Monika Mętrak

The diversity of endophytic fungi in the above-ground tissue of two Lycopodium species in Poland

Endophytes are a large and diverse group of fungi that colonize healthy plant tissues without causing any symptoms. The majority of studies have focused on angiosperm and conifer hosts and few have examined the endophytes of lycophytes. In the present study, we characterized culturable endophytic fungi in two closely related Lycopodium species (L. annotinum and L. clavatum) from pine, beech, oak and spruce forests across Poland. More than 400 strains were isolated but only 18 Ascomycete species were identified. Members of the Dothideomycetes dominated the fungal endophyte communities in Lycopodium. The most abundant taxa cultured were Phoma brasiliensis (from L. clavatum) and Paraconiothyrium lycopodinum (from L. annotinum). Five taxa were isolated exclusively from L. annotinum, but only two of them (Paraconiothyrium lycopodinum and Mycosphaerella sp.) were relatively abundant. Two taxa were only found in L. clavatum, namely: Stagonospora pseudovitensis and an unidentified Dothideomycete. The taxon assigned as Ascomycota 2 (SH219457.06FU) was isolated only from strobili of both host species. Direct PCR and cloning from L. annotinum shoots revealed a substantially greater endophyte richness compared with the results from culturing.

Interspecific differences in foliar 1 PAHs load between Scots pine, birch, and wild rosemary from three polish peat bogs

Pine needles are one of the most commonly used bioindicators of polycyclic aromatic hydrocarbons (PAHs) in the environment. Therefore, the main objective of the current research was the assessment of PAHs accumulation potential of Scots pine (Pinus sylvestris L.) needles in comparison to wild rosemary (Rhododendron tomentosum Harmaja) and birch (Betula spp.) leaves. Our study was carried out on three peat bogs subjected to different degree of anthropopression, which gave us also the opportunity to identify local emission sources. Pine needles had the lowest accumulation potential from all the studied species. The highest accumulation potential, and hence carcinogenic potential, was observed for wild rosemary leaves. As far as emission sources are concerned, the most pronounced influence on atmospheric PAHs loads had traditional charcoal production, resulting in great influx of heavy PAHs. Observed seasonal changes in PAHs concentrations followed the pattern of winter increase, caused mainly by heating season, and summer decrease, caused mainly by volatilization of light PAHs.

Does the Function of Railway Infrastructure Determine Qualitative and Quantitative Composition of Contaminants (PAHs, Heavy Metals) in Soil and Plant Biomass?

Polycyclic aromatic hydrocarbons (PAHs) and heavy metals are the two most important groups of pollutants associated with rail transport. Both have a serious negative impact on the natural environment, including human health and degradation of sensitive ecosystems. In our study, apart from qualitative and quantitative analysis of the main PAHs and heavy metals associated with rail, we tried to assess composition of specific compounds related to functional areas of railway infrastructure and to distinguish potential chemical markers which can be used for identification of pollution. Moreover, we evaluated the applicability of plants overgrowing railway infrastructure as bioindicators of rail-associated pollution. Though we confirmed that high amounts of PAHs and heavy metals in soil are characteristic for intensively used railway infrastructure, we found no typical pollution profiles for the differently used areas (i.e. platforms, sidings, cleaning bays). The major source of these contaminants is petroleum products used in conservation of railway infrastructure and rolling stock. As far as the use of plants overgrowing railway infrastructure as bioindicators of rail-associated pollution is concerned, it is rather limited, due to frequent application of herbicides for security and track stability reasons.

Long-term fire effects of the drained open fen on organic soils

Abstract Fire has considerable impact on vegetation and organic soils properties. As we observed that the differences between vegetation of burnt and unburnt areas on the rich fen are visible 11 years after the fire, we assumed that the post-fire changes are long lasting, yet limited exclusively to the burnt areas. In order to check this hypothesis we studied spatial differentiation of physical and chemical properties of soils, and productivity capacities of burnt and unburnt areas in the fen in Biebrza National Park. We took soil samples from the neighboring burnt and unburnt areas, from the depth of 0–30 cm and 30–50 cm. We analyzed 21 parameters of the soils including: pH, ash content, moisture, bulk density, exchangeable K, Na, Ca, available P, N-NH4+, N-NO3−, total N, C, K, Na, Ca, Mg, Fe, P; and calculated C:N, C:P ratios. Surface layer of the burnt soils differed significantly from the unburnt soils in respect of 17 out of 21 parameters. The most pronounced difference was observed for available phosphorous (on average 6 times higher for the burnt soils). The differences in the deeper layer were mostly insignificant. The burnt areas were also characterized by twofold higher plant productivity than recorded for the unburnt areas. The influence of fire on peaty soils was long lasting but mostly limited to the surface layer of the soils. In the case of particular soil features, the post-fire differences were modified by advanced muck formation (moorshing) processes in the unburnt areas. Since the fire led to long lasting increase of fertility, the recovery of fen vegetation is unlikely.

