Mohamed Osman

Effect of lippes loop on sperm recovery from human fallopian tubes

Recovery of human sperm from the fallopian tubes after natural insemination have been studied in sixty women; out of these, thirty cases were fitted with the Lippes loop, type C, for at least six months. The mean sperm count/high power field (HPF X 400) and percentage of motile sperm in the cervical mucus in both control and Lippes loop groups showed no significant differences. No sperm were recovered from the fallopian tubes of the Lippes loop group, while fourteen control cases had a positive recovery of sperm from the tubal washings. The mean number of white blood corpuscles in the tubal washings of Lippes loop-fitted cases was found to be significantly higher than in control cases. The phagocytic capacity of these cells may explain the low incidence of sperm recovered from the fallopian tubes in such cases.

The relaxant property of local prostaglandin E2 on the non-pregnant uterus — A cyclic triphasic response

The response of the non-pregnant human uterus to intravenous (i.v.) injections and intra-uterine instillation of various doses of prostaglandin E-2 (PGE-2) was evaluated at the different phases of the menstrual cycle in 13 fertile regularly menstruating women who were neither lactating nor using any hormonal therapy. Uterine contractility was recorded by the microballoon technique in at least three sessions(proliferative, mid-cycle and secretory) in a single cycle with endometrial biopsy performed immediately following the last session to ascertain that the particular cycle was an ovulatory one. Single i.v. injections of PGE-I had a consistent stimulatory effect on the contractility throughout the cycle with a tendency towards a decreased uterine response at mid-cycle and luteal phase as compared to the proliferative part of the cycle. Intra-uterine instillation of the compound induced a peculiar and interesting type of response. In the proliferative and secretory phases of the cycle the response was one of stimulation; being more pronounced in the former period. However, around ovulation time, the local administration induced an evident uterine relaxation in most cases without any instance of stimulation. The possible implication of this triphasic response behaviour on the non-pregnant uterus within certain physiological events and pathological conditions is discussed.

The effect of an oral contraceptive on serum lipids

Abstract The effect of an oral contraceptive containing 3 mg norethisterone acetate and 0.05 mg ethinyl ≐stradiol on serum lipids was studied on a group of 16 women. The determination of different parameters of serum lipids was performed before the intake of pills and six months later, as well as in a control group. When the mean lipid values in the treated group were compared to the pretreatment values, significant increases were noted in the free fatty acids, triglycerides, beta proteins and the beta:alpha lipoprotein ratio while a significant decrease was noted in the percent of cholesterol esterification. Several mechanisms may be responsible for these alterations.

Reduced uterine response to PGF2α under oral contraceptives

Abstract The effect of prostaglandin F2α (PGF2α) on the non-pregnant human uterus was a evaluated in 35 patients. The compound was administered both by intra-uterine and intra-venous routes. Two groups of volunteers were studeid; fifteen cases who were under oral contraceptive and twenty women with normal ovulatory cycles. The uterus under the influence of oral contraceptives showed a reduced response to local and systemic administration fo PGF2α at all phases of the menstrual cycle. The implications of these findings in certain physiological and pathological conditions related to reproduction are discussed.

Aberrant Uterine Response to Prostaglandin E2 as a Possible Etiologic Factor in Functional Infertility

The clinical entity of functional infertility still remains unexplained and largely lacking a line of proper management. The uterine response to prostaglandin E2 (PGE2) and prostaglandin F2alpha (administered by intravenous and intrauterine routes) was evaluated during different phases of the menstrual cycle in five functionally infertile women. The results (quantitative and qualitative) were compared with those elicited among 20 fertile women. There were no apparent differences between the two groups with respect to either compound (systemic or local) when given during the proliferative or luteal phase of the cycle. However, at midcycle, about the time of ovulation (days 13 to 18), there was a salient deviation in the type of response following local instillation of PGE2; in the normal fertile group there was a definite inhibition of motility, while uteri in the functionally infertile group showed marked stimulation. The possible role of this aberrant uterine response in the etiology of functional infertility is discussed.

Uterine response to prostaglandin E2 under oral contraceptives

Abstract The effect of prostaglandin E 2 (PGE 2 ) on the non-pregnant human uterus was evaluated in 35 patients. The compound was administered both by the i.v. and intra-uterine routes. Two groups of volunteers were studied; fifteen cases who were under oral contraceptives and twenty women with normal ovulatory cycles. The latter group served as the control group. The uterus under the influence of oral contraceptives showed a markedly reduced response to i.v. injections of PGE 2 at all phases of the menstrual cycles. However, the response at mid-cycle to local instillation of the compound was contradictory in the two groups, i.e. local intra-uterine instillation of PGE 2 induced uterine stimulation in pill users as compared to the usually observed uterine inhibition in ovulatory (control) cases around mid-cycle. In the proliferative and luteal phases (early and late in the cycle), the response to intra-uterine injections was qualitatively similar in the two groups but greatly reduced quantitatively in the pill user group. The implications of these findings in certain physiological processes and pathological conditions related to reproduction are discussed.

Effect of aspirin on the luteal phase of human menstrual cycle

The effect of aspirin (acetylsalicylic acid) on the luteal phase length and function was studied in ten normal cycling women. They received three grams daily for twenty days starting from the fifth day of the cycle. Urinary pregnanediol-3 alpha-glucuronide (Pg-diol- 3G ) and luteinising hormone (LH) were assayed in daily early morning urine samples together with daily vaginal smear for cytohormonal evaluation. The excretion profile of Pg-diol- 3G and LH of another group of ten normal women were taken as controls. Aspirin caused shortening of both cycle length and luteal phase duration. Available evidence suggests the presence of corpus luteum deficiency in the treated cycles.