Mohamed I. S. Abdelhady
Helwan University
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Featured researches published by Mohamed I. S. Abdelhady.
Plant Molecular Biology | 2009
Mohamed I. S. Abdelhady; Till Beuerle; Dietrich Ober
Homospermidine synthase is the first specific enzyme in the biosynthesis of pyrrolizidine alkaloids. Whereas the substrates putrescine and spermidine are part of the highly dynamic polyamine pool of plants, the product homospermidine is incorporated exclusively into the necine base moiety of pyrrolizidine alkaloids. Recently, the gene encoding homospermidine synthase has been shown to have been recruited several times independently during angiosperm evolution by the duplication of the gene encoding deoxyhypusine synthase. To test whether high levels of homospermidine suffice for conversion, at least in traces, to precursors of pyrrolizidine alkaloids, transgenic tobacco plants were generated expressing homospermidine synthase. Analyses of the polyamine content revealed that, in the transgenic plants, about 80% of spermidine was replaced by homospermidine without any conspicuous modifications of the phenotype. Tracer-feeding experiments and gas chromatographic analyses suggested that these high levels of homospermidine were not sufficient to explain the formation of alkaloid precursors. These results are discussed with respect to current models of pathway evolution.
Pharmaceutical Biology | 2011
Eman G. Haggag; Amel M. Kamal; Mohamed I. S. Abdelhady; Mortada M. El-Sayed; Eman A. El-Wakil; Sayed S. Abd-El-hamed
Context: Cancer is a serious clinical problem to the health care system. Anticancer drugs have been extracted from plants containing phenolic compounds. Leucenia species (Fabaceae) contain a variety of bioactive components of numerous biological and pharmacological properties. Objective: This study explored the constitutive polyphenols of Leucenia leucocephala Lam. growing in Egypt and evaluated the antioxidant and cytotoxic activity. Materials and methods: Chemical structures of the isolated compounds from the leaves of L. leucocephala were established by spectral techniques (UV, 1H, and 13C NMR, MS). Results: Chromatographic separation of 80% MeOH extract of the leaves of L. leucocephala have resulted in a novel flavonoid-galloyl glycoside [myricetin 3-O-(2′,3′4′-tri-O-galloyl)-α-l-rhamnopyranoside] with three known polyphenolic compounds isolated for the first time from this species (apigenin 7-O-β-d-glucuronopyranoside methyl ester, luteolin 7-O-β-d-glucuronopyranoside methyl ester, and 1,3,6-tri-O-galloyl-β-d-glucopyranose) and seven known previously isolated compounds. Also, 80% methanol extract exhibited high antioxidant activity (SC50u2009=u20093.94 µg/ml), which is correlated with its phenolic content. The extract also showed cytotoxic activity against Hep G2 (IC50 value 1.41 µg/ml) confirming its anticancer activity against hepatocellular carcinoma. Among the tested compounds (4–8) for antioxidant property, compound 7 was the most active compound (SC50u2009=u20092.49 µg/ml). Also compounds 7 and 8 exhibited high cytotoxic activity (IC50u2009=u20092.41 and 2.81 µg/ml, respectively). Discussion and conclusion: These findings demonstrate that the leaves of L. leucocephala contain a considerable amount of polyphenolic compounds with high antioxidant properties, thus it has great potential as a source for natural health products.
American Journal of Plant Sciences | 2011
Mohamed I. S. Abdelhady; Amira Abdel Motaal; Ludger Beerhues
Journal of Pharmacy Research | 2013
Eman G. Haggag; Mohamed I. S. Abdelhady; Amel M. Kamal
Polymer Journal | 2012
Mohamed I. S. Abdelhady; Amel M. Kamal; Nashwa F Tawfik; Soad M Abdelkhalik
Biosciences, Biotechnology Research Asia | 2015
Ammar Bader; Mohamed I. S. Abdelhady; Usama Shaheen; Yasser El-Malah; M.A.S Abourehab; Mohamed F. Barghash
BioTechnology: An Indian Journal | 2012
Mohamed I. S. Abdelhady; Amel M. Kamal; N.F.Tawfik; S.M.Abdelkhalik; Amira Abdel Motaal; Ludger Beerhues
Bulletin of Faculty of Pharmacy, Cairo University | 2018
Mohamed I. S. Abdelhady; Amel M. Kamal; Mohamed F. Barghash
Journal of Advanced Pharmacy Research | 2017
Amel M. Kamal; Eman G. Haggag; Mohamed I. S. Abdelhady; Khayrya Youssif; Amany Sleem
Biosciences, Biotechnology Research Asia | 2016
Mohamed I. S. Abdelhady; Amira Abdel Motaal; Rehab Kamel; Omar A. Rashwan