Paleoclimatological and Geoarchaeological Significance of the Holocene Loess – Soil Successions of the Tien Shan Foothills of Uzbekistan

Abstract Scenario of climate changes in the Late Pleistocene and Holocene in Central Asia is very fragmentary and inconsistent. Therefore, interaction between the development and decline of ancient cultures and the climate fluctuations are difficult to be traced. To resolve this problem, the key role can be played by multidisciplinary studies of unique Late Pleistocene and especially Holocene loess – soil succession of the Tien Shan foothills in Uzbekistan. This area yields unique successions of paleosols interbedded with loesses that are particularly useful for paleoclimate analysis. They are represented by continuous and uninterrupted sedimentary sequences with a highly varied record of magnetic susceptibility. As such, they contain a full sequence of short-term climatic oscillations of the Holocene in high resolution and therefore, in this case, they are exceptional on global scale. The correlation of Late Pleistocene and Holocene climate changes scenario with stages of development and collapse of the past human settlement in Central Asia seems at present one of the most promising research directions, in line with the substantial course of interdisciplinary research on the interaction between humans and the natural environment.

Creeping Environmental Problems in the Pamir Mountains: Landscape Conditions, Climate Change, Wise Use and Threats

Pamir is a highland region in Central Asia, located on the orogenic uplift known as the Pamir Knot which joins several Asian mountain ranges. The name Pamir may derive from the ancient Iranian pai-mir, “foot of Mithra,” god of the sun or from the word pamers, which means the flat and wide high valleys with typical mountain meadows in altitudes around 3500–4000 m above sea level (a.s.l.) High mountain topography (the highest ranges exceed 7000 m a.s.l.) is a characteristic feature of this region and acts as a barrier isolating it from the rest of the world.

Age and land use as factors differentiating hydrochemistry and plant cover of astatic ponds in post-agricultural landscape

Abstract Small, astatic ponds are important features of post-glacial landscape, which support heterogeneity and biodiversity of agricultural areas. In the presented research we explored differences in hydrochemistry and plant cover of 20 small ponds located in Northeastern Poland, characterized by diverse age and developed in differently managed areas. According to our research, though changes in water level are under direct influence of water balance in the catchment, to which belonged the ponds, their hydrochemistry seemed to be shaped by processes at the level lower than the catchment scale. Age of the ponds appeared to be an important factor influencing density and species composition of vegetation developed on the studied ponds.

Nature's patchwork: How water sources and soil salinity determine the distribution and structure of halophytic plant communities in arid environments of the Eastern Pamir

The eastern part of the Pamir Mountains, located in Central Asia, is characterized by great climatic continentality and aridity. Wetlands developed in this hostile region are restricted to spring areas, terraces of shallow lakes or floodplains along rivers, and provide diversified ecosystem services e.g. as water reservoirs, refugia for rare species and pastures for domestic cattle. These ecosystems are particularly susceptible to climate changes, that in the Pamir Mountains result in increased temperatures, intense permafrost/glacial melt and alterations of precipitation patterns. Climatic changes affect pasture management in the mountains, causing overutilization of sites located at lower elevations. Thus, both climate and man-induced disturbances may violate the existing ecological equilibrium in high-mountain wetlands of the Eastern Pamir, posing a serious risk to their biodiversity and to food security of the local population. In this context, we sought to assess how environmental drivers (with special focus on soil features and potential water sources) shape the distribution and diversity of halophytic plant communities developed in valleys in the Eastern Pamir. This task was completed by means of a vegetation survey and comprehensive analyses of habitat conditions. The lake terraces and floodplains studied were covered by a repetitive mosaic of plant communities determined by differences in soil moisture and salinity. On lower, wetter sites, this patchwork was formed by Blysmus rufus dominated salt marshes, saline small sedge meadows and saline meadows with Kobresia royleana and Primula pamirica; and on drier, elevated sites, by endemic grasslands with Hordeum brevisubulatum and Puccinellia species and patches of xerohalophytic vegetation. Continuous instability of water sources and summer droughts occurring in the Pamir Mountains may lead to significant structural and functional transformations of described wetland ecosystems. Species more tolerant to decreased soil moisture and/or increased soil salinity will expand, leading to alterations of ecosystem services provided by the Pamirs’ wetlands. The described research will help to assess the current state of the wetlands and to predict directions of their future changes.

From Deserts to Glaciers: Magnetometry in Paleoenvironmental Studies in Central Asia

Central Asia is a vast area extending from the Caspian Sea to the western borders of China, with highly differentiated geography (high mountains, excessive deserts, grassy steppes), geology and anthropological history. As such it poses a perfect site for various studies, including paleoenvironmental and paleoclimatic research. Up to now, we performed two distinct studies in Central Asia: (1) a preliminary study on the Holocene loess-soil succession in the Karasu Valley, at the Tien Shan foothills in Uzbekistan; (2) and a study on development of high mountain lakes in the Pamir, based on lake sediments of the Rangkul Lake in the Eastern Pamir, Tajikistan (initial results presented here). In both studies we used magnetic properties of studied sediments and soils as one of parameters in multiproxy analyses. Paleosols from the Karasu Valley developed into continuous and uninterrupted sedimentary sequences interbedded with loess horizons. These sequences are characterized by diversified magnetic susceptibility values that reflect changes in their formation. Sediments form the Rangkul Lake show 5 significant shifts in deposition processes, caused by changes in water level. These shifts are clearly reflected both in sediment composition and in magnetic susceptibility of deposited material.