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Featured researches published by Mouna Hassine.


Journal of Clinical Microbiology | 2008

Acute Infantile Gastroenteritis Associated with Human Enteric Viruses in Tunisia

Khira Sdiri-Loulizi; Hakima Gharbi-Khelifi; Alexis de Rougemont; Slaheddine Chouchane; Nabil Sakly; Katia Ambert-Balay; Mouna Hassine; Mohamed Neji Guediche; Mahjoub Aouni; P. Pothier

ABSTRACT This prospective study, conducted from January 2003 to June 2005, investigated the incidence and the clinical role of various enteric viruses responsible for infantile gastroenteritis in 632 Tunisian children presenting in dispensaries (380 children) or hospitalized (252 children) for acute diarrhea. At least one enteric virus was found in each of 276 samples (43.7%). A single pathogen was observed in 234 samples, and mixed infections were found in 42 samples. In terms of frequency, rotavirus and norovirus were detected in 22.5 and 17.4% of the samples, respectively, followed by astrovirus (4.1%), Aichi virus (3.5%), adenovirus types 40 and 41 (2.7%), and sapovirus (1.0%). The seasonal distribution of viral gastroenteritis showed a winter peak but also an unusual peak from May to September. The severity of the diarrhea was evaluated for hospitalized infants. No significant differences were observed between rotavirus and norovirus infections with regard to the incidence and the clinical severity of the disease, especially in dehydration.


Journal of Clinical Microbiology | 2009

Molecular Epidemiology of Norovirus Gastroenteritis Investigated Using Samples Collected from Children in Tunisia during a Four-Year Period: Detection of the Norovirus Variant GGII.4 Hunter as Early as January 2003

Khira Sdiri-Loulizi; Katia Ambert-Balay; Hakima Gharbi-Khelifi; Nabil Sakly; Mouna Hassine; Slaheddine Chouchane; Mohamed Neji Guediche; Pierre Pothier; Mahjoub Aouni

ABSTRACT Human noroviruses (NoVs) cause epidemic and endemic acute gastroenteritis in children and adults. To study the prevalence and genetic diversity of NoV in children in Tunisia, a total of 788 fecal samples were collected during a 4-year period in the region of Monastir, from children 12 years of age or younger, hospitalized or presenting in dispensaries with symptoms of acute gastroenteritis. NoV was detected by reverse transcription-PCR and confirmed by sequence analysis. This is the first report that describes the molecular epidemiology of NoV in Tunisian children: NoVs were characterized as the causative agent in 128 (16.2%) of the samples. Fourteen samples contained a mixture of two NoVs, and 33 samples were coinfected with additional enteric viruses. Eight distinct NoV genotypes were detected (GGI.2, GGI.4, GGII.1, GGII.4, GGII.8, GGII.14, GGIIb/GGII.2, and GGIIb/GGII.3). GGII.4 was the most prevalent genotype, accounting for 83 (64.8%) cases. Interestingly the GGII.4 variant Hunter, described as spreading all over the world in 2004, was found in Tunisia as early as January 2003. The delay of 1 year between the isolation in Tunisia and the worldwide emergence is somewhat surprising, considering the importance of the contacts between North Africa and Europe particularly. Nevertheless, this illustrates the idea that sporadic gastroenteritis cases may be a reservoir for emerging epidemic NoV strains.


Journal of Clinical Microbiology | 2009

Detection and Genomic Characterization of Aichi Viruses in Stool Samples from Children in Monastir, Tunisia

Khira Sdiri-Loulizi; Mouna Hassine; Hakima Gharbi-Khelifi; Nabil Sakly; Slaheddine Chouchane; Mohamed Neji Guediche; Pierre Pothier; Mahjoub Aouni; Katia Ambert-Balay

ABSTRACT Aichi virus has been associated with acute gastroenteritis in adults and children. Stool samples were collected from 788 Tunisian children suffering from diarrhea. Aichi virus was found in 4.1% of the cases. The high proportion of monoinfections and the high frequency of hospitalizations support the role of Aichi virus in pediatric gastroenteritis.


Journal of Medical Virology | 2009

Molecular epidemiology of human astrovirus and adenovirus serotypes 40/41 strains related to acute diarrhea in Tunisian children.

Khira Sdiri-Loulizi; Hakima Gharbi-Khelifi; Alexis de Rougemont; Mouna Hassine; Slaheddine Chouchane; Nabil Sakly; P. Pothier; Mohamed Neji Guediche; Mahjoub Aouni; Katia Ambert-Balay

Human astrovirus (AstV) and adenovirus types 40 and 41 (AdV 40/41) are responsible for epidemic and endemic acute gastroenteritis in children and adults. The present study was designed to evaluate the prevalence and genetic diversity of enteric viruses in children in Tunisia. A total of 788 fecal samples were collected during a 4‐year period in the region of Monastir, from children under 12 years old, hospitalized or presenting in dispensaries with symptoms of acute gastroenteritis. AstV and AdV40/41 were detected by immunoenzymatic methods and confirmed by PCR/RT‐PCR and sequence analysis. Phylogenetic analyses were performed for nucleotide homology with reference strains. AstV and AdV40/41 were characterized as a causative agent in 28 (3.6%) and 18 (2.3%) of the fecal samples, respectively. Phylogenetic analysis showed that the AstVs belonged to the serotypes 3 (n = 4; 14.3%) and 1 (n = 24; 85.7%), and the enteric AdVs to the serotypes 40 (n = 1; 5.6%) and 41 (n = 17; 94.4%). This is the first report that describes the molecular epidemiology of AstV and AdV40/41 in Tunisian children. Their respective detection rate was very low, far below that of rotavirus and norovirus. The genetic diversity among these two viruses is relatively limited and varies depending on the area. J. Med. Virol. 81:1895–1902, 2009.


