Muhammad Zia ur Rehman

Contrasting effects of biochar, compost and farm manure on alleviation of nickel toxicity in maize (Zea mays L.) in relation to plant growth, photosynthesis and metal uptake

Nickel (Ni) toxicity in agricultural crops is a widespread problem while little is known about the role of biochar (BC) and other organic amendments like farm manure (FM) from cattle farm and compost (Cmp) on its alleviation. A greenhouse experiment was conducted to evaluate the effects of BC, Cmp and FM on physiological and biochemical characteristics of maize (Zea mays L.) under Ni stress. Maize was grown in Ni spiked soil without and with two rates of the amendments (equivalent to 1% and 2% organic carbon, OC) applied separately to the soil. After harvest, plant height, root length, dry weight, chlorophyll contents, gas exchange characteristics and trace elements in plants were determined. In addition, post-harvest soil characteristics like pHs, ECe and bioavailable Ni were also determined. Compared to the control, all of the amendments increased plant height, root length, shoot and root dry weight with the maximum increase in all parameters by FM (2% OC) treatment. Similarly, total chlorophyll contents and gas exchange characteristics significantly increased with the application of amendments being maximum with FM (2% OC) application. Amendments significantly increased copper, zinc, manganese and iron concentrations and decreased Ni concentrations in the plants. The highest reduction in shoot Ni concentration was recorded with FM (2% OC) followed by BC (2% OC) being 73.2% and 61.1% lower compared to the control, respectively. The maximum increase in soil pH and decrease in AB-DTPA extractable Ni was recorded with BC (2% OC) followed by FM (2% OC). It is concluded that FM (2% OC) was the most effective in reducing Ni toxicity to plants by reducing Ni uptake while BC (2% OC) was the most effective in decreasing bioavailable Ni in the soil through increasing soil pH. However, long-term field studies are needed to evaluate the effects of these amendments in reducing Ni toxicity in plants.

Residual effects of monoammonium phosphate, gypsum and elemental sulfur on cadmium phytoavailability and translocation from soil to wheat in an effluent irrigated field

Cadmium (Cd) accumulation in agricultural soils is one of the major threats to food security. The application of inorganic amendments such as mono-ammonium phosphate (MAP), gypsum and elemental sulfur (S) could alleviate the negative effects of Cd in crops. However, their long-term residual effects on decreasing Cd uptake in latter crops remain unclear. A field that had previously been applied with treatments including control and 0.2, 0.4 and 0.8% by weight of each MAP, gypsum and S, and grown with wheat and rice and thereafter wheat in the rotation was selected for this study. Wheat (Triticum aestivum L.) was grown in the same field as the third crop without further application of amendments to evaluate the residual effects of the amendments on Cd uptake by wheat. Plants were harvested at maturity and grain, and straw yield along with Cd concentration in soil, straw, and grains was determined. The addition of MAP and gypsum significantly increased wheat growth and yield and decreased Cd accumulation in straw and grains compared to control while the reverse was found in S application. Both MAP and gypsum decreased AB-DTPA extractable Cd in soil while S increased the bioavailable Cd in soil. Both MAP and gypsum increased the Cd immobilization in the soil and S decreased Cd immobilization in a dose-additive manner. We conclude that MAP and gypsum had a significant residual effect on decreasing Cd uptake in wheat. The cost-benefit ratio revealed that gypsum is an effective amendment for decreasing Cd concentration in plants.

Genetic Variation in Cadmium Accumulation and Tolerance among Wheat Cultivars at the Seedling Stage

ABSTRACT Exploitation of genetic differences to select wheat cultivars’ pollution-safe from cadmium (Cd) contamination requires better understanding of Cd uptake and translocation patterns. For this purpose, 15 wheat cultivars were grown in nutrient solution and exposed to four levels of Cd, i.e., 0, 15, 30, and 45 µM. The plants were harvested after 2 weeks of Cd exposure. Root and shoot relative dry matter (DM) was decreased in most of the cultivars, but some cultivars did not exhibit any toxic symptoms. The lowest Cd concentration in shoots was recorded for Lasani-2008 and Iqbal-2000 while the highest for Sehar-2006 and Inqlab-91. Both root absorption and translocation accounted for regulating Cd concentration in shoots. There was no relationship between relative DM and Cd concentrations in roots and shoots. The results suggest that wheat cultivars with low shoot Cd concentration but higher tolerance, i.e. Lasani-2008 and Iqbal-2000, could be used in breading programs for low Cd wheat.

