer progression after hormone therapy, mRNA expression of the IGF system in human prostate cancer tissue was systematically examined.

HORSESHOE KIDNEY WITH RETROCAVAL URETER AND URETEROPELVIC JUNCTION OBSTRUCTION : A CASE REPORT

We report a rare disorder, horseshoe kidney accompanied by retrocaval ureter and ureteropelvic junction obstruction, in a 42‐year‐old man. This is the third case in the world literature.

Establishment of a SV40-transformed cell line from primary culture of rat dorsolateral prostatic epithelial cells

A permanent cell line of the rat dorsolateral prostate epithelium was established after transformation of primary cultured cells with simian virus 40 (SV40). The established cells had SV40 T-antigen in their nuclei but lacked such typical characteristics of transformed cells as piled-up growth. They grew in a monolayer with an epithelial morphology, were stained with antikeratin antisera, and also retained an ability to form dome-like structure at a confluent state.

Androgen receptor in electroresected and cold punch-resected specimens Usefulness of Kaplan cold punch resectoscope

The measurement of androgen receptor and 5 alpha-dihydrotestosterone (DHT) levels in prostatic carcinoma may be of value in predicting responsiveness to anti-androgenic therapy. A sufficient amount of prostatic carcinoma tissue must be removed for measuring androgen receptor and DHT levels. We have studied the usefulness of Kaplan cold punch-resection compared with electroresection for obtaining tissue in 29 cases of enucleated benign prostatic hyperplasia. DHT level in electroresected specimens was similar to controls. However, following low, moderate, and high-power electroresection an average of total R1881 binding sites (Bmax) of electroresected specimens was reduced to 82.2, 71.9, and 52.1 per cent of corresponding controls, respectively. In contrast, the per cent decrease of Bmax of Kaplan cold punch-resected specimens was only 10 per cent of the control. From our data, Kaplan cold punch-resection of the prostate appears to be a useful tool for obtaining tissue suitable for measuring androgen receptor levels.

Immunohistochemical Localization of Testosterone-Estradiol-Binding Globulin in a Clinically Hormone-Independent Prostatic Carcinoma

The localization of the testosterone-estradiol-binding globulin (TeBG) in prostate, liver and metastatic lymph node cells of a patient with hormone-independent prostatic carcinoma was studied by the t

Purification of Human Testosterone-Estradiol Binding Globulin (TeBG): A Specific RIA of Human TeBG

The immunological properties of human serum testosterone-estradiol binding globulin (TeBG) which was purified by affinity chromatography and hydroxyapatite column chromatography were investigated and a specific radioimmunoassay (RIA) for human TeBG was established. The purified TeBG here obtained was 86,000 in molecular weight and the antibody prepared against this TeBG slightly cross-reacted with human immunoglobulin G (IgG). The human TeBG labeled with 125I had a high binding activity to the antihuman TeBG antibody, and the sensitivity of the RIA thus obtained was enough to detect the TeBG in 2 μl of the serum.

Body mass index as a classifier to predict biochemical recurrence after radical prostatectomy in patients with lower prostate-specific antigen levels

Prostate cancer, one of the most common malignant tumors among men, is closely associated with obesity and, thus far, several studies have suggested the association between obesity and aggressive pathological characteristics in the United States. However, the effect of obesity on prostate cancer mortality is controversial, and it remains unclear whether obesity contributes to the aggressiveness of prostate cancer in Asian patients. The aim of the present study was to investigate the association between body mass index (BMI) and the clinicopathological characteristics of prostate cancer in 2,003 Japanese patients who underwent radical prostatectomy. There was a significant association between higher BMI and higher Gleason score (GS). The multivariate analysis also revealed that BMI was an independent indicator for GS ≥8 at surgery. Moreover, among patients with lower prostate-specific antigen levels, biochemical recurrence-free survival was significantly worse in those with higher BMI. These results suggest that BMI may be a classifier for predicting adverse pathological findings and biochemical recurrence after radical prostatectomy in Japanese patients.