Clinical and Vaccine Immunology | 2010

Aichi Virus IgG Seroprevalence in Tunisia Parallels Genomic Detection and Clinical Presentation in Children with Gastroenteritis

Khira Sdiri-Loulizi; Mouna Hassine; Jean-Baptiste Bour; Katia Ambert-Balay; Maha Mastouri; Ludwig-Serge Aho; Hakima Gharbi-Khelifi; Zaidoun Aouni; Nabil Sakly; Slaheddine Chouchane; Mohamed Neji-Guédiche; Pierre Pothier; Mahjoub Aouni

ABSTRACT Aichi virus has been described as a novel causative agent of gastroenteritis in humans. In this study, we report the seroprevalence distribution of Aichi virus in Tunisia. A panel of 1,000 sera was screened by applying an enzyme-linked immunosorbent assay for immunoglobulin G specific for Aichi virus. A considerable prevalence (92%) of antibody to Aichi virus was found across all age groups. The specific anti-Aichi virus antibodies increased with age, from a high rate (68.8%) in children under 10 years old to about 100% in persons more than 60 years old. We found a statistically significant increase in levels of antibody to Aichi virus according to the age of patients. Immunoglobulin M antibodies were detected among five children. A high frequency of Aichi virus monoinfections in hospitalized children with severe gastroenteritis was previously observed in Tunisia. Aichi virus causes diarrhea with dehydration, fever, and vomiting. This work is the first to establish a correlation between the high seroprevalence of specific Aichi virus antibodies, clinical presentation, and a high frequency of isolation of Aichi virus by genomic characterization in stools of children suffering from gastroenteritis. Our data show the importance and emerging character of Aichi virus in the viral etiology of pediatric gastroenteritis.


Canadian Journal of Microbiology | 2011

Molecular epidemiology and clinical characterization of group A rotavirus infections in Tunisian children with acute gastroenteritis

Khira Sdiri-Loulizi; Katia Ambert-Balay; Hakima Gharbi-Khelifi; Mouna Hassine; Slaheddine Chouchane; Nabil Sakly; Mohamed Neji-Guédiche; Pierre Pothier; Mahjoub Aouni

Rotaviruses are the most common cause of severe viral gastroenteritis in early childhood worldwide. Thus, the objectives of our study were to determine the molecular epidemiology and the clinical features of rotavirus gastroenteritis in Tunisia. Between January 2003 and April 2007, a prospective study was conducted on 788 stool samples collected from children under 12 years of age who were suffering from acute gastroenteritis. Rotavirus was detected by multiplex RT-PCR in 27% (n = 213) of samples, among them 79.3% (n = 169) cases were monoinfections. The frequency of rotavirus infections was significantly higher among inpatients (29%) than among outpatients (13%) (P < 0.001). The seasonal distribution of rotavirus diarrhea showed a winter peak, with an unusual peak from June to September. The mean duration of hospitalization was 6.5 ± 8.1 days and the mean age was 15.8 ± 22.8 months for rotavirus monoinfections. Fever, vomiting, abdominal pain, and dehydration were observed in 88, 98, 13, and 80 cases, respectively, in children with rotavirus monoinfections. G3P[8] (45.6%) and G1P[8] (23.9%) were the most common genotypes found in our study. The determination of rotavirus infection prevalence and the characterization of the rotavirus strains circulating will help us to better understand the molecular biology and epidemiology of the disease in our country.


Case reports in nephrology | 2011

Cerebral Sinovenous Thrombosis in a Child with Idiopathic Nephrotic Syndrome

L. Ghédira Besbès; S. Haddad; A. Gabsi; Mouna Hassine; Ch. Ben Meriem; M.-N. Guediche

Nephrotic syndrome (NS) is a renal disorder characterized by heavy proteinuria, hypoalbuninemia, edema and hypercholesterolemia. Nephrotic syndrome in children is known to be associated with an hypercoagulable state and thromboembolic complications. However cerebral sinovenous thrombosis (CSVT) is very rare. Here we report a seven-year-old child with steroid-dependent idopathic nephrotic syndrome resulting from a minimal change disease, developed multiple cerebral sinovenous thrombosis, presenting with headache, left sixth nerve palsy, and papilledema. The diagnosis of CSVT was established by cranial computed tomography, magnetic resonance imaging, and magnetic resonance angiography. He gradually recovered after anticoagulant therapy. CSVT is very rare in nephrotic children. The diagnosis of CSVT should be considered in any patient with nephrotic syndrome who develops neurologic symptoms. This report highlights the importance of suspecting and recognizing this potentially life threatening complication and initiating early treatment.


BMC Research Notes | 2011

Bacteriological and physico-chemical assessment of wastewater in different region of Tunisia: impact on human health

Imen Ben Salem; Imen Ouardani; Mouna Hassine; Mahjoub Aouni


Virus Genes | 2011

Molecular detection of genogroup I sapovirus in Tunisian children suffering from acute gastroenteritis

Khira Sdiri-Loulizi; Mouna Hassine; Hakima Gharbi-Khelifi; Zaidoun Aouni; Slaheddine Chouchane; Nabil Sakly; Mohamed Neji-Guédiche; Pierre Pothier; Katia Ambert-Balay; Mahjoub Aouni


La Tunisie médicale | 2010

Détection des virus entériques dans les eaux usées de la région de monastir par RT-PCR.

Mouna Hassine; Khira Sdiri; Samira Riabi; Abir Beji; Zaidoun Aouni; Mahjoub Aouni

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Khira Sdiri

University of Monastir

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S. Hadded

University of Monastir

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Sonia Hamdi

University of Monastir

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Walid Jomaa

University of Monastir

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