Monte A. Mayes

Two New Species of Acanthobothrium Van Beneden 1849 (Cestoidea: Tetraphyllidea) from Freshwater Stingrays in South America

Two new species of Acanthobothrium are reported from freshwater stingrays in northern South America. Acanthobothrium quinonesi sp. n. is described from Potamotrygon magdalenae Dumeril collected from the Magdalena River in northern Colombia and A. amazonensis sp. n. from P. circularis Garman collected in the Itacuai River of northwestern Brazil. Acanthobothrium quinonesi differs from A. amazonensis by having a smaller and anteriorly-curved cirrus sac, 43-60 rather than 50-72 testes, and bothridial hooks 100--142 ,urn long rather than 145-184 ,urn long. Characters used in the taxonomy of Acanthobothrium species are listed to show that the new species resemble each other and A. terezae, also from a South American freshwater stingray, more than they do any other members of the genus. Species of Acanthobothrium van Beneden 1849 parasitize a variety of marine and estuarine elasmobranchs (Goldstein, 1967; Williams, 1969) . Collections from the freshwater stingrays Potamotrygon magdalenae Dumeril in the Magdalena River of Colombia and from P. circularis Garman in th e Itacuai River of Brazil, contained specimens of two new species of Acanthobothrium which resemble each other more than they do any other known species. Worms were removed from host spiral valves, examined alive and fixed with warm AFA, or fixed in situ with 10% formalin; all were stored in 70 % ethanol. Study included examination of whole mounts stained with Mayers hematoxylin and mounted in Canada balsam as well as study of serial cross sections cut at 8 p.m and stained with hematoxylin-eosin. Measurements are in urn unless otherwise stated with number of measurements (N) and mean values (x) indicated for certain characteristics. Figures were drawn with the aid of a drawing tube. Acanthobothr;um qu;nones; sp. n.

Acanthobothrium electricolum sp. n. and A. lintoni Goldstein, Henson, and Schlicht 1969 (Cestoda: Tetraphyllidea) from Narcine brasiliensis (Olfers) (Chondrichthyes: Torpedinidae) in Colombia

Acanthobothrium lintoni and a new species of Acanthobothrium are reported from the lesser electric ray Narcine brasiliensis in Colombia. The new species resembles all other members of the genus parasitizing torpedinids by having a relatively small scolex, long and spinose cephalic peduncle, fewer than 75 testes per proglottid, an indistinct genital atrium, and V-shaped ovaries with ovarian lobes of equal length which do not reach the posterior margin of the cirrus sac. The new species differs from all known species by having bothridial hooks only 46 to 64 ,um in total length. A key to the species of Acanthobothrium parasitizing torpedinids is presented. Species of tetraphyllidean cestodes belong- ing to the genus Acanthobothrium Beneden 1849 and parasitizing elasmobranchs of the family Torpedinidae possess relatively small scolices, extremely long cephalic peduncles covered with spines, fewer than 75 testes per proglottid, indistinct genital atria, and V- shaped ovaries with equal-length lobes which do not reach anteriorly to the posterior margin of the cirrus sac. Examination of 14 specimens of the lesser electric ray Narcine brasiliensis (Olfers) captured in the vicinity of Cartagena, Colombia, revealed infections of two species of Acanthobothrium: A. lintoni Goldstein, Hen- son, and Schlicht 1969, and a new species described herein. Rays were collected with beach seines and kept in holding tanks until examined. Helminths were removed from the

New species of Gyrodactylus and Dactylogyrus (Trematoda: Monogenea) from fishes of Nebraska.

Six new monogenetic trematodes are described from Nebraska fishes: Gyrodactylus bulbacanthus sp. n. from Fundulus kansae (Garman); G. callawayensis sp. n. from Notropis lutrensis (Baird and Girard); G. eos sp. n. from Phoxinus eos (Cope); G. nebraskensis from Phoxinus neogaeus (Cope); G. planensis from Notropis dorsalis (Agassiz); and Dactylogyrus pectenatus from Pimephales promelas (Rafinesque). This is the second report of monogeneans from Nebraska fishes.

Telorchis gutturosi sp. n. (Trematoda: Telorchiidae) from Graptemys pseudogeographica Gray in Nebraska, with Reports of Additional Species of Trematodes from Nebraska Turtles

One species of digenean is described and 8 others reported from turtles in Nebraska. Telorchis gutturosi, described from Graptemys pseudogeographica, resembles T. stossichi, T. pseudoaculeatus, and T. pleroticus in having an acetabulum significantly smaller than the oral sucker, but differs by possessing a pharynx which is as large as the acetabulum and having the ovary only 11o the body length from the acetabulum. Telorchis necturi is considered the senior synonym of T. cryptobranchi; Graptemys pseudogeographica is a new host. Hapalorhynchus stunkardi is reported for the first time since its descrintion. Based on new morphological evidence, the generic diagnosis of Hapalorhynchus is emended and a key to the species provided. Spirorchis scripta, S. parvus, Protenes angustus, and Eustomos chelydrae are reported for the first time from Nebraska. Graptemys pseudogeographica is a new host for Heronimus mollis; Kinosternon flavescens is a new host for Telorchis corti. Twenty-one species of platyhelminth parasites have been reported from Nebraska turtles (Brooks and Mayes, 1975). The present study reports seven additional species, one of which is described as new, and new hosts for two previously reported species. Worms were removed from the host, flattened with slight coverslip pressure or not flattened (spirorchiids), fixed with AFA, stored in 70% ethanol, stained with Mayers hematoxylin or Mayers carmalum, and mounted in Canada balsam for study as whole mounts. Measurements are in micrometers unless otherwise stated; figures were drawn with the aid

Phyllodistomum scrippsi sp. n. (Digenea: Gorgoderidae) and Neobenedenia girellae (Hargis, 1955) Yamaguti, 1963 (Monogenea: Capsalidae) from the California Sheephead, Pimelometopon pulchrum (Ayres) (Pisces: Labridae)

Phyllodistomum scrippsi sp. n. is described from the urinary bladder and Neobenedenia girellae is reported from the skin of Pimelometopon pulchrum from La Jolla, California. Phyllodistomum scrippsi differs from P. borisbychowskyi in possessing lobed vitellaria, fewer extracecal uterine loops, irregular to lobed gonads, and larger eggs; and from P. acceptum in possessing a short esophagus, nondigitiform vitelline lobes, and an oral sucker larger than the acetabulum. An annotated checklist of trematodes parasitizing P. pulchrum is given. A specimen of the California sheephead, Pimelometopon pulchrum (Ayres), was collected off La Jolla, California, in March 1974. The urinary bladder contained eight specimens of an unreported species of Phyllodistomum Braun, 1899 (Digenea: Gorgoderidae), and over 100 specimens of the monogenean Neobenedenia girellae (Hargis, 1955) Yamaguti, 1963, were found on the skin. The digeneans were fixed in situ with hot AFA, removed from the urinary bladder, and stored in 70% ethanol. Monogeneans were collected as suggested by Rogers (1966) and stored in 10% formalin. All worms were stained with Mayers hematoxylin and mounted in Canada balsam for study as whole mounts. Figures were drawn with the aid of a camera lucida. All measurements are in microns unless