Monika Karpińska-Kołaczek

Does climate affect pollen morphology? Optimal size and shape of pollen grains under various desiccation intensity

Seed production is likely constrained by pollen limitation and the viability of pollen grains decreases rapidly in time due to water evaporation. Any decrease in the surface-to-volume ratio, through increase in size or change in shape of a grain, reduces the rate of water loss. However, grain size trade-offs with the number of grains that can be produced by a plant. Here, we tested the hypothesis that under higher desiccation stress pollen grains become larger and more spherical. We analyzed data on the pollen morphology of eight Rosaceae species and the desiccation intensity based on temperature, potential evapotranspiration and altitude. To explain the mechanisms underlying our results, we present a model that optimizes the size and shape of pollen grains under different conditions. We report that pollen grains under more intense desiccation stress during flowering periods tend to be larger but do not change shape. This conclusion is consistent with the results of a theoretical model presented here. Our report fills a gap in our knowledge about a fundamental process in plant reproduction. We also discuss the significance of our results in light of current palynological and ecological problems (e.g., global climate change).

The Late Glacial and Holocene development of vegetation in the area of a fossil lake in the Skaliska Basin (north-eastern Poland) inferred from pollen analysis and radiocarbon dating

ABSTRACT The development of vegetation in the Skaliska Basin has been reconstructed on the basis of palynological analysis and radiocarbon dating (AMS technique) of 6 sites from the late phase of the Bolling- Allerod interstadial complex to modern times. Although the area covers 90 km2, the mosaic character of habitats led to the development of different patterns of vegetation changes during the Late Glacial and Holocene. Only one site located in the eastern part of the Skaliska Basin reflected the ‘pine phase’ of Allerod, and this is the oldest data on vegetation in the Skaliska Basin. Interesting discrepancies were recorded during the Younger Dryas when patches of shrublands with Juniperus were distinct around some of the sites, while steppe with Artemisia was common in others. The beginning of the Holocene brought an expansion of birch-pine forest, but around 9600 cal. BC a cold oscillation took place which was reflected in an increase in birch in the woodlands in the western and eastern part of the Skaliska Basin. In the Preboreal chronozone elm (Ulmus) also expanded in the area but its appearance was non-synchronous. The vegetation of the Boreal chronozone was similar in the whole area and the most characteristic feature was the rapid expansion of hazel (Corylus avellana) which displaced Betula from the most of its sites. At that time a distinct redeposition of pollen material in the Parchatka river valley was detected which was probably the effect of an increase in fluvial activity of the river (humid oscillation). The following stage of vegetation development was climax woodlands with Tilia cordata, Ulmus, Quercus, Corylus avellana, and Alnus in damp places. At the beginning of the Subboreal chronozone the expansion of Quercus took place, which was subsequently replaced by Picea abies and partly Carpinus betulus. The pattern of Picea abies expansion distinctly presents two maxima which is characteristic of many sites in the north-eastern Poland. The Subatlantic chronozone is represented only by the profile from the Skaliski Forest, where, because of sandy ground, Pinus sylvestris was the dominant element. Human impact was poorly reflected through the rare occurrence of pollen grains of Cerealia type in the pollen profiles spanning the time from the Subboreal chronozone to modern times. In most profiles AMS dating produced age discrepancies, which limited the possibility of establishment of a detailed chronology. However, dates obtained from the material contaminated by mixture of glycerine, thymol and ethyl alcohol, pretreated by alcohol, showed reliable results in most cases.

