Monroe K. Coleman

Isolation of C-reactive proteins of man, monkey, rabbit and dog by affinity chromatography on phosphorylated cellulose.

Abstract The ability of C-reactive protein (CRP) to complex with organic monophosphates in the presence of calcium was exploited in devising a method for isolating this protein in a simple, highly selective way employing phosphorylated cellulose. The same procedure was found to provide CRPs from monkey, rabbit and dog as well and may prove to be a general procedure for the isolation of CRP of other species.

CX-REACTIVE PROTEIN RESPONSES IN THE RABBIT AFTER WHOLE-BODY IRRADIATION

Cx-protein was present in the serum of all rabbits exposed to whole-body doses of 250 to 750 r of x rays 24 hours after exposure, and the maximum Cx- protein titer was usually observed at this time. Not all animals gave a Cx- protein titer 24 hours after 100 r or less. The mean 24-hour titer was about the same after doses of 100 to 750 r. The response was commonly biphasic in fed rabbits and monophasic in rabbits denied food during the postirradiation period. Treatment with compound 48/80 produced a Cx-protein response and animals so treated behaved like controls when subsequently given 250 r. Pyrilamine did not modify the Cx-protein response to 250 r of x rays. (auth)

Behavior of the c-reactive protein during purification and electrophoretic characterization☆

Abstract C-reactive protein prepared chromatographically on DEAE cellulose is obtained in part as an aggregated complex with the mobility in 7 1 2 % polyacrylamide typical of 7 S γ-globulin. The aggregated protein slowly dissociates on chromatography and on electrophoresis in polyacrylamide gel to give components antigenically related to C-reactive protein with electrophoretic mobilities in polyacrylamide similar to those of pre and post albumins. This is also true for C-reactive protein prepared by the conventional method. The electrophoretic mobility of C-reactive protein in polyacrylamide depends on the preparative history and age of the sample. The relative mobilities of its subunits were independent of method of isolation, but differed in their mobility from one individual to another, suggesting the existence of multiple molecular forms of this protein. The polyacrylamide matrix in which proteins have been resolved electrophoretically can be conveniently adapted to provide immunoelectrophoretic data.

Effect of drugs on Cx-protein responses in the rabbit.

Summary Methyl prednisolone and fluorometholone prevented the appearance of CxRP in rabbit serum following subcutaneous injection of mineral oil-Aquaphor emulsion. Fluorometholone was unable to block CxRP responses to 250 r whole body irradiation or to intravenous injection of Thorotrast or Varidase. It did block CxRP responses to intravenous injection of post irradiation seromucoid or TMV and the intravascular formation of antigen-antibody complexes. Under appropriate time dose conditions fluorometholone inhibited both antibody production and CxRP appearance while under other time dose conditions CxRP appearance was prevented without alteration of antibody production.