Monnipha Sila-asna
Mahidol University
Network
Latest external collaboration on country level. Dive into details by clicking on the dots.
Publication
Featured researches published by Monnipha Sila-asna.
Development Growth & Differentiation | 2006
Shutipen Buranasinsup; Monnipha Sila-asna; Narong Bunyaratvej; Ahnond Bunyaratvej
Embryonic tissue and organ development are initiated from three embryonic germ layers: ectoderm (skin and neuron), mesoderm (blood, bone, muscle, cartilage and fat) and endoderm (respiratory and digestive tract). In former times, it was believed that cell types in each germ layer are specific and do not cross from one to another throughout life. A new finding is that one tissue lineage can differentiate across to another tissue lineage, and this is termed transdifferentiation. We were interested in studying the transdifferentiation of skin‐derived precursor cells (ectoderm layer) to osteoblastic cells (mesoderm layer). Human skin‐derived precursor cells (hSKP) were isolated and induced into an osteoblastic lineage using osteogenic induction medium (α‐MEM plus 10% fetal bovine serum supplemented with ascorbic acid, β‐glycerophosphate and dexamethasone). The specific characteristics of osteoblastic cells, including the expression of enzyme alkaline phosphatase, the deposition of mineral and the expression of osterix, bone sialoprotein and osteocalcin, were detected only from the inductive group. The results in our study show that SKP from human skin are a practically available source for osteogenesis. The samples are easily obtainable for autologous use with a high expansion capacity.
Biochemical and Biophysical Research Communications | 2012
Kriengsak Lirdprapamongkol; Khajeelak Chiablaem; Monnipha Sila-asna; Rudee Surarit; Ahnond Bunyaratvej; Jisnuson Svasti
Vasculogenic mimicry (VM) is the phenomenon where cancer cells mimic endothelial cells by forming blood vessels. A stem cell-like phenotype has been proposed to be involved in this tumor plasticity. VM seems to correlate with metastasis rate, but there have been no reports on the effects of pro-metastatic and pro-angiogenic factors or hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) on VM formation of hepatocellular carcinoma (HCC) cells. Here, we determine VM capacity and expression of stemness genes (Oct4, Sox2, Nanog and CD133) in well- and poorly-differentiated HCC cell lines. The poorly-differentiated cell line SK-Hep-1 with mesenchymal features (high invasiveness and expressing Vimentin, with no E-cadherin) could form VM in vitro, while the well-differentiated cell line HepG2 did not form VM. There was no correlation between expression of stemness genes and intrinsic VM capacity. However, HGF but not VEGF, could induce VM formation in HepG2, concomitant with epithelial-mesenchymal transition (EMT), de-differentiation and increased expression of stemness genes. Our results show that the role of stemness genes in VM capacity of HCC cells is likely to depend on differentiation status.
International Journal of Ophthalmology | 2012
Sarawut Saichanma; Ahnond Bunyaratvej; Monnipha Sila-asna
The damage of human corneal cells encounter with the problem of availability of corneal cells for replacement. Limitation of the source of corneal cells has been realized. An attempt of development of corneal epithelial-like cells from the human skin-derived precursor (hSKPs) has been made in this study. Combination of three essential growth factors: epidermal growth factor (EGF), keratinocyte growth factor (KGF) and hepatocyte growth factor (HGF) could demonstrate successfully induction of hSKPs to differentiation into corneal cells.The induced cells expressed the appearance of markers of corneal epithelial cells as shown by the presence of keratin 3 (K3) by antibody label and Western blot assay. The K3 gene expression of induced hSKPs cells as shown by reverse transcription-polymerase chain reaction (RT-PCR) technology was also demonstrated. The presence of these markers at both gene and protein levels could lead to our conclusion that the directional transdifferentiation of hSKPs cells into corneal epithelial cells was successfully done under this cell induction protocol. The finding shows a newly available stem cell source can be obtained from easily available skin. Cells from autologous human skin might be used for corneal disorder treatment in future clinical application.
The Kobe journal of the medical sciences | 2007
Monnipha Sila-asna; Ahnond Bunyaratvej; Sakan Maeda; Hiromichi Kitaguchi; Narong Bunyaratavej
Asian Biomedicine | 2010
Narisa Futrakul; Monnipha Sila-asna; Prasit Futrakul
in Vivo | 2008
Kriengsak Lirdprapamongkol; Jan-Peter Kramb; Daranee Chokchaichamnankit; Chantragan Srisomsap; Rudee Surarit; Monnipha Sila-asna; Ahnond Bunyaratvej; Gerd Dannhardt; Jisnuson Svasti
Renal Failure | 2006
Monnipha Sila-asna; Ahnond Bunyaratvej; Prasit Futrakul; Narisa Futrakul
Southeast Asian Journal of Tropical Medicine and Public Health | 2005
Yaowaree Kittikalayawong; Monnipha Sila-asna; Ahnond Bunyaratvej
Clinical Hemorheology and Microcirculation | 2007
Narisa Futrakul; Monnipha Sila-asna
Journal of the Medical Association of Thailand Chotmaihet thangphaet | 2011
Katesaree Suriyachand; Ahnond Bunyaratvej; Narong Bunyaratavej; Monnipha Sila-asna