Monique Guis
École Normale Supérieure
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Featured researches published by Monique Guis.
Plant Cell Reports | 1998
Mohamed Ben Amor; Monique Guis; Alain Latché; Mondher Bouzayen; Jean-Claude Pech; Jean-Paul Roustan
Abstract The role of ethylene in shoot regeneration was investigated using transgenic Cucumis melo plants expressing an antisense 1-aminocyclopropane-1-carboxylate (ACC) oxidase gene. ACC oxidase catalyses the last step of ethylene biosynthesis. Leaf and cotyledon explants from the transgenic plants exhibited low ACC oxidase activity and ethylene production, whereas the regeneration capacity of the tissues was greatly enhanced (3.5- and 2.8-fold, respectively) compared to untransformed control tissues. Addition of ethylene released by 50 or 100 µm 2-chloroethylphosphonic acid dramatically reduced the shoot regeneration rate of the transgenic tissues. The results clearly demonstrate that ethylene plays an important role in C. melo morphogenesis in vitro.
Scientia Horticulturae | 1997
Monique Guis; Alain Latché; Jean-Claude Pech; Jean-Paul Roustan
Abstract This paper describes a system of regeneration that allows somatic embryo formation at high frequency from mature cotyledon explants of Cucumis melo L. var. cantalupensis (cv. Vedrantais). Several different growth factors, environmental conditions and carbohydrate sources were analysed in order to improve somatic embryogenesis in this cultivar. Best conditions lead to the definition of a two step protocol including a 2 week period of embryogenesis induction in Murashige and Skoogs medium supplemented with 30 g l −1 glucose, 10 μM 2,4-dichlorophenoxyacetic acid and 0.5 μM 6-benzylaminopurine (IM medium) in the darkness, followed by a period of embryo development on the same medium without hormone (DM medium), under a 16/8 h light/dark photoperiod. After 4 weeks of culture, somatic embryos were converted into plantlets on the DM medium in a Magenta vessel. Using this optimized protocol, 42% of the explants were able to generate embryos with an average of 7.8 embryos per explant. More than 60% of the plants generated from these embryos were diploid, exhibited the phenotype expected and gave well-developed fruit.
Archive | 1999
Monique Guis; M. Ben Amor; R. Botondi; Ricardo Antonio Ayub; Alain Latche; Mondher Bouzayen; Jean-Claude Pech
The melon, Cucumis melo L. is an ancient plant, likely originating from the tropical Africa/Middle-East regions. It then spread out to the Mediterranean, India and Asia (Withaker & Davis, 1962) from where a large diversity of fruit types have now emerged (Kirkbride, 1993). Within the Cucumis melo species, a wide range of ripening behaviour exists. For example, some varieties tend to have a sharp climacteric respiration associated with a fast fruit ripening and abscission rate (Lyons et al., 1962). In contrast, other varieties do not abscise at maturity, and the climacteric may be absent (Nukaya et al., 1986) or extended over several days (Pratt et al., 1977).
Nature Biotechnology | 1996
Ricardo Antonio Ayub; Monique Guis; Mohamed Ben Amor; Laurent Gillot; Jean-Paul Roustan; Alain Latché; Mondher Bouzayen; Jean-Claude Pech
Journal of Experimental Botany | 2007
Kiyomi Nishiyama; Monique Guis; Jocelyn K. C. Rose; Yasutaka Kubo; Kristen A. Bennett; Lu Wangjin; Kenji Kato; Koichiro Ushijima; Ryohei Nakano; Akitsugu Inaba; Mondher Bouzayen; Alain Latché; Jean-Claude Pech; Alan B. Bennett
Journal of The American Society for Horticultural Science | 1997
Monique Guis; Rinaldo Botondi; Mohamed Ben-Amor; Ricardo Antonio Ayub; Mondher Bouzayen; Jean-Claude Pech; Alain Latché
Plant Physiology | 2000
Kristen A. Hadfield; Tam Dang; Monique Guis; Jean-Claude Pech; Mondher Bouzayen; Alan B. Bennett
Scientia Horticulturae | 2000
Monique Guis; Mohamed Ben Amor; Alain Latché; Jean-Claude Pech; Jean-Paul Roustan
Plant Physiology | 1999
Ségolène Colrat; Alain Latché; Monique Guis; Jean-Claude Pech; Mondher Bouzayen; Jean Fallot; Jean-Paul Roustan
Plant Journal | 1998
Pedro Guillen; Monique Guis; Gracia Martinez-Reina; Ségolène Colrat; Sylvie Dalmayrac; Corine Deswarte; Mondher Bouzayen; Jean-Paul Roustan; Jean Fallot; Jean-Claude Pech; Alain Latché