Mogens Lykkegaard Nielsen

Trimethoprim in human prostatic tissue and prostatic fluid.

Following the administration of trimethoprim (TMP) by mouth in doses varying from 5.0 to 8.1 mg TMP per kg body weight per 24 hours over a period of 24–48 hours, the concentration was examined in either the prostatic tissue or the prostatic secretion, in a total of 13 patients. A microbiological method was employed to determine the concentration, using B. ptrmilis as test organism. A method of collecting the prostatic secretion was used which excluded the admixture of urine with higher antibiotic content than that of the secretion. Tissue was examined which was obtained both by open and closed (transperineal) biopsy. The concentrations of TMP found in both the secretion and the tissue (9–12 hours after the last administration of TMP) were at a thera-peutically active level (≥MIC × 5) with respect to the majority of those bacteria usually occurring in urinary tract infection.

Studies on the Pathology of Prostatitis: A Search for Prostatic Infections with Obligate Anaerobes in Patients with Chronic Prostatitis and Chronic Urethritis

Forty-six patients with chronic prostatitis or chronic urethritis were examined for anaerobic infections in the urine and in the prostate. The patients underwent a thorough urological examination. Anaerobic and aerobic cultures were made, both from transperineal biopsy material and from urine and prostatic secretion. The technique of anaerobic culture was based on a transport time for the clinical samples of less than one hour, as well as on the use of the “glove-box” technique and prereduced media, Anaerobic bacteriuria was found in 1 out of 46 patients, but in no case were anaerobic bacteria localized with certainty to the prostatic tissue or the prostatic secretion. No anaerobic bacteria were found in the anterior urethra.

Excretion of Metronidazole in Human Bile

Excretion of metronidazole (MNZ) in the normal and in the diseased biliary tract was investigated in 58 patients after oral or intravenous administration of MNZ. After oral administration MNZ appeared rapidly in hepatic bile, and throughout the period of absorption and elimination almost identical concentrations of MNZ were found in serum and hepatic bile. After intravenous administration no significant differences were found between concentrations of MNZ in common duct bile and serum in the non-obstructed common duct; in common duct obstruction, concentrations of MNZ in common duct bile were 56–99 per cent of corresponding concentrations in serum. MNZ was concentrated in normal gallbladders. In patients with gallbladder stones and preserved function of the gallbladder and in patients with no function of the gallbladder but a patent cystic duct, no significant differences were found between concentrations of MNZ in gallbladder bile, common duct bile, and serum. In most gallbladders with the cystic duct bl...

Inflammatory Changes in the Non-Infected Prostate Gland. A Clinical, Microbiological and Histological Investigation

n 36 patients (18 with chronic prostatitis and 18 with chronic urethritis) were studied to determine the incidence and nature of prostatic inflammatory changes in patients with clinically diagnosed chronic prostatitis and chronic urethritis without the occurrence of aerobic and anaerobic bacteria, Mycoplasms, and fungi or viruses in the prostatic tissue or prostatic secretion. Excretory urography (IVP), cytoscopy and urethroscopy were performed on all patients. Prostatic biopsy was done transperineally by the method of Veenema. None of the biopsy specimens exhibited acute inflammatory changes. Chronic inflammation in the form of infiltration around the acini with mononuclear inflammatory cells occurred without simultaneous infection in 17 cases. Changes were slight in 11 cases, moderate in 5 and severe in 1. No relationship was found between changes in the appearance of epithelium and occurrence of inflammatory changes. Causes of inflammatory reaction is not known. Current research is being done to determine possible cell-bound hypersensitivity using lymphocyte migration technique.n

Anaerobic infections in chronic prostatitis and chronic urethritis

Abstract46 patients with chronic prostatitis or chronic urethritis were examined for anaerobic infections in the urine and in the prostate. The patients underwent a thorough urological examination. Anaerobic and aerobic cultures were made, both from transperineal biopsy material and from urine and prostatic secretion. The technique of anaerobic culture was based on a transport time for the clinical samples of less than 1/2 h, as well as on the use of the “glove-box” technique and pre-reduced media. Anaerobic bacteriuria was found in 1 out of 46 patients, but in no case were anaerobic bacteria localized with certainty to the prostatic tissue, the prostatic secretion or the urethra.

New approach to treatment of severe incisional abscesses following laparotomy. Wound closure under systemic antibiotic cover four days after drainage.

Forty consecutive patients who developed subcutaneous abscesses after intraperitoneal operations were treated by incision and drainage followed by suture of the wound four days later under antibiotic cover. The wound was closed by means of interrupted Prolene® sutures. No sutures were placed in the wound cavity, and no drain was applied. The antibiotic used was clindamycin 600 mg intravenously peroperatively and 150 mg every six hours for four days.

Sterilization by vas occlusion without transection does not reduce postvasectomy sperm-agglutinating antibodies in serum. A randomized trial of vas occlusion versus vasectomy.

The influence of transection of the vasa on the increase in sperm-agglutinating activity in serum after vasectomy was investigated in a prospective randomized study. Sixty-eight males requesting vasectomy were allocated to either of two groups: vas occlusion by tantalum clips without transection of the vasa, or conventional vasectomy by transection and ligation. Sperm-agglutinating antibodies were determined by a micro-agglutination test before and 6 months after vasectomy or vas occlusion. A significant increase in sperm-agglutinating activity in serum after sterilization was found in both groups, but there was no intergroup difference.