Muammer Goncuoglu

Serotype distribution of Salmonella isolates from turkey ground meat and meat parts.

The aim of the study was to find out the serotype distribution of 169 Salmonella colonies recovered from 112 Salmonella positive ground turkey (115 colonies) and 52 turkey meat parts (54 colonies). Out of 15 Salmonella serotypes: S. Corvallis, S. Kentucky, S. Bredeney, S. Virchow, S. Saintpaul and S. Agona were identified as the predominant serovars at the rates of 27%, 13%, 12%, 12%, 11%, and 10%, respectively. Other serotypes were below 6% of the total isolates. All S. Kentucky and S. Virchow and most of the S. Corvallis (39/46) and S. Heidelberg (9/9) serotypes were recovered from ground turkey. The results indicate that turkey ground meat and meat parts were contaminated with quite distinct Salmonella serotypes. This is the first study reporting Salmonella serotype distribution in turkey meat and S. Corvallis as predominant serotype in poultry meat in Turkey.

Molecular typing of Clostridium perfringens isolated from turkey meat by multiplex PCR

Aims:  To determine the presence of toxin genes in 22 Clostridium perfringens isolated from turkey meat samples by molecular typing.

Isolation and genomic characterization of Escherichia coli O157:H7 in bile of cattle

A total of 200 bile samples of cattle collected from two slaughterhouses from May to September 2007 were analyzed by immunomagnetic separation (IMS)-based cultivation technique. Two (1.0%) of the samples were found to be contaminated with E. coli O157:H7. One of the two isolates was found to carry all five virulence genes (fliCh7, stx1, stx2, eaeA and hly), and the other isolate was positive for these genes except stx2 by multiplex PCR. According to the PFGE, the strains isolated from two different bile samples displayed different XbaI REDP. Antibiotic susceptibility profiles indicated that two isolates were resistant to cephalothin and intermediately resistant to ampicillin, streptomycin and sulphamethoxazole. Also, one isolate was intermediately resistant to kanamycin. It is concluded that the gallbladder should be considered as a possible site and source of E. coli O157:H7 in cattle.

Antibiotic resistance of Escherichia coli O157:H7 isolated from cattle and sheep

A total of 102 Escherichia coli O157:H7 colonies recovered from 11 cattle and 14 sheep were collected and tested for their antibiotic resistance profiles using a disc diffusion method, according to the Clinical and Laboratory Standards Institute. Four (36.36 %) of the 11 cattle E. coli O157:H7 isolates were resistant to cephalothin, one (9.09 %) isolate was resistant to streptomycin, and one (9.09 %) to nalidixic acid. Two (14.28 %) of the 14 sheep E. coli O157:H7 isolates were resistant to sulphamethoxazole, one (7.14 %) isolate was resistant to sulphonamide compounds, and one (7.14 %) to streptomycin. All cattle and sheep isolates were found to be susceptible to cephazolin, gentamicin, ciprofloxacin, imipenem, trimethoprim/ sulphamethoxazole, chloramphenicol, trimethoprim, and ceftiofur. Six cattle isolates were susceptible at a ratio of 54.54 %, and 11 (78.57 %) isolates from sheep were susceptible to all 20 antibiotics tested. As an overall result, 68 % of the E. coli O157:H7 isolates belonging to cattle and sheep were susceptible to all antibiotics tested. On the other hand, most of the E. coli O157:H7 isolates were intermediately resistant to streptomycin, cephalothin, sulphamethoxazole, ampicillin, and kanamycin.

Comparison of hipO and ceuE Gene Based PCR Assays for the Detection of Campylobacter Jejuni

Objective: To evaluate Plavix impact on the perfusion of the uterine artery compared to low dose aspirin in women with unexplained recurrent miscarriage