Munechika Sakabe

Development of Automatic Technics of Purification Process of M13 Single Strand DNA and Sequence Reaction Based on Sanger Method

Publisher Summary This chapter presents the method of automatization of purification process of M13 single-strand DNA and the automatization of dideoxy sequence reaction based on the Sanger method. The chapter discusses the process of developing the device that automatically purifies DNA fragments. The chapter also describes the process of developing the device that automatically processes the dideoxy sequence reaction. Both of these devices are driven by the computer; therefore, all of their functions are programmable. The autoradiogram pattern derived from automatical purification corresponds with the pattern derived from manual purification. However, when the protein or phenol is not removed completely in the purification process, the pattern shows background. That is the main problem in automatization of DNA purification. Therefore, phenol extraction should be improved at first.