A novel testate amoebae trait-based approach to infer environmental disturbance in Sphagnum peatlands

Species’ functional traits are closely related to ecosystem processes through evolutionary adaptation, and are thus directly connected to environmental changes. Species’ traits are not commonly used in palaeoecology, even though they offer powerful advantages in understanding the impact of environmental disturbances in a mechanistic way over time. Here we show that functional traits of testate amoebae (TA), a common group of palaeoecological indicators, can serve as an early warning signal of ecosystem disturbance and help determine thresholds of ecosystem resilience to disturbances in peatlands. We analysed TA traits from two Sphagnum-dominated mires, which had experienced different kinds of disturbances in the past 2000 years – fire and peat extraction, respectively. We tested the effect of disturbances on the linkages between TA community structure, functional trait composition and functional diversity using structural equation modelling. We found that traits such as mixotrophy and small hidden apertures (plagiostomic apertures) are strongly connected with disturbance, suggesting that these two traits can be used as palaeoecological proxies of peatland disturbance. We show that TA functional traits may serve as a good proxy of past environmental changes, and further analysis of trait-ecosystem relationships could make them valuable indicators of the contemporary ecosystem state.

Palaeobotanical studies on Late Glacial and Holocene vegetation development and transformations of the ‘Wielkie Błoto’ mire near Gołdap (north-eastern Poland)

ABSTRACT This paper presents the results of palynological, macrofossil and peat analyses that were conducted on deposits from a profile collected from the Wielkie Błoto mire near Bałupiany (north eastern Poland). The investigation revealed that the recorded changes of vegetation span the period from the decline of the Younger Dryas (ca 9600 cal. yr BC) to the late Subboreal or early Subatlantic chronozone, but due to a 40 cm long sediment gap a complete reconstruction was not possible. At the beginning, the area was occupied by steppe and tundra communities together with abundant Juniperus stands. A subsequent expansion of birch (Betula) woodlands with pine (Pinus sylvestris) took place in the Preboreal chronozone in which a rise in the water level and/or basin deepening was recorded at the site as well. The domination of such woodlands lasted to the end of the Boreal chronozone when Corylus avellana expanded rapidly. In the Atlantic chronozone multispecies deciduous forests developed with Tilia cordata and Quercus, while Ulmus together with Alnus spread in damp habitats. During this chronozone, traces of the occurrence of Carpinus betulus were detected in the macrofossil analysis, while the pollen analysis failed to record its presence. The expansion of Carpinus betulus and Picea abies was characteristic of the Subboreal chronozone when both taxa presented antagonistic optima. Alone in north-eastern Poland, there was a re-expansion of deciduous forest in the younger part of the Subboreal chronozone caused probably by low human impact, which is reflected in the whole profile. The first probable traces of human activity were noticed in the Atlantic chronozone and attributed to peoples of the Mesolithic or Early Neolithic cultures, while the first evidence of cultivation was correlated to the Bronze Age. However, the low resolution of the radiocarbon dates did not allow a more precise reconstruction of the chronology. The analysis of macrofossils and tissues indicated two episodes of oligotrophication of the mire. The first one took place during the Boreal chronozone, while the second fall in trophy was triggered by spruce expansion in the Subboreal chronozone. On the other hand, a rise in human impact during the first Carpinus betulus maximum caused eutrophication of the mire.

Large pollen at high temperature: an adaptation to increased competition on the stigma?

Pollen availability is a major constraint of plant reproductive success. Because pollen size trades-off with the quantity of produced grains, the link between climate characteristics and the determination of pollen size is of fundamental importance. To minimize the rate of water loss due to desiccation, a plant should produce larger grains that also have a lower surface-to-volume ratio. We used a comparative analysis to examine the hypothesis predicting increase in pollen size as a response to desiccation intensity. To test the hypothesis, we correlated the data on pollen size with the climate characteristics, temperature and desiccation intensity of the flowering period, for 232 plant species of 11 taxonomic groups. The analysis showed a positive relationship between the pollen size and temperature, but not with the desiccation intensity. We discuss the potential mechanisms by which increased temperature is an indicator of high competition among pollen grains on the stigma, which in turn is expected to promote large pollen. Our work provides insight into the temperature dependence of pollen production in plants and reveals a link between environmental temperature and the intensity of limitation of plant reproductive success by pollen availability. The result is relevant in the context of global climate change. We also discuss why environmental temperature has to be controlled in studies dealing with pollen production, particularly in investigations of size-number trade-off.