Remediation of heavy metal contaminated soils by using Solanum nigrum: A review

Heavy metals are among the major environmental pollutants and the accumulation of these metals in soils is of great concern in agricultural production due to the toxic effects on crop growth and food quality. Phytoremediation is a promising technique which is being considered as an alternative and low-cost technology for the remediation of metal-contaminated soils. Solanum nigrum is widely studied for the remediation of heavy metal-contaminated soils owing to its ability for metal uptake and tolerance. S. nigrum can tolerate excess amount of certain metals through different mechanism including enhancing the activities of antioxidant enzymes and metal deposition in non-active parts of the plant. An overview of heavy metal uptake and tolerance in S. nigrum is given. Both endophytic and soil microorganisms can play a role in enhancing metal tolerance in S. nigrum. Additionally, optimization of soil management practices and exogenous application of amendments can also be used to enhance metal uptake and tolerance in this plant. The main objective of the present review is to highlight and discuss the recent progresses in using S. nigrum for remediation of metal contaminated soils.

Cadmium phytoremediation potential of Brassica crop species: A review

Cadmium (Cd) is a highly toxic metal released into the environment through anthropogenic activities. Phytoremediation is a green technology used for the stabilization or remediation of Cd-contaminated soils. Brassica crop species can produce high biomass under a range of climatic and growing conditions, allowing for considerable uptake and accumulation of Cd, depending on species. These crop species can tolerate Cd stress via different mechanisms, including the stimulation of the antioxidant defense system, chelation, compartmentation of Cd into metabolically inactive parts, and accumulation of total amino-acids and osmoprotectants. A higher Cd-stress level, however, overcomes the defense system and may cause oxidative stress in Brassica species due to overproduction of reactive oxygen species and lipid peroxidation. Therefore, numerous approaches have been followed to decrease Cd toxicity in Brassica species, including selection of Cd-tolerant cultivars, the use of inorganic and organic amendments, exogenous application of soil organisms, and employment of plant-growth regulators. Furthermore, the coupling of genetic engineering with cropping may also help to alleviate Cd toxicity in Brassica species. However, several field studies demonstrated contrasting results. This review suggests that the combination of Cd-tolerant Brassica cultivars and the application of soil amendments, along with proper agricultural practices, may be the most efficient means of the soil Cd phytoattenuation. Breeding and selection of Cd-tolerant species, as well as species with higher biomass production, might be needed in the future when aiming to use Brassica species for phytoremediation.

A critical review of mechanisms involved in the adsorption of organic and inorganic contaminants through biochar

Successful application of low-cost adsorbent like biochar has introduced multidisciplinary areas of science, particularly with respect to remediation of both organic and inorganic contaminants in soil and water. This review briefly consolidates the currently available literature to comprehend the overall adsorption mechanisms of organic and inorganic contaminants through biochar. Generally, biochar produced at low pyrolysis temperature is suitable for the adsorption of inorganic contaminants by following the adsorption mechanisms of electrostatic interaction, precipitation, and ion exchange. However, relatively high pyrolysis temperature favors the adsorption of organic contaminants by increasing the surface area and micropores of biochar along with pore filling and hydrophobic and electrostatic interaction as predominant adsorption mechanisms. This review also summarizes the critical factors (pyrolysis temperature, solution pH, dosage of biochar, contact time, co-existed ions, modification/activation, aging process, etc) affecting the overall adsorption efficiency of the biochar. Overall, further field studies and experimentation are required for the reduction of cost in biochar production and its reuse.

Alleviation of cadmium (Cd) toxicity and minimizing its uptake in wheat ( Triticum aestivum ) by using organic carbon sources in Cd-spiked soil

Cadmium (Cd)-contamination of agricultural soils has been receiving attention worldwide due to its entry into food crops such as wheat (Triticum aestivum L.). Little is known regarding the use of organic carbon (OC) sources in alleviating Cd toxicity in cereals. The current experiment was aimed to study the effects of different OC sources on the Cd accumulation by wheat. A pot study was conducted to determine the effects of rice husk biochar (RHB), farmyard manure (FYM), and lignite (LT) either alone or in combination on crop growth, Cd bioavailability and health risk assessment. The results proved that the application of OC sources like RHB, FYM, and LT either alone or in combination were highly effective in enhancing the wheat growth and yield as well as in minimizing the phyto-available fraction of Cd and its transfer to edible tissue of wheat. The RHB was the most efficient source in enhancing the plant growth and reducing the Cd concentration in wheat tissues. RHB increased grain yield by 91% and decreased Cd concentration in shoot, roots, grains, and bioavailable fraction of Cd by 67, 69, 62.5, and 74% than control, respectively. The RHB reduced the daily Cd uptake and health risk index in adults in comparison to control. Overall, where un-amended soil resulted in diminished plant productivity, the application of other OC sources also significantly proved their potential to enhance the dry weight and grain yield, suggesting that these OC sources may be used aiming to minimize the Cd concentration in crops. However, there is still a need to explore the potential of different OC sources in combination with other frequently available amendments for their large scale implementation in metal-contaminated soils.

Zinc and iron oxide nanoparticles improved the plant growth and reduced the oxidative stress and cadmium concentration in wheat

The effects of seed priming with zinc oxide (ZnO) and iron (Fe) nanoparticles (NPs) on the growth and cadmium (Cd) accumulation by wheat (Triticum aestivum) were investigated. Seeds of wheat were primed with different concentrations of either ZnO NPs (0, 25, 50, 75, and 100 mg L-1) or Fe NPs (0, 5, 10, 15, and 20 mg L-1) for 24 h by continuous aeration and then the seeds were sown in a soil which was contaminated with Cd due to long-term application of sewage water. Plants were grown till maturity under natural conditions with 60-70% moisture contents of total soil water holding capacity throughout the experiment. Plant height, spike length, and dry weights of shoots, roots, spikes, and grains were increased with NPs, in particular with the higher rates of NPs. The results depicted that NPs positively affected the photosynthesis of wheat as compared to the control. The NPs reduced the electrolyte leakage and superoxide dismutase and peroxidase activities in leaves of Cd-stressed wheat. The concentrations of Cd in roots, shoots, and grains were significantly decreased with NPs application. The Cd content in the grains was below the threshold level of Cd (0.2 mg kg-1) for cereals when the seeds were treated with higher NPs treatments. The application of ZnO NPs increased the Zn concentrations and Fe NPs increased the Fe concentrations in roots, shoots, and grains. Overall, the NPs play a major role in the increase in biomass, nutrients and decrease in Cd toxicity in wheat.

Lead Toxicity in Cereals and Its Management Strategies: a Critical Review

Cereal grains such as wheat, rice, and maize are widely consumed as a staple food worldwide. Lead (Pb) is one of the non-essential trace elements and its toxicity in crops especially cereals is a widespread problem. The present review highlighted Pb toxicity in cereal and management strategies to reduce its uptake in plants. Lead toxicity reduced the cereal growth, photosynthesis, nutritional value, yield, and grain quality. The response of cereals to excess varies with plant species, levels of Pb in soil, and growth conditions. Reducing Pb bioavailability in the soil is a viable approach due to its non-degradability either by microbes, chemicals, or other means. Cultivation of low Pb-accumulating cultivars may reduce the risk of Pb toxicity in plants and humans via the food chain. Use of plant growth regulators, microbes, organic, and inorganic amendments might be promising techniques for further decreasing Pb contents in shoot and grains. Soil amendments along with selecting low Pb-accumulating cultivars might be a feasible approach to get cereal grains with low Pb concentrations. Furthermore, most of the studies have been conducted under controlled conditions either in hydroponic or pots and less is known about the effects of Pb management approaches under ambient field conditions.

Zinc oxide nanoparticles alter the wheat physiological response and reduce the cadmium uptake by plants

An experiment was performed to explore the interactive impacts of zinc oxide nanoparticles (ZnO NPs) and cadmium (Cd) on growth, yield, antioxidant enzymes, Cd and zinc (Zn) concentrations in wheat (Triticum aestivum). The ZnO NPs were applied both in Cd-contaminated soil and foliar spray (in separate studies) on wheat at different intervals and plants were harvested after physiological maturity. Results depicted that ZnO NPs enhanced the growth, photosynthesis, and grain yield, whereas Cd and Zn concentrations decreased and increased respectively in wheat shoots, roots and grains. The Cd concentrations in the grains were decreased by 30-77%, and 16-78% with foliar and soil application of NPs as compared to the control, respectively. The ZnO NPs reduced the electrolyte leakage while increased SOD and POD activities in leaves of wheat. It can be concluded that ZnO NPs (levels used in the study) could effectively reduce the toxicity and concentration of Cd in wheat whereas increase the Zn concentration in wheat. Thus, ZnO NPs might be helpful in decreasing Cd and increasing Zn biofortification in cereals which might be effective to reduce the hidden hunger in humans owing the deficiency of Zn in